BBa_K1416000 1 ONBY RS The tRNA synthetase/tRNA needed for incorporating o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon 2014-10-05T11:00:00Z 2015-05-08T01:10:20Z Evolved from the Methanocaldococcus janaschii tyrosyl synthetase and tRNA by the Schulz group. Specific mutations sourced from Deiters, A., Groff, D., Ryu, Y., Xie, J. and Schultz, P. G. (2006), A Genetically Encoded Photocaged Tyrosine. Angew. Chem. Int. Ed., 45: 2728???2731. The part includes the gene coding for the Methanocaldococcus jannaschii synthetase mutated to charge the orthogonal tRNA (also in the part) with the non-canonical amino acid <i>o</i>-nitrobenzyl tyrosine (ONBY) along with the proper promoters and terminator . This part encodes for the incorporation of ONBY during translation at AUG amber stop codons in a cell with Release Factor 1 (RF-1) knocked out such as "Amberless" <i>E. coli</i> or "RF-0". false false _1794_ 0 17383 9 In stock true false Jordan Monk annotation2426651 1 ONBYRS promoter range2426651 1 72 219 annotation2426655 1 terminator range2426655 1 1497 1526 annotation2426654 1 tRNA range2426654 1 1396 1471 annotation2426652 1 ONBYRS range2426652 1 233 1153 annotation2426653 1 tRNA promoter range2426653 1 1330 1379 BBa_K1416000_sequence 1 gcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcattatcatcgataagcttgatgcgtggaagattgatcgtcttgcaccctgaaaagatgcaaaaatcttgctttaatcgctggtactcctgattctggcactttattctatgtctctttcgcatctggcgaaaagtcgtgtaccggcaaaggtgcagtcgttatatacttggagattcatatggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgccggcattggcttcgaaccgagcggcaaaattcatctgggccactacctgcaaatcaagaagatgatcgatctgcaaaacgcgggcttcgatatcatcattggcctggccgatctgcatgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaagaacagctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacagcatccactatgaaggcgtagatgtagcagttggtggcatggaacagcgcaaaatccacatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgcgtctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaagatctggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgaggcatgcggcgccgcttctttgagcgaacgatcaaaaataagtggcgccccatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgctgccatcagacgcattccggcggtagttcagcagggcagaacggcggactctaaatccgcaggtcgctggttcaaatccggcccgccggaccatttatcacagattggaaatttttgatccttagcgaaagctaaggattttttttagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z