LOCUS       BBa_K1418002            1364 bp    DNA     linear       22-Nov-2024
DEFINITION  Chlorophyllase (TaCHL) generator with C-terminal 10x His tag
ACCESSION   BBa_K1418002
VERSION     BBa_K1418002.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=atg
     CDS             230..1183
                     /label=chlorophyllase
     CDS             227..1183
                     /label=BBa_K1418001
     misc_feature    1192..1221
                     /label=10x-Histidine Tag
     misc_feature    1222..1224
                     /label=Stop
     misc_feature    1225..1227
                     /label=Stop
     misc_feature    1192..1227
                     /label=BBa_K844000
     stem_loop       1247..1290
                     /label=stem_loop
     misc_feature    1236..1315
                     /label=BBa_B0010
     terminator      1236..1315
                     /label=BBa_B0010
     stem_loop       1331..1350
                     /label=T7 TE
     misc_feature    1357..1357
                     /label=stop
     polyA_site      1351..1364
                     /label=polya
     misc_feature    1324..1364
                     /label=BBa_B0012
     terminator      1324..1364
                     /label=BBa_B0012
     terminator      1236..1364
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cagcagcagc
      241 acccgcagag acgatgaaca agtccgccgc cggcgccgag gttcccgagg cgttcacatc
      301 ggtgttccag ccggggaagc ttgcggtcga ggcgattcag gtggatgaga atgcggcgcc
      361 gacaccaccg atcccggtgc tgatcgtcgc acccaaggat gcaggaacct accccgtggc
      421 catgctcttg cacggcttct tcctccataa ccacttctac gaacaccttc tccggcacgt
      481 cgcatcccac ggcttcatca ttgtcgcgcc ccagttcagc atcagtatca taccttcggg
      541 tgacgcagag gacatcgccg cggcagccaa ggtggcagac tggctccccg acggcctccc
      601 gtccgtgctg cccaaaggcg tcgagccgga gctctcgaag ctcgccttgg ccggccacag
      661 ccgaggaggc cacacggctt tctccctggc cttggggcac gccaagaccc agctaacctt
      721 ctccgcgctc atcggactcg accccgtcgc cggcacgggg aagtcctccc agctccagcc
      781 caagatcctc acctacgagc cgtcctcctt cggcatggcg atgccggtgc tggtcatcgg
      841 caccgggctc ggcgaggaga agaagaacat attcttccct ccctgcgcac ccaaggacgt
      901 gaaccacgcc gagttctacc gcgagtgcag gccgccctgc tactactttg tgaccaagga
      961 ctacgggcat ctggacatgc tggacgacga cgcccccaag ttcatcacct gcgtctgcaa
     1021 ggatgggaac gggtgcaagg gcaagatgcg gaggtgcgtt gctgggatca tggtggcatt
     1081 tcttaatgct gccttgggtg agaaagatgc agatcttgag gccatactga gagacccggc
     1141 ggttgcaccc accacgcttg atccggttga gcaccgcgtg gcgtactaga gcatcatcac
     1201 catcaccacc atcatcacca ttaataatac tagagccagg catcaaataa aacgaaaggc
     1261 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1321 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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