LOCUS       BBa_K1418022            2435 bp    DNA     linear       23-Dec-2024
DEFINITION  p-amylase(ns)HTB0015
ACCESSION   BBa_K1418022
VERSION     BBa_K1418022.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=atg
     CDS             230..2254
                     /label=alpha-amylase
     CDS             227..2254
                     /label=BBa_K1418021
     misc_feature    2263..2292
                     /label=10x-Histidine Tag
     misc_feature    2293..2295
                     /label=Stop
     misc_feature    2296..2298
                     /label=Stop
     misc_feature    2263..2298
                     /label=BBa_K844000
     stem_loop       2318..2361
                     /label=stem_loop
     misc_feature    2307..2386
                     /label=BBa_B0010
     terminator      2307..2386
                     /label=BBa_B0010
     stem_loop       2402..2421
                     /label=T7 TE
     misc_feature    2428..2428
                     /label=stop
     polyA_site      2422..2435
                     /label=polya
     misc_feature    2395..2435
                     /label=BBa_B0012
     terminator      2395..2435
                     /label=BBa_B0012
     terminator      2307..2435
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aactcgccgc
      241 ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt
      301 tcccgccttt agcgaacagg ggacaggaac atttgtcagc cacgcgcagt tgcccaaagg
      361 tacgcgtcca ctaacgctaa attttgacca acagtgctgg cagcctgcgg atgcgataaa
      421 actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt
      481 cagggacggc gaatatacgc tgcaaataga cacccgctcc ggtacgccaa cattgatgat
      541 ttccatccag aacgccgccg aaccggtagc aagcctggtc cgtgaatgcc cgaaatggga
      601 tggattaccg ctcacagtgg atgtcagcgc cactttcccg gaaggagccg ccgtacggga
      661 ttattacagc cagcaaattg cgatagtgaa gaacggtcaa ataatgttac aacccgctgc
      721 caccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg cccctttcga
      781 ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag
      841 taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg
      901 cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg gcgttaatgc
      961 tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg
     1021 cgatttcccg cattatgcct accacggtta ttacacacag gactggacga atcttgatgc
     1081 caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc agcgcggtat
     1141 tcgtattctc tttgatgtcg tgatgaacca caccggctat gccacgctgg cggatatgca
     1201 ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaat cgctgggtga
     1261 acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat
     1321 taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaacgga
     1381 tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctag cctttttgcc
     1441 ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata aaaacaaaat
     1501 ggatacccac gccaaagcca ttgacggcta tacgccgcgc gattacttaa cccactggtt
     1561 aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt
     1621 tgagttgccc gcctggcagc aactgaaaac cgaagccagc gccgcgcttc gcgaatggaa
     1681 aaaagctaac cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagcctg
     1741 gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt
     1801 cgattatcag gagcaggcgg cgaaagcagt cgactgtctg gcgcagatgg atacgacctg
     1861 gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct cgtcgcatga
     1921 tacccgcctg ttccgtgaag ggggcgacaa agcagcagag ttattactat tagcgccagg
     1981 cgcggtacaa atcttttatg gtgatgaatc ctcgcgtccg ttcggtccta caggttctga
     2041 tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc
     2101 cagcgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc
     2161 gggcaaacaa acgacacttt tgctgaagca gggctacggc tttgttcgtg agcatggcga
     2221 cgataaagtg ctggtcgtct gggcagggca acagtactag agcatcatca ccatcaccac
     2281 catcatcacc attaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2341 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2401 tggctcacct tcgggtgggc ctttctgcgt ttata
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