BBa_K734002 1 BBa_K734002 Spinach Aptamer with Stabilizing tRNA Scaffold 2012-08-21T11:00:00Z 2015-05-08T01:13:07Z Generated from SELEX. The fluorescent Spinach Aptamer (SpA) created by the Jaffrey Lab at Cornell University, designed to bind to the GFP-like synthetic fluorophore, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). SpA generates a green fluorescence upon binding DFHBI after transcription into mRNA. As reported by the Jaffrey group; excitation wavelength: 469 nm, emission wavelength: 501, with 53% EGFP fluorescent strength. false false _983_ 0 13725 9 Not in stock false false Logan Bachman annotation2180552 1 tRNA Scaffold (left) range2180552 1 1 30 annotation2180553 1 tRNA Scaffold (right) range2180553 1 111 145 annotation2180554 1 Spinach RNA Aptamer range2180554 1 31 110 BBa_K1417000 1 BBa_K1417000 Red Fluorescent Protein - Spacer - Spinach Aptamer 2014-10-08T11:00:00Z 2015-05-08T01:10:21Z This part was created synthetically. Produces green fluorescent RNA and red fluorescent protein for quantitative measurement of promoter and RBS activity. false false _1795_ 0 22701 9 Not in stock false Incorporation of RBS and Promoter was a design challenge. false Adam Baker component2407914 1 BBa_K734002 component2407910 1 BBa_K1362461 annotation2407914 1 BBa_K734002 range2407914 1 715 859 annotation2407910 1 BBa_K1362461 range2407910 1 1 706 BBa_K1362461 1 BBa_K1362461 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode 2014-10-07T11:00:00Z 2016-01-25T01:30:37Z ''Discosoma striata'' This part is essentially identical to <partinfo>BBa_E1010</partinfo>, but has a point mutation in the barcode. Barcodes are discontinued, but composite parts using this part will have the mutated barcode. false false _1738_ 4206 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2404599 1 mrfp1 range2404599 1 1 675 annotation2404604 1 mutated barcode range2404604 1 682 706 BBa_K1417000_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgatgatagtgctagtgtagatcgctactagaggaagcggtggctcaatggtagagctttcgagacgcgaccgaaatggtgaaggacgggtccagtgcttcggcactgttgagtagagtgtgagctccgtaactggtcgcgtctcgaagggttgcaggttcaattcctgtccgtttca BBa_K734002_sequence 1 gaagcggtggctcaatggtagagctttcgagacgcgaccgaaatggtgaaggacgggtccagtgcttcggcactgttgagtagagtgtgagctccgtaactggtcgcgtctcgaagggttgcaggttcaattcctgtccgtttca BBa_K1362461_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgatgatagtgctagtgtagatcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z