BBa_K1416001 1 IodoY RS The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon 2014-10-05T11:00:00Z 2015-05-08T01:10:20Z This part is from the genomic sequence of <i>Methanocaldoccus jannaschii</i>. Wang L, et al. Expanding the Genetic Code of Escherichia coli. Science 20 April 2001: 292 (5516), 498-500. The part includes the gene coding for the <i>Methanocaldococcus jannaschii</i> synthetase that charges the orthogonal tRNA (also in the part) with the amino acid tyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0". false false _1794_ 0 17383 9 It's complicated false false Jordan Monk annotation2399522 1 IodoYRS range2399522 1 233 1153 annotation2426634 1 tRNA promoter range2426634 1 1330 1379 annotation2399464 1 IodoYRS promoter range2399464 1 72 219 annotation2426635 1 tRNA range2426635 1 1396 1471 annotation2426650 1 terminator range2426650 1 1497 1526 BBa_K1416001_sequence 1 gcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcattatcatcgataagcttgatgcgtggaagattgatcgtcttgcaccctgaaaagatgcaaaaatcttgctttaatcgctggtactcctgattctggcactttattctatgtctctttcgcatctggcgaaaagtcgtgtaccggcaaaggtgcagtcgttatatacttggagattcatatggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgcctatattggcttcgaaccgagcggcaaaattcatctgggccactacctgcaaatcaagaagatgatcgatctgcaaaacgcgggcttcgatatcatcattctgctggccgatctggccgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaatttcagctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacaccagccactatctgggcgtagatgtagcagttggtggcatggaacagcgcaaaatccacatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgtatctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaagatctggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgaggcatgcggcgccgcttctttgagcgaacgatcaaaaataagtggcgccccatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgctgccatcagacgcattccggcggtagttcagcagggcagaacggcggactctaaatccgcaggtcgctggttcaaatccggcccgccggaccatttatcacagattggaaatttttgatccttagcgaaagctaaggattttttttagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z