LOCUS BBa_K1418061 7093 bp DNA linear 06-Dec-2025 DEFINITION phaCAB-p-GFPPhaC-B0015 ACCESSION BBa_K1418061 VERSION BBa_K1418061.1 FEATURES Location/Qualifiers CDS 353..2122 /label=phaC1 CDS 2207..3388 /label=phaA CDS 3463..4203 /label=phaB1 promoter 1..50 /label=Pwt RBS 336..342 /label=RBS RBS 2196..2199 /label=RBS RBS 3453..3456 /label=RBS misc_feature 1..4208 /label=BBa_K934001 CDS 4217..4304 /label=end of LacI coding region (inactive) misc_feature 4217..4416 /label=BBa_R0010 misc_feature 4305..4342 /label=CAP binding site promoter 4353..4358 /label=-35 promoter 4377..4382 /label=-10 misc_feature 4382..4416 /label=LacI binding site misc_feature 4389..4389 /label=start promoter 4217..4416 /label=BBa_R0010 misc_feature 4429..4432 /label=conserved RBS 4425..4436 /label=BBa_B0034 misc_feature 4443..4445 /label=start misc_feature 4865..4870 /label=XhoI removed CDS 4443..5159 /label=Coding CDS 4443..5159 /label=BBa_K1418062 misc_feature 5181..5183 /label=atg CDS 5184..6947 /label=phaC misc_feature 6951..6956 /label=taataa CDS 5168..6956 /label=BBa_K1418060 stem_loop 6976..7019 /label=stem_loop misc_feature 6965..7044 /label=BBa_B0010 terminator 6965..7044 /label=BBa_B0010 stem_loop 7060..7079 /label=T7 TE misc_feature 7086..7086 /label=stop polyA_site 7080..7093 /label=polya misc_feature 7053..7093 /label=BBa_B0012 terminator 7053..7093 /label=BBa_B0012 terminator 6965..7093 /label=BBa_B0015 ORIGIN 1 gattcccgca ttgacagcgc gtgcgttgca aggcaacaat ggactcaaat gtctcggaat 61 cgctgacgat tcccaggttt ctccggcaag catagcgcat ggcgtctcca tgcgagaatg 121 tcgcgcttgc cggataaaag gggagccgct atcggaatgg acgcaagcca cggccgcagc 181 aggtgcggtc gagggcttcc agccagttcc agggcagatg tgccggcaga ccctcccgct 241 ttgggggagg cgcaagccgg gtccattcgg atagcatctc cccatgcaaa gtgccggcca 301 gggcaatgcc cggagccggt tcgaatagtg acggcagaga gacaatcaaa tcatggctac 361 tgggaaagga gccgccgcca gcacccagga aggcaaaagc caaccattta aagtgacccc 421 aggcccgttc gatcctgcca cctggctgga atggtcacgt caatggcaag gtacggaggg 481 caacggccat gcggcagcgt ccggcatccc tgggcttgat gcactggctg gtgttaaaat 541 tgcgcctgcc caattgggag acatccaaca gcgttatatg aaagattttt cggcgctgtg 601 gcaggcgatg gcggaaggta aagcagaggc gacgggaccg ctgcatgacc gtcgctttgc 661 tggcgacgcc tggcgtacca atttgcccta tcgttttgcg gcagccttct acctgctgaa 721 cgctcgtgcc ctgactgagc tggcagatgc tgtcgaagcg gatgccaaaa cccgccaacg 781 catccgtttt gccatctcac agtgggtcga cgcaatgtca ccggccaact tcctggccac 841 caatccggaa gcacagcgcc tcctgattga aagcggcggg gaatcgctgc gcgccggcgt 901 tcgtaacatg atggaagatc tgacccgtgg taagatttcg cagacggatg agtcggcctt 961 cgaggtgggt cgcaatgttg cagtgacaga aggcgcggtg gtgtttgaga acgaatactt 1021 tcaacttctc cagtataaac cactgacgga taaagtgcat gcgcgcccat tgttaatggt 1081 tcccccgtgc attaataaat actatatcct ggatcttcag ccggaaagct ccttagtgcg 1141 ccacgtggtg gaacaggggc atactgtgtt tttagttagc tggcgcaatc cagatgcctc 1201 aatggccggc agtacctggg atgactacat tgaacacgcg gctattcgcg cgatcgaagt 1261 ggcacgcgac atctcaggtc aggacaagat taacgtactc ggcttttgcg tcggtggcac 1321 tatcgtgagc accgctctgg cggtactggc tgcgcgtggt gaacaccccg cagcgtcggt 1381 tacgcttctg accacactgt tggatttcgc cgacactggc atcttggatg tatttgtcga 1441 tgagggccac gttcagctgc gcgaggccac tctgggtggc ggcgccggcg caccgtgcgc 1501 tctgctgcgc ggtctggagc tcgcgaatac tttttctttc ttacgcccga acgatcttgt 1561 ctggaattac gtagttgaca actatctcaa aggtaacacg cccgtgccgt ttgatttatt 1621 attctggaat ggggatgcga ccaacctgcc gggtccttgg tattgttggt atctccgtca 1681 tacatactta cagaatgaat taaaggtccc aggtaaactt actgtgtgtg gtgtcccggt 1741 agatctggcg agtatcgacg tgccgaccta catttatggg agtcgtgaag atcatattgt 1801 gccgtggacc gccgcgtacg cttctaccgc gcttctggca aacaaattac gtttcgttct 1861 gggtgccagc ggccacattg cgggtgttat taatcctccg gccaaaaata aacgctcgca 1921 ttggacaaac gacgccttgc cggagagccc tcagcagtgg ttggcgggtg ccattgagca 1981 ccatggaagt tggtggccgg actggacagc ctggctggcg ggccaagcag gcgcaaaacg 2041 cgctgcgcca gccaactatg gcaacgcgcg ctaccgcgcg attgaacccg cgcctggccg 2101 ctatgtaaaa gcaaaagcgt gacgcttgca tgagtgccgg cgtgcgtcat gcacggcgcc 2161 ggcaggcctg cacgttccct cccgtttcca ttgaaaggac tacacaatga ccgatgttgt 2221 tatcgtcagt gccgcgcgca ccgccgtggg taaatttggc ggcagtctgg cgaaaatccc 2281 ggcgccagaa ttgggcgcgg tggtaatcaa agcggccctg gaacgcgccg gtgtgaaacc 2341 tgagcaggtg agcgaagtaa ttatgggtca ggttctgacc gcgggcagcg ggcaaaatcc 2401 ggcgcgtcag gctgcgatta aggcaggcct tccggcgatg gtgcccgcga tgaccattaa 2461 caaagtatgc ggatcgggac tgaaagcagt gatgctggcc gctaacgcca ttatggcagg 2521 cgacgcggaa attgtggtcg ctggcggaca ggagaatatg tcagccgcgc ctcatgtgct 2581 gccgggtagc cgcgacggct ttcgcatggg tgatgcaaaa ctggtcgata ctatgattgt 2641 tgacggctta tgggatgtgt acaaccagta ccacatgggc atcaccgccg agaacgtggc 2701 caaggaatac ggcatcacac gcgaggcgca ggatgagttc gccgtcggct cgcagaacaa 2761 ggccgaagcc gcgcagaagg ccggcatgtt tgacgaagag atcgtcccgg tgctgatccc 2821 gcagcgcaag ggcgacccgg tggccttcaa gaccgacgag ttcgtgcgcc agggcgccac 2881 gctggacagc atgtccggcc tcaagcccgc cttcgacaag gccggcacgg tgaccgcggc 2941 caacgcctcg ggcctgaacg acggcgccgc cgcggtggtg gtgatgtcgg cggccaaggc 3001 caaggaactg ggcctgaccc cgctggccac gatcaagagc tatgccaacg ccggtgtcga 3061 tcccaaggtg atgggcatgg gcccggtgcc ggcctccaag cgcgccctgt cgcgcgccga 3121 gtggaccccg caagacctgg acctgatgga gatcaacgag gcctttgccg cgcaggcgct 3181 ggcggtgcac cagcagatgg gctgggacac ctccaaggtc aatgtgaacg gcggcgccat 3241 cgccatcggc cacccgatcg gcgcgtcggg ctgccgtatc ctggtgacgc tgctgcacga 3301 gatgaagcgc cgtgacgcga agaagggcct ggcctcgctg tgcatcggcg gcggcatggg 3361 cgtggcgctg gcagtcgagc gcaaataagg aaggggtttt ccggggccgc gcgcggttgg 3421 cgcggacccg gcgacgataa cgaagccaat caaggagtgg acatgactca gcgcattgcg 3481 tatgtgaccg gcggcatggg tggtatcgga accgccattt gccagcggct ggccaaggat 3541 ggctttcgtg tggtggccgg ttgcggcccc aactcgccgc gccgcgaaaa gtggctggag 3601 cagcagaagg ccctgggctt cgatttcatt gcctcggaag gcaatgtggc tgactgggac 3661 tcgaccaaga ccgcattcga caaggtcaag tccgaggtcg gcgaggttga tgtgctgatc 3721 aacaacgccg gtatcacccg cgacgtggtg ttccgcaaga tgacccgcgc cgactgggat 3781 gcggtgatcg acaccaacct gacctcgctg ttcaacgtca ccaagcaggt gatcgacggc 3841 atggccgacc gtggctgggg ccgcatcgtc aacatctcgt cggtgaacgg gcagaagggc 3901 cagttcggcc agaccaacta ctccaccgcc aaggccggcc tgcatggctt caccatggca 3961 ctggcgcagg aagtggcgac caagggcgtg accgtcaaca cggtctctcc gggctatatc 4021 gccaccgaca tggtcaaggc gatccgccag gacgtgctcg acaagatcgt cgcgacgatc 4081 ccggtcaagc gcctgggcct gccggaagag atcgcctcga tctgcgcctg gttgtcgtcg 4141 gaggagtccg gtttctcgac cggcgccgac ttctcgctca acggcggcct gcatatgggc 4201 tgacctgcta ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 4381 ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact 4441 agatggctag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag 4501 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgctacat 4561 acggaaagct tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa 4621 cacttgtcac tactttctct tatggtgttc aatgcttttc ccgttatccg gatcatatga 4681 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat 4741 ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa ggtgataccc 4801 ttgttaatcg tatcgagtta aaaggtattg attttaaaga agatggaaac attctcggac 4861 acaaacttga gtacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga 4921 atggaatcaa agctaacttc aaaattcgcc acaacattga agatggatcc gttcaactag 4981 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc 5041 attacctgtc gacacaatct gccctttcga aagatcccaa cgaaaagcgt gaccacatgg 5101 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat 5161 actagagaag gaggtatata atggcgaccg gcaaaggcgc ggcagcttcc acgcaggaag 5221 gcaagtccca accattcaag gtcacgccgg ggccattcga tccagccaca tggctggaat 5281 ggtcccgcca gtggcagggc actgaaggca acggccacgc gaccgcgtcc ggcattccgg 5341 gcctggatgc gctggcaggc gtcaagatcg cgccggcgca gctgggtgat atccagcagc 5401 gctacatgaa ggacttctca gcgctgtggc aggccatggc cgagggcaag gccgaggcca 5461 ccggtccgct gcacgaccgg cgcttcgccg gcgacgcatg gcgcaccaac ctcccatatc 5521 gcttcgctgc cgcgttctac ctgctcaatg cgcgcgcctt gaccgagctg gccgatgccg 5581 tcgaggccga tgccaagacc cgccagcgca tccgcttcgc gatctcgcaa tgggtcgatg 5641 cgatgtcgcc cgccaacttc cttgccacca atcccgaggc gcagcgcctg ctgatcgagt 5701 cgggcggcga atcgctgcgt gccggcgtgc gcaacatgat ggaagacctg acacgcggca 5761 agatctcgca gaccgacgag agcgcgtttg aggtcggccg caatgtcgcg gtgaccgaag 5821 gcgccgtggt cttcgagaac gagtacttcc agctgttgca gtacaagccg ctgaccgaca 5881 aggtgcacgc gcgcccgctg ctgatggtgc cgccgtgcat caacaagtac tacatcctgg 5941 acatgcagcc ggagagctcg ctggtgcgcc atgtggtgga gcagggacat acggtgtttc 6001 tggtgtcgtg gcgcaatccg gacgccagca tggccggcag cacctgggac gactacatcg 6061 agcacgcggc catccgcgcc atcgaagtcg cgcgcgacat cagcggccag gacaagatca 6121 acgtgctcgg cttctgcgtg ggcggcacca ttgtctcgac cgcgctggcg gtgctggccg 6181 cgcgcggcga gcacccggcc gccagcgtca cgctgctgac cacgctgctg gactttgccg 6241 acacgggcat cctcgacgtc tttgtcgacg agggccatgt gcagttgcgc gaggccacgc 6301 tgggcggcgg cgccggcgcg ccgtgcgcgc tgctgcgcgg ccttgagctg gccaatacct 6361 tctcgttctt gcgcccgaac gacctggtgt ggaactacgt ggtcgacaac tacctgaagg 6421 gcaacacgcc ggtgccgttc gacctgctgt tctggaacgg cgacgccacc aacctgccgg 6481 ggccgtggta ctgctggtac ctgcgccaca cctacatgca gaacgagctc aaggtaccgg 6541 gcaagctgac cgtgtgcggc gtgccggtgg acctggccag catcgacgtg ccgacctata 6601 tctacggctc gcgcgaagac catatcgtgc cgtggaccgc ggcctatgcc tcgaccgcgc 6661 tgctggcgaa caagctgcgc ttcgtgctgg gtgcgtcggg ccatatcgcc ggtgtgatca 6721 acccgccggc caagaacaag cgcagccact ggactaacga tgcgctgccg gagtcgccgc 6781 agcaatggct ggccggcgcc atcgagcatc acggcagctg gtggccggac tggaccgcat 6841 ggctggccgg gcaggccggc gcgaaacgcg ccgcgcccgc caactatggc aatgcgcgct 6901 atcgcgcaat cgaacccgcg cctgggcgat acgtcaaagc caaggcatga taataatact 6961 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 7021 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 7081 ttctgcgttt ata //