BBa_K592100
1
BFP
Blue Fluorescent Protein (mTagBFP)
2011-09-19T11:00:00Z
2016-01-25T04:45:27Z
Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Released HQ 2013
This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
false
_763_
4206
10137
9
In stock
true
The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
Erik Gullberg
annotation2135490
1
mTagBFP
range2135490
1
1
699
BBa_K1415009
1
BBa_K1415009
PBAN (Aedes aegypti)
2014-10-02T11:00:00Z
2015-05-08T01:10:19Z
Artificial synthesis
Yellow fever mosquito (Aedes aegypyi)
Spread:The yellow fever mosquito, Aedes aegypti, is a mosquito that can spread the dengue fever, chikungunya, and yellow fever viruses, and other diseases. The mosquito can be recognized by white markings on its legs and a marking in the form of a lyre on the thorax. The mosquito originated in Africa, but is now found in tropical and subtropical regions throughout the world.
Characteristics: The mosquito can be recognized by white markings on its legs and a marking in the form of a lyre on the thorax.
Damage: The yellow fever mosquito, Aedes aegypti, is a mosquito that can spread the dengue fever, chikungunya, and yellow fever viruses, and other diseases.
Control:(1) Empty water from containers such as flower pots, birdbaths, pet water dishes, cans, gutters, tires and buckets regularly to disrupt the mosquito breeding cycle. (2)Consider using an insect repellent, be sure to follow the label directions for applying the repellent. For help selecting a mosquito repellent, try our Insect Repellent Locator.
false
false
_1793_
0
22512
9
In stock
false
No
false
HO, TSUNG YU
annotation2392811
1
PBAN (Aedes aegypti)
range2392811
1
1
64
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1415209
1
BBa_K1415209
Pcons+B0034+PBAN(Aedes aegypti)+B0034+BFP+J61048
2014-10-04T11:00:00Z
2015-05-08T01:10:20Z
Assemble
We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.
false
false
_1793_
0
22512
9
It's complicated
false
No
false
HO, TSUNG YU
component2394101
1
BBa_K1415009
component2394106
1
BBa_J61048
component2394103
1
BBa_B0034
component2394099
1
BBa_B0034
component2394105
1
BBa_K592100
component2394097
1
BBa_J23101
annotation2394103
1
BBa_B0034
range2394103
1
134
145
annotation2394101
1
BBa_K1415009
range2394101
1
62
125
annotation2394099
1
BBa_B0034
range2394099
1
44
55
annotation2394105
1
BBa_K592100
range2394105
1
152
856
annotation2394097
1
BBa_J23101
range2394097
1
1
35
annotation2394106
1
BBa_J61048
range2394106
1
865
977
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_K1415209_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatggacgcgagcagtagcaacgaaaataacagccgcccgccgtttgccccgcgtctgtaataattactagagaaagaggagaaatactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_K1415009_sequence
1
atggacgcgagcagtagcaacgaaaataacagccgcccgccgtttgccccgcgtctgtaataat
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K592100_sequence
1
atgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z