BBa_K1417003 1 BBa_K1417003 Red Fluorescent Protein - Spacer - Spinach Aptamer 2014-10-08T11:00:00Z 2015-05-08T01:10:21Z Synthetic Produces green fluorescent RNA and red fluorescent protein for quantitative measurement of promoter and RBS activity. false false _1795_ 0 22701 9 It's complicated false Adding in appropriate RBS and promoter sites was a design challenge false Adam Baker component2407961 1 BBa_K1362461 component2407962 1 BBa_K1417001 component2407966 1 BBa_K734002 annotation2407962 1 BBa_K1417001 range2407962 1 715 747 annotation2407966 1 BBa_K734002 range2407966 1 756 900 annotation2407961 1 BBa_K1362461 range2407961 1 1 706 BBa_K1417001 1 BBa_K1417001 Spacer 2014-10-08T11:00:00Z 2015-05-08T01:10:21Z Synthetic This is a spacer used to separate elements in combinatory RNA false false _1795_ 0 22701 9 Not in stock false The spacer has to be of sufficient length that the two RNA pieces do not interfere with each other and does not have any secondary structure. false Adam Baker BBa_K1362461 1 BBa_K1362461 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode 2014-10-07T11:00:00Z 2016-01-25T01:30:37Z ''Discosoma striata'' This part is essentially identical to <partinfo>BBa_E1010</partinfo>, but has a point mutation in the barcode. Barcodes are discontinued, but composite parts using this part will have the mutated barcode. false false _1738_ 4206 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2404599 1 mrfp1 range2404599 1 1 675 annotation2404604 1 mutated barcode range2404604 1 682 706 BBa_K734002 1 BBa_K734002 Spinach Aptamer with Stabilizing tRNA Scaffold 2012-08-21T11:00:00Z 2015-05-08T01:13:07Z Generated from SELEX. The fluorescent Spinach Aptamer (SpA) created by the Jaffrey Lab at Cornell University, designed to bind to the GFP-like synthetic fluorophore, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). SpA generates a green fluorescence upon binding DFHBI after transcription into mRNA. As reported by the Jaffrey group; excitation wavelength: 469 nm, emission wavelength: 501, with 53% EGFP fluorescent strength. false false _983_ 0 13725 9 Not in stock false false Logan Bachman annotation2180552 1 tRNA Scaffold (left) range2180552 1 1 30 annotation2180553 1 tRNA Scaffold (right) range2180553 1 111 145 annotation2180554 1 Spinach RNA Aptamer range2180554 1 31 110 BBa_K1417003_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgatgatagtgctagtgtagatcgctactagaggcccggatagctcagtcggtagagcagcggccgtactagaggaagcggtggctcaatggtagagctttcgagacgcgaccgaaatggtgaaggacgggtccagtgcttcggcactgttgagtagagtgtgagctccgtaactggtcgcgtctcgaagggttgcaggttcaattcctgtccgtttca BBa_K734002_sequence 1 gaagcggtggctcaatggtagagctttcgagacgcgaccgaaatggtgaaggacgggtccagtgcttcggcactgttgagtagagtgtgagctccgtaactggtcgcgtctcgaagggttgcaggttcaattcctgtccgtttca BBa_K1417001_sequence 1 gcccggatagctcagtcggtagagcagcggccg BBa_K1362461_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgatgatagtgctagtgtagatcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z