LOCUS       BBa_K1418012            1754 bp    DNA     linear       23-Dec-2024
DEFINITION  Cellulase (cenA) generator with 10x His-tag
ACCESSION   BBa_K1418012
VERSION     BBa_K1418012.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=atg
     CDS             230..1573
                     /label=cenA
     CDS             227..1573
                     /label=BBa_K1418011
     misc_feature    1582..1611
                     /label=10x-Histidine Tag
     misc_feature    1612..1614
                     /label=Stop
     misc_feature    1615..1617
                     /label=Stop
     misc_feature    1582..1617
                     /label=BBa_K844000
     stem_loop       1637..1680
                     /label=stem_loop
     misc_feature    1626..1705
                     /label=BBa_B0010
     terminator      1626..1705
                     /label=BBa_B0010
     stem_loop       1721..1740
                     /label=T7 TE
     misc_feature    1747..1747
                     /label=stop
     polyA_site      1741..1754
                     /label=polya
     misc_feature    1714..1754
                     /label=BBa_B0012
     terminator      1714..1754
                     /label=BBa_B0012
     terminator      1626..1754
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccacccgcag
      241 aaccgccgca gcgctgctgg cggccgcggc cgtcgccgtc ggcggtctga ccgccctcac
      301 caccaccgcc gcgcaggcgg ctcccggctg ccgcgtcgac tacgccgtca ccaaccagtg
      361 gcccggcggc ttcggcgcca acgtcacgat caccaacctc ggcgaccccg tctcgtcgtg
      421 gaagctcgac tggacctaca ccgcaggcca gcggatccag cagctgtgga acggcaccgc
      481 gtcgaccaac ggcggccagg tctccgtcac cagcctgccc tggaacggca gcatcccgac
      541 cggcggcacg gcgtcgttcg ggttcaacgg ctcgtgggcc gggtccaacc cgacgccggc
      601 gtcgttctcg ctcaacggca ccacctgcac gggcaccgtg ccgacgacca gccccacgcc
      661 gaccccgacg ccgacgaccc ccacgccgac gccgaccccg acccccaccc ccacgccgac
      721 ggtcacgccg cagccgacca gcggcttcta cgtcgacccg acgacgcagg gctaccgcgc
      781 gtggcaggcc gcgtccggca cggacaaggc gctgctcgag aagatcgcgc tcaccccgca
      841 ggcgtactgg gtcggcaact gggccgacgc gtcgcacgcg caggccgagg tcgccgacta
      901 caccggccgc gccgtcgcgg ccgggaagac gccgatgctc gtcgtctacg cgatcccggg
      961 ccgcgactgc ggctcgcact ccggcggtgg tgtgtccgag tccgagtacg cgcgctgggt
     1021 cgacaccgtc gcgcagggca tcaagggcaa cccgatcgtg atcctcgagc ccgacgcgct
     1081 cgcgcagctc ggcgactgct ccggccaggg tgaccgcgtc ggcttcctca agtacgccgc
     1141 caagtcgctc accctcaagg gcgcgcgcgt ctacatcgac gcgggccacg cgaagtggct
     1201 gtcggtcgac acgccggtga accgcctcaa ccaggtcggc ttcgagtacg cggtgggctt
     1261 cgcgctcaac acgtcgaact accagacgac ggcggacagc aaggcgtacg gccagcagat
     1321 ctcgcagcgg ctgggcggca agaagttcgt catcgacacc tcgcgcaacg gcaacggctc
     1381 gaacggcgag tggtgcaacc cgcgcggccg cgcgctcggc gaacgcccgg tcgcggtgaa
     1441 cgacggctcc ggcctggacg cgctcctgtg ggtcaagctg cccggcgagt ccgacggcgc
     1501 gtgcaacggc ggcccggccg ccggccagtg gtggcaggag atcgccctgg agatggcgcg
     1561 caacgccagg tggtactaga gcatcatcac catcaccacc atcatcacca ttaataatac
     1621 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1681 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1741 tttctgcgtt tata
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