LOCUS       BBa_K1416000            1738 bp    DNA     linear       04-May-2024
DEFINITION  The tRNA synthetase/tRNA needed for incorporating 
            o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon
ACCESSION   BBa_K1416000
VERSION     BBa_K1416000.1  
FEATURES             Location/Qualifiers
     promoter        72..219
                     /label=ONBYRS promoter
     CDS             233..1153
                     /label=ONBYRS
     promoter        1330..1379
                     /label=tRNA promoter
     CDS             1396..1471
                     /label=tRNA
     stem_loop       1497..1526
                     /label=terminator
ORIGIN
        1 gcacctgaag tcagccccat acgatataag ttgtaattct catgtttgac agcattatca
       61 tcgataagct tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
      121 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
      181 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac ttggagattc atatggacga
      241 gttcgagatg atcaaacgca acaccagcga aatcatcagc gaagaagaac tgcgcgaagt
      301 gctgaaaaaa gacgagaaga gcgccggcat tggcttcgaa ccgagcggca aaattcatct
      361 gggccactac ctgcaaatca agaagatgat cgatctgcaa aacgcgggct tcgatatcat
      421 cattggcctg gccgatctgc atgcatacct gaaccagaaa ggcgaactgg acgaaatccg
      481 caaaatcggc gactacaaca agaaagtgtt cgaagcgatg ggcctgaaag cgaaatacgt
      541 ctatggcagc gaagaacagc tggacaaaga ctacaccctg aacgtgtatc gtctggcact
      601 gaaaaccacc ctgaaacgtg ctcgtcgttc tatggaactg attgcccgcg aagatgaaaa
      661 cccgaaagtg gcggaagtga tctatccgat catgcaggtg aacagcatcc actatgaagg
      721 cgtagatgta gcagttggtg gcatggaaca gcgcaaaatc cacatgctgg ctcgtgaact
      781 gctgccgaaa aaagtcgtgt gcatccacaa cccggtttta acgggtctgg atggtgaagg
      841 caaaatgagc agcagcaaag gcaactttat cgccgtggat gattctccgg aagaaatccg
      901 cgcgaagatc aaaaaagcgt attgcccggc aggtgtagtt gaaggtaacc cgatcatgga
      961 gatcgcgaaa tacttcctgg aatacccgct gaccattaaa cgcccggaaa aattcggtgg
     1021 tgatctgacc gtgaacagct acgaagaact ggagagcctg ttcaagaaca aagaactgca
     1081 cccgatgcgt ctgaaaaatg ccgttgcgga agagctgatc aaaatcctgg aaccgattcg
     1141 caaacgtctg taagatctgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     1201 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     1261 gccacttcgg gctcatgagg catgcggcgc cgcttctttg agcgaacgat caaaaataag
     1321 tggcgcccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
     1381 gccatcagac gcattccggc ggtagttcag cagggcagaa cggcggactc taaatccgca
     1441 ggtcgctggt tcaaatccgg cccgccggac catttatcac agattggaaa tttttgatcc
     1501 ttagcgaaag ctaaggattt tttttagtcg accgatgccc ttgagagcct tcaacccagt
     1561 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1621 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1681 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcac
//