BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206609 1 Stop range2206609 1 34 36 annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206608 1 Stop range2206608 1 31 33 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K208010 1 BBa_K208010 Lac Promoter (BBa_R0010) and RBS (B0034) 2009-10-11T11:00:00Z 2015-05-08T01:11:24Z synthetic Released HQ 2013 lac + rbs false false _310_ 0 3473 9 In stock true none false Elisabeth Linton annotation2034625 1 BBa_B0034 range2034625 1 209 220 annotation2034626 1 BBa_R0010 range2034626 1 1 200 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_K1418001 1 BBa_K1418001 Chlorophyllase (TaCHL from <i>Triticum aestivum</i>) with stop codons removed 2014-10-07T11:00:00Z 2015-05-08T01:10:21Z TaCHL Chlorophyllase(ns) false false _1796_ 0 13965 9 It's complicated false RFC 23 false Ryan Putman annotation2421172 1 chlorophyllase range2421172 1 4 957 annotation2421171 1 atg range2421171 1 1 3 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1418002 1 BBa_K1418002 Chlorophyllase (TaCHL) generator with C-terminal 10x His tag 2014-10-07T11:00:00Z 2015-05-08T01:10:21Z TaCHL Chlorophyllase producing construct with C-terminal 10x His-Tag false false _1796_ 0 13965 9 It's complicated true RFC 23 false Ryan Putman component2404154 1 BBa_K1418001 component2404153 1 BBa_K208010 component2404158 1 BBa_K844000 component2404165 1 BBa_B0015 annotation2404158 1 BBa_K844000 range2404158 1 1192 1227 annotation2404165 1 BBa_B0015 range2404165 1 1236 1364 annotation2404154 1 BBa_K1418001 range2404154 1 227 1183 annotation2404153 1 BBa_K208010 range2404153 1 1 220 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1418002_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcagcagcagcacccgcagagacgatgaacaagtccgccgccggcgccgaggttcccgaggcgttcacatcggtgttccagccggggaagcttgcggtcgaggcgattcaggtggatgagaatgcggcgccgacaccaccgatcccggtgctgatcgtcgcacccaaggatgcaggaacctaccccgtggccatgctcttgcacggcttcttcctccataaccacttctacgaacaccttctccggcacgtcgcatcccacggcttcatcattgtcgcgccccagttcagcatcagtatcataccttcgggtgacgcagaggacatcgccgcggcagccaaggtggcagactggctccccgacggcctcccgtccgtgctgcccaaaggcgtcgagccggagctctcgaagctcgccttggccggccacagccgaggaggccacacggctttctccctggccttggggcacgccaagacccagctaaccttctccgcgctcatcggactcgaccccgtcgccggcacggggaagtcctcccagctccagcccaagatcctcacctacgagccgtcctccttcggcatggcgatgccggtgctggtcatcggcaccgggctcggcgaggagaagaagaacatattcttccctccctgcgcacccaaggacgtgaaccacgccgagttctaccgcgagtgcaggccgccctgctactactttgtgaccaaggactacgggcatctggacatgctggacgacgacgcccccaagttcatcacctgcgtctgcaaggatgggaacgggtgcaagggcaagatgcggaggtgcgttgctgggatcatggtggcatttcttaatgctgccttgggtgagaaagatgcagatcttgaggccatactgagagacccggcggttgcacccaccacgcttgatccggttgagcaccgcgtggcgtactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1418001_sequence 1 atggcagcagcagcacccgcagagacgatgaacaagtccgccgccggcgccgaggttcccgaggcgttcacatcggtgttccagccggggaagcttgcggtcgaggcgattcaggtggatgagaatgcggcgccgacaccaccgatcccggtgctgatcgtcgcacccaaggatgcaggaacctaccccgtggccatgctcttgcacggcttcttcctccataaccacttctacgaacaccttctccggcacgtcgcatcccacggcttcatcattgtcgcgccccagttcagcatcagtatcataccttcgggtgacgcagaggacatcgccgcggcagccaaggtggcagactggctccccgacggcctcccgtccgtgctgcccaaaggcgtcgagccggagctctcgaagctcgccttggccggccacagccgaggaggccacacggctttctccctggccttggggcacgccaagacccagctaaccttctccgcgctcatcggactcgaccccgtcgccggcacggggaagtcctcccagctccagcccaagatcctcacctacgagccgtcctccttcggcatggcgatgccggtgctggtcatcggcaccgggctcggcgaggagaagaagaacatattcttccctccctgcgcacccaaggacgtgaaccacgccgagttctaccgcgagtgcaggccgccctgctactactttgtgaccaaggactacgggcatctggacatgctggacgacgacgcccccaagttcatcacctgcgtctgcaaggatgggaacgggtgcaagggcaagatgcggaggtgcgttgctgggatcatggtggcatttcttaatgctgccttgggtgagaaagatgcagatcttgaggccatactgagagacccggcggttgcacccaccacgcttgatccggttgagcaccgcgtggcg BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_K208010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z