LOCUS BBa_K1418050 4436 bp DNA linear 23-Dec-2024 DEFINITION Inducible PHB generator ACCESSION BBa_K1418050 VERSION BBa_K1418050.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 CDS 581..2350 /label=phaC1 CDS 2435..3616 /label=phaA CDS 3691..4431 /label=phaB1 promoter 229..278 /label=Pwt RBS 564..570 /label=RBS RBS 2424..2427 /label=RBS RBS 3681..3684 /label=RBS misc_feature 229..4436 /label=BBa_K934001 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagga ttcccgcatt 241 gacagcgcgt gcgttgcaag gcaacaatgg actcaaatgt ctcggaatcg ctgacgattc 301 ccaggtttct ccggcaagca tagcgcatgg cgtctccatg cgagaatgtc gcgcttgccg 361 gataaaaggg gagccgctat cggaatggac gcaagccacg gccgcagcag gtgcggtcga 421 gggcttccag ccagttccag ggcagatgtg ccggcagacc ctcccgcttt gggggaggcg 481 caagccgggt ccattcggat agcatctccc catgcaaagt gccggccagg gcaatgcccg 541 gagccggttc gaatagtgac ggcagagaga caatcaaatc atggctactg ggaaaggagc 601 cgccgccagc acccaggaag gcaaaagcca accatttaaa gtgaccccag gcccgttcga 661 tcctgccacc tggctggaat ggtcacgtca atggcaaggt acggagggca acggccatgc 721 ggcagcgtcc ggcatccctg ggcttgatgc actggctggt gttaaaattg cgcctgccca 781 attgggagac atccaacagc gttatatgaa agatttttcg gcgctgtggc aggcgatggc 841 ggaaggtaaa gcagaggcga cgggaccgct gcatgaccgt cgctttgctg gcgacgcctg 901 gcgtaccaat ttgccctatc gttttgcggc agccttctac ctgctgaacg ctcgtgccct 961 gactgagctg gcagatgctg tcgaagcgga tgccaaaacc cgccaacgca tccgttttgc 1021 catctcacag tgggtcgacg caatgtcacc ggccaacttc ctggccacca atccggaagc 1081 acagcgcctc ctgattgaaa gcggcgggga atcgctgcgc gccggcgttc gtaacatgat 1141 ggaagatctg acccgtggta agatttcgca gacggatgag tcggccttcg aggtgggtcg 1201 caatgttgca gtgacagaag gcgcggtggt gtttgagaac gaatactttc aacttctcca 1261 gtataaacca ctgacggata aagtgcatgc gcgcccattg ttaatggttc ccccgtgcat 1321 taataaatac tatatcctgg atcttcagcc ggaaagctcc ttagtgcgcc acgtggtgga 1381 acaggggcat actgtgtttt tagttagctg gcgcaatcca gatgcctcaa tggccggcag 1441 tacctgggat gactacattg aacacgcggc tattcgcgcg atcgaagtgg cacgcgacat 1501 ctcaggtcag gacaagatta acgtactcgg cttttgcgtc ggtggcacta tcgtgagcac 1561 cgctctggcg gtactggctg cgcgtggtga acaccccgca gcgtcggtta cgcttctgac 1621 cacactgttg gatttcgccg acactggcat cttggatgta tttgtcgatg agggccacgt 1681 tcagctgcgc gaggccactc tgggtggcgg cgccggcgca ccgtgcgctc tgctgcgcgg 1741 tctggagctc gcgaatactt tttctttctt acgcccgaac gatcttgtct ggaattacgt 1801 agttgacaac tatctcaaag gtaacacgcc cgtgccgttt gatttattat tctggaatgg 1861 ggatgcgacc aacctgccgg gtccttggta ttgttggtat ctccgtcata catacttaca 1921 gaatgaatta aaggtcccag gtaaacttac tgtgtgtggt gtcccggtag atctggcgag 1981 tatcgacgtg ccgacctaca tttatgggag tcgtgaagat catattgtgc cgtggaccgc 2041 cgcgtacgct tctaccgcgc ttctggcaaa caaattacgt ttcgttctgg gtgccagcgg 2101 ccacattgcg ggtgttatta atcctccggc caaaaataaa cgctcgcatt ggacaaacga 2161 cgccttgccg gagagccctc agcagtggtt ggcgggtgcc attgagcacc atggaagttg 2221 gtggccggac tggacagcct ggctggcggg ccaagcaggc gcaaaacgcg ctgcgccagc 2281 caactatggc aacgcgcgct accgcgcgat tgaacccgcg cctggccgct atgtaaaagc 2341 aaaagcgtga cgcttgcatg agtgccggcg tgcgtcatgc acggcgccgg caggcctgca 2401 cgttccctcc cgtttccatt gaaaggacta cacaatgacc gatgttgtta tcgtcagtgc 2461 cgcgcgcacc gccgtgggta aatttggcgg cagtctggcg aaaatcccgg cgccagaatt 2521 gggcgcggtg gtaatcaaag cggccctgga acgcgccggt gtgaaacctg agcaggtgag 2581 cgaagtaatt atgggtcagg ttctgaccgc gggcagcggg caaaatccgg cgcgtcaggc 2641 tgcgattaag gcaggccttc cggcgatggt gcccgcgatg accattaaca aagtatgcgg 2701 atcgggactg aaagcagtga tgctggccgc taacgccatt atggcaggcg acgcggaaat 2761 tgtggtcgct ggcggacagg agaatatgtc agccgcgcct catgtgctgc cgggtagccg 2821 cgacggcttt cgcatgggtg atgcaaaact ggtcgatact atgattgttg acggcttatg 2881 ggatgtgtac aaccagtacc acatgggcat caccgccgag aacgtggcca aggaatacgg 2941 catcacacgc gaggcgcagg atgagttcgc cgtcggctcg cagaacaagg ccgaagccgc 3001 gcagaaggcc ggcatgtttg acgaagagat cgtcccggtg ctgatcccgc agcgcaaggg 3061 cgacccggtg gccttcaaga ccgacgagtt cgtgcgccag ggcgccacgc tggacagcat 3121 gtccggcctc aagcccgcct tcgacaaggc cggcacggtg accgcggcca acgcctcggg 3181 cctgaacgac ggcgccgccg cggtggtggt gatgtcggcg gccaaggcca aggaactggg 3241 cctgaccccg ctggccacga tcaagagcta tgccaacgcc ggtgtcgatc ccaaggtgat 3301 gggcatgggc ccggtgccgg cctccaagcg cgccctgtcg cgcgccgagt ggaccccgca 3361 agacctggac ctgatggaga tcaacgaggc ctttgccgcg caggcgctgg cggtgcacca 3421 gcagatgggc tgggacacct ccaaggtcaa tgtgaacggc ggcgccatcg ccatcggcca 3481 cccgatcggc gcgtcgggct gccgtatcct ggtgacgctg ctgcacgaga tgaagcgccg 3541 tgacgcgaag aagggcctgg cctcgctgtg catcggcggc ggcatgggcg tggcgctggc 3601 agtcgagcgc aaataaggaa ggggttttcc ggggccgcgc gcggttggcg cggacccggc 3661 gacgataacg aagccaatca aggagtggac atgactcagc gcattgcgta tgtgaccggc 3721 ggcatgggtg gtatcggaac cgccatttgc cagcggctgg ccaaggatgg ctttcgtgtg 3781 gtggccggtt gcggccccaa ctcgccgcgc cgcgaaaagt ggctggagca gcagaaggcc 3841 ctgggcttcg atttcattgc ctcggaaggc aatgtggctg actgggactc gaccaagacc 3901 gcattcgaca aggtcaagtc cgaggtcggc gaggttgatg tgctgatcaa caacgccggt 3961 atcacccgcg acgtggtgtt ccgcaagatg acccgcgccg actgggatgc ggtgatcgac 4021 accaacctga cctcgctgtt caacgtcacc aagcaggtga tcgacggcat ggccgaccgt 4081 ggctggggcc gcatcgtcaa catctcgtcg gtgaacgggc agaagggcca gttcggccag 4141 accaactact ccaccgccaa ggccggcctg catggcttca ccatggcact ggcgcaggaa 4201 gtggcgacca agggcgtgac cgtcaacacg gtctctccgg gctatatcgc caccgacatg 4261 gtcaaggcga tccgccagga cgtgctcgac aagatcgtcg cgacgatccc ggtcaagcgc 4321 ctgggcctgc cggaagagat cgcctcgatc tgcgcctggt tgtcgtcgga ggagtccggt 4381 ttctcgaccg gcgccgactt ctcgctcaac ggcggcctgc atatgggctg acctgc //