LOCUS BBa_K1416004 5966 bp DNA linear 26-Nov-2024 DEFINITION pFRY: a Reporter Plasmid for Measuring ncAA Incorporation ACCESSION BBa_K1416004 VERSION BBa_K1416004.1 FEATURES Location/Qualifiers ORIGIN 1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc 421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 661 cactccaccg gtgctatggg atccgctggc tccgctgctg gttctggcga ataggcatcc 721 aagggcgagg agctctttac tggcgtagta ccaattctcg tagagctcga tggcgatgta 781 aatggccata agttttccgt acgcggcgag ggcgagggcg atgcaactaa cggcaagctc 841 actctcaagt ttatttgtac tactggcaag ctcccagtac catggccaac tctcgtaact 901 actctgacct atggcgtaca atgtttttcc cgctatccag atcacatgaa gcaacatgat 961 ttttttaagt ccgcaatgcc agagggctat gtacaagagc gcactattag ctttaaggat 1021 gatggcacct ataagactcg cgcagaggta aagtttgagg gcgatactct cgtaaatcgc 1081 attgagctca agggcattga ttttaaggag gatggcaata ttctcggcca taagctggag 1141 tataatttca attcccataa tgtatatatt accgcagata agcaaaagaa tggcattaag 1201 gcgaatttta agattcgcca taatgtggag gatggctccg tacaactcgc agatcattat 1261 caacaaaata ctccaattgg cgatggccca gtactcctcc cagataatca ttatctctcc 1321 actcaatccg tgctctccaa agatccaaat gagaagcgcg atcacatggt actcctggag 1381 tttgtaactg cagcaggcat tactcatggc atggatgagc tctataagct cgagcaccac 1441 caccaccacc actaaggatc cgcatgcgag ctcggtaccc cgggtcgacc tgcagccaag 1501 cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc 1561 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct 1621 agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 1681 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 1741 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 1801 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 1861 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 1921 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 1981 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 2041 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 2101 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 2161 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 2221 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 2281 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc 2341 ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac gaaaggctca 2401 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag 2461 gacaaatccg ccctctagat tacgtgcagt cgatgataag ctgtcaaaca tgagaattgt 2521 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg 2581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 2641 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc 2701 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag 2761 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc 2821 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg 2881 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc 2941 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc 3001 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg 3061 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac 3121 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg 3181 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc 3241 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag 3301 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac 3361 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc 3421 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg 3481 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg 3541 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac 3601 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg 3661 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc 3721 ggaatttcgg gcagcgttgg gtcctggcca cgggtgcgca tgatctagag ctgcctcgcg 3781 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 3841 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 3901 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta 3961 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 4021 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 4081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 4441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat ggcgttaagg gattttggtc 4921 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc 4981 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc 5041 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac 5101 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc 5161 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg 5221 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt 5281 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca 5341 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc 5401 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag 5461 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc 5521 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc 5581 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc 5641 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga 5701 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt 5761 attactgttt atgtaagcag acagttttat tgttcatgac catgacatta acctataaaa 5821 ataggcgtat cacgaggccc tttcgtcttc acctcgagaa atcataaaaa atttatttgc 5881 tttgtgagcg gataacaatt ataatagatt caattgtgag cggataacaa tttcacacag 5941 aattcattaa agaggagaaa ttaact //