BBa_K1416001
1
IodoY RS
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
2014-10-05T11:00:00Z
2015-05-08T01:10:20Z
This part is from the genomic sequence of <i>Methanocaldoccus jannaschii</i>. Wang L, et al. Expanding the Genetic Code of Escherichia coli. Science 20 April 2001: 292 (5516), 498-500.
The part includes the gene coding for the <i>Methanocaldococcus jannaschii</i> synthetase that charges the orthogonal tRNA (also in the part) with the amino acid tyrosine along with the proper promoters and terminator. This part encodes for the incorporation of tyrosine during translation at AUG amber stop codons in a cell with Release Factor 1 (RF1) knocked out such as "Amberless" E. coli or "RF0".
false
false
_1794_
0
17383
9
It's complicated
false
false
Jordan Monk
annotation2399522
1
IodoYRS
range2399522
1
233
1153
annotation2426634
1
tRNA promoter
range2426634
1
1330
1379
annotation2399464
1
IodoYRS promoter
range2399464
1
72
219
annotation2426635
1
tRNA
range2426635
1
1396
1471
annotation2426650
1
terminator
range2426650
1
1497
1526
BBa_K1416001_sequence
1
gcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcattatcatcgataagcttgatgcgtggaagattgatcgtcttgcaccctgaaaagatgcaaaaatcttgctttaatcgctggtactcctgattctggcactttattctatgtctctttcgcatctggcgaaaagtcgtgtaccggcaaaggtgcagtcgttatatacttggagattcatatggacgagttcgagatgatcaaacgcaacaccagcgaaatcatcagcgaagaagaactgcgcgaagtgctgaaaaaagacgagaagagcgcctatattggcttcgaaccgagcggcaaaattcatctgggccactacctgcaaatcaagaagatgatcgatctgcaaaacgcgggcttcgatatcatcattctgctggccgatctggccgcatacctgaaccagaaaggcgaactggacgaaatccgcaaaatcggcgactacaacaagaaagtgttcgaagcgatgggcctgaaagcgaaatacgtctatggcagcgaatttcagctggacaaagactacaccctgaacgtgtatcgtctggcactgaaaaccaccctgaaacgtgctcgtcgttctatggaactgattgcccgcgaagatgaaaacccgaaagtggcggaagtgatctatccgatcatgcaggtgaacaccagccactatctgggcgtagatgtagcagttggtggcatggaacagcgcaaaatccacatgctggctcgtgaactgctgccgaaaaaagtcgtgtgcatccacaacccggttttaacgggtctggatggtgaaggcaaaatgagcagcagcaaaggcaactttatcgccgtggatgattctccggaagaaatccgcgcgaagatcaaaaaagcgtattgcccggcaggtgtagttgaaggtaacccgatcatggagatcgcgaaatacttcctggaatacccgctgaccattaaacgcccggaaaaattcggtggtgatctgaccgtgaacagctacgaagaactggagagcctgttcaagaacaaagaactgcacccgatgtatctgaaaaatgccgttgcggaagagctgatcaaaatcctggaaccgattcgcaaacgtctgtaagatctggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgaggcatgcggcgccgcttctttgagcgaacgatcaaaaataagtggcgccccatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgctgccatcagacgcattccggcggtagttcagcagggcagaacggcggactctaaatccgcaggtcgctggttcaaatccggcccgccggaccatttatcacagattggaaatttttgatccttagcgaaagctaaggattttttttagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z