##gff-version 3
##sequence-region BBa_K1418022 1 2435
BBa_K1418022	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_K1418022	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_K1418022	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_K1418022	.	tag	2263	2298	.	+	0	ID=BBa_K844000;Name=BBa_K844000
BBa_K1418022	.	stop_codon	2296	2298	.	+	0	ID=annotation2206609;Name=Stop;Parent=BBa_K844000
BBa_K1418022	.	stop_codon	2293	2295	.	+	0	ID=annotation2206608;Name=Stop;Parent=BBa_K844000
BBa_K1418022	.	sequence_feature	2263	2292	.	+	0	ID=annotation2206607;Name=10x-Histidine Tag;Parent=BBa_K844000
BBa_K1418022	.	CDS	227	2254	.	+	0	ID=BBa_K1418021;Name=BBa_K1418021
BBa_K1418022	.	CDS	230	2254	.	+	0	ID=annotation2421281;Name=alpha-amylase;Parent=BBa_K1418021
BBa_K1418022	.	start_codon	227	229	.	+	0	ID=annotation2421280;Name=atg;Parent=BBa_K1418021
BBa_K1418022	.	terminator	2307	2435	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1418022	.	terminator	2395	2435	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1418022	.	stem_loop	2402	2421	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1418022	.	stop_codon	2428	2428	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1418022	.	polyA_site	2422	2435	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1418022	.	engineered_region	2395	2435	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1418022	.	terminator	2307	2386	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1418022	.	stem_loop	2318	2361	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1418022	.	engineered_region	2307	2386	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1418022
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaactcgc
cgcctgttttctgacactccttcctggcttcgccgttgccgccagctggacttctccggggtttcccgcctttagcgaa
caggggacaggaacatttgtcagccacgcgcagttgcccaaaggtacgcgtccactaacgctaaattttgaccaacagt
gctggcagcctgcggatgcgataaaactcaatcagatgctttccctgcaaccttgtagcaacacgccgcctcaatggcg
attgttcagggacggcgaatatacgctgcaaatagacacccgctccggtacgccaacattgatgatttccatccagaac
gccgccgaaccggtagcaagcctggtccgtgaatgcccgaaatgggatggattaccgctcacagtggatgtcagcgcca
ctttcccggaaggagccgccgtacgggattattacagccagcaaattgcgatagtgaagaacggtcaaataatgttaca
acccgctgccaccagcaacggtttactcctgctggaacgggcagaaactgacacatccgcccctttcgactggcataac
gccacggtttactttgtgctgacagatcgtttcgaaaacggcgatcccagtaatgaccagagttacggacgtcataaag
acggtatggcggaaattggcacttttcacggcggcgatttacgcggcctgaccaacaaactggattacctccagcagtt
gggcgttaatgctttatggataagcgccccatttgagcaaattcacggctgggtcggcggcggtacaaaaggcgatttc
ccgcattatgcctaccacggttattacacacaggactggacgaatcttgatgccaatatgggcaacgaagccgatctac
ggacgctggttgatagcgcacatcagcgcggtattcgtattctctttgatgtcgtgatgaaccacaccggctatgccac
gctggcggatatgcaggagtatcagtttggcgcgttatatctttctggtgacgaagtgaaaaaatcgctgggtgaacgc
tggagcgactggaaacctgccgccgggcaaacctggcatagctttaacgattacattaatttcagcgacaaaacaggct
gggataaatggtggggaaaaaactggatcagaacggatatcggcgattacgacaatcctggattcgacgatctcactat
gtcgctagcctttttgccggatatcaaaaccgaatcaactaccgcttctggtctgccggtgttctataaaaacaaaatg
gatacccacgccaaagccattgacggctatacgccgcgcgattacttaacccactggttaagtcagtgggtccgcgact
atgggattgatggttttcgggtcgataccgccaaacatgttgagttgcccgcctggcagcaactgaaaaccgaagccag
cgccgcgcttcgcgaatggaaaaaagctaaccccgacaaagcattagatgacaaacctttctggatgaccggtgaagcc
tggggccacggcgtgatgcaaagtgactactatcgccacggcttcgatgcgatgatcaatttcgattatcaggagcagg
cggcgaaagcagtcgactgtctggcgcagatggatacgacctggcagcaaatggcggagaaattgcagggtttcaacgt
gttgagctacctctcgtcgcatgatacccgcctgttccgtgaagggggcgacaaagcagcagagttattactattagcg
ccaggcgcggtacaaatcttttatggtgatgaatcctcgcgtccgttcggtcctacaggttctgatccgctgcaaggta
cacgttcggatatgaactggcaggatgttagcggtaaatctgccgccagcgtcgcgcactggcagaaaatcagccagtt
ccgcgcccgccatcccgcaattggcgcgggcaaacaaacgacacttttgctgaagcagggctacggctttgttcgtgag
catggcgacgataaagtgctggtcgtctgggcagggcaacagtactagagcatcatcaccatcaccaccatcatcacca
ttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata