##gff-version 3
##sequence-region BBa_K1418061 1 7093
BBa_K1418061	.	ribosome_entry_site	4425	4436	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1418061	.	conserved_region	4429	4432	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1418061	.	CDS	4443	5159	.	+	0	ID=BBa_K1418062;Name=BBa_K1418062
BBa_K1418061	.	sequence_alteration	4865	4870	.	+	0	ID=annotation2422056;Name=XhoI removed;Parent=BBa_K1418062
BBa_K1418061	.	start_codon	4443	4445	.	+	0	ID=annotation2422025;Name=start;Parent=BBa_K1418062
BBa_K1418061	.	CDS	4443	5159	.	+	0	ID=annotation2425830;Name=Coding;Parent=BBa_K1418062
BBa_K1418061	.	promoter	4217	4416	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1418061	.	non_covalent_binding_site	4382	4416	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1418061	.	non_covalent_binding_site	4305	4342	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1418061	.	promoter	4353	4358	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1418061	.	start_codon	4389	4389	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1418061	.	engineered_region	4217	4416	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1418061	.	CDS	4217	4304	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1418061	.	promoter	4377	4382	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1418061	.	CDS	5168	6956	.	+	0	ID=BBa_K1418060;Name=BBa_K1418060
BBa_K1418061	.	start_codon	5181	5183	.	+	0	ID=annotation2421445;Name=atg;Parent=BBa_K1418060
BBa_K1418061	.	CDS	5184	6947	.	+	0	ID=annotation2421446;Name=phaC;Parent=BBa_K1418060
BBa_K1418061	.	stop_codon	6951	6956	.	+	0	ID=annotation2421447;Name=taataa;Parent=BBa_K1418060
BBa_K1418061	.	engineered_region	1	4208	.	+	0	ID=BBa_K934001;Name=BBa_K934001
BBa_K1418061	.	promoter	1	50	.	+	0	ID=annotation2192328;Name=Pwt;Parent=BBa_K934001
BBa_K1418061	.	ribosome_entry_site	336	342	.	+	0	ID=annotation2192329;Name=RBS;Parent=BBa_K934001
BBa_K1418061	.	CDS	3463	4203	.	+	0	ID=annotation2192327;Name=phaB1;Parent=BBa_K934001
BBa_K1418061	.	CDS	2207	3388	.	+	0	ID=annotation2192326;Name=phaA;Parent=BBa_K934001
BBa_K1418061	.	CDS	353	2122	.	+	0	ID=annotation2192325;Name=phaC1;Parent=BBa_K934001
BBa_K1418061	.	ribosome_entry_site	3453	3456	.	+	0	ID=annotation2192331;Name=RBS;Parent=BBa_K934001
BBa_K1418061	.	ribosome_entry_site	2196	2199	.	+	0	ID=annotation2192330;Name=RBS;Parent=BBa_K934001
BBa_K1418061	.	terminator	6965	7093	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1418061	.	terminator	7053	7093	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1418061	.	polyA_site	7080	7093	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1418061	.	stop_codon	7086	7086	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1418061	.	stem_loop	7060	7079	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1418061	.	engineered_region	7053	7093	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1418061	.	terminator	6965	7044	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1418061	.	stem_loop	6976	7019	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1418061	.	engineered_region	6965	7044	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1418061
gattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtt
tctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaat
ggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctccc
gctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagc
cggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggca
aaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtac
ggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaa
ttgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcga
cgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttcta
cctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgtttt
gccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattg
aaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacgga
tgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaactt
ctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactata
tcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcg
caatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggca
cgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcgg
tactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcat
cttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgct
ctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgaca
actatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttg
gtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccg
gtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgt
acgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcc
tccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccatt
gagcaccatggaagttggtggccggactggacagcctggctggcgggccaagcaggcgcaaaacgcgctgcgccagcca
actatggcaacgcgcgctaccgcgcgattgaacccgcgcctggccgctatgtaaaagcaaaagcgtgacgcttgcatga
gtgccggcgtgcgtcatgcacggcgccggcaggcctgcacgttccctcccgtttccattgaaaggactacacaatgacc
gatgttgttatcgtcagtgccgcgcgcaccgccgtgggtaaatttggcggcagtctggcgaaaatcccggcgccagaat
tgggcgcggtggtaatcaaagcggccctggaacgcgccggtgtgaaacctgagcaggtgagcgaagtaattatgggtca
ggttctgaccgcgggcagcgggcaaaatccggcgcgtcaggctgcgattaaggcaggccttccggcgatggtgcccgcg
atgaccattaacaaagtatgcggatcgggactgaaagcagtgatgctggccgctaacgccattatggcaggcgacgcgg
aaattgtggtcgctggcggacaggagaatatgtcagccgcgcctcatgtgctgccgggtagccgcgacggctttcgcat
gggtgatgcaaaactggtcgatactatgattgttgacggcttatgggatgtgtacaaccagtaccacatgggcatcacc
gccgagaacgtggccaaggaatacggcatcacacgcgaggcgcaggatgagttcgccgtcggctcgcagaacaaggccg
aagccgcgcagaaggccggcatgtttgacgaagagatcgtcccggtgctgatcccgcagcgcaagggcgacccggtggc
cttcaagaccgacgagttcgtgcgccagggcgccacgctggacagcatgtccggcctcaagcccgccttcgacaaggcc
ggcacggtgaccgcggccaacgcctcgggcctgaacgacggcgccgccgcggtggtggtgatgtcggcggccaaggcca
aggaactgggcctgaccccgctggccacgatcaagagctatgccaacgccggtgtcgatcccaaggtgatgggcatggg
cccggtgccggcctccaagcgcgccctgtcgcgcgccgagtggaccccgcaagacctggacctgatggagatcaacgag
gcctttgccgcgcaggcgctggcggtgcaccagcagatgggctgggacacctccaaggtcaatgtgaacggcggcgcca
tcgccatcggccacccgatcggcgcgtcgggctgccgtatcctggtgacgctgctgcacgagatgaagcgccgtgacgc
gaagaagggcctggcctcgctgtgcatcggcggcggcatgggcgtggcgctggcagtcgagcgcaaataaggaaggggt
tttccggggccgcgcgcggttggcgcggacccggcgacgataacgaagccaatcaaggagtggacatgactcagcgcat
tgcgtatgtgaccggcggcatgggtggtatcggaaccgccatttgccagcggctggccaaggatggctttcgtgtggtg
gccggttgcggccccaactcgccgcgccgcgaaaagtggctggagcagcagaaggccctgggcttcgatttcattgcct
cggaaggcaatgtggctgactgggactcgaccaagaccgcattcgacaaggtcaagtccgaggtcggcgaggttgatgt
gctgatcaacaacgccggtatcacccgcgacgtggtgttccgcaagatgacccgcgccgactgggatgcggtgatcgac
accaacctgacctcgctgttcaacgtcaccaagcaggtgatcgacggcatggccgaccgtggctggggccgcatcgtca
acatctcgtcggtgaacgggcagaagggccagttcggccagaccaactactccaccgccaaggccggcctgcatggctt
caccatggcactggcgcaggaagtggcgaccaagggcgtgaccgtcaacacggtctctccgggctatatcgccaccgac
atggtcaaggcgatccgccaggacgtgctcgacaagatcgtcgcgacgatcccggtcaagcgcctgggcctgccggaag
agatcgcctcgatctgcgcctggttgtcgtcggaggagtccggtttctcgaccggcgccgacttctcgctcaacggcgg
cctgcatatgggctgacctgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagc
tggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcac
cccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagag
aaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcc
cgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatat
ctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagtt
aaaaggtattgattttaaagaagatggaaacattctcggacacaaacttgagtacaactataactcacacaatgtatac
atcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacaca
atctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattaca
catggcatggatgagctctacaaatactagagaaggaggtatataatggcgaccggcaaaggcgcggcagcttccacgc
aggaaggcaagtcccaaccattcaaggtcacgccggggccattcgatccagccacatggctggaatggtcccgccagtg
gcagggcactgaaggcaacggccacgcgaccgcgtccggcattccgggcctggatgcgctggcaggcgtcaagatcgcg
ccggcgcagctgggtgatatccagcagcgctacatgaaggacttctcagcgctgtggcaggccatggccgagggcaagg
ccgaggccaccggtccgctgcacgaccggcgcttcgccggcgacgcatggcgcaccaacctcccatatcgcttcgctgc
cgcgttctacctgctcaatgcgcgcgccttgaccgagctggccgatgccgtcgaggccgatgccaagacccgccagcgc
atccgcttcgcgatctcgcaatgggtcgatgcgatgtcgcccgccaacttccttgccaccaatcccgaggcgcagcgcc
tgctgatcgagtcgggcggcgaatcgctgcgtgccggcgtgcgcaacatgatggaagacctgacacgcggcaagatctc
gcagaccgacgagagcgcgtttgaggtcggccgcaatgtcgcggtgaccgaaggcgccgtggtcttcgagaacgagtac
ttccagctgttgcagtacaagccgctgaccgacaaggtgcacgcgcgcccgctgctgatggtgccgccgtgcatcaaca
agtactacatcctggacatgcagccggagagctcgctggtgcgccatgtggtggagcagggacatacggtgtttctggt
gtcgtggcgcaatccggacgccagcatggccggcagcacctgggacgactacatcgagcacgcggccatccgcgccatc
gaagtcgcgcgcgacatcagcggccaggacaagatcaacgtgctcggcttctgcgtgggcggcaccattgtctcgaccg
cgctggcggtgctggccgcgcgcggcgagcacccggccgccagcgtcacgctgctgaccacgctgctggactttgccga
cacgggcatcctcgacgtctttgtcgacgagggccatgtgcagttgcgcgaggccacgctgggcggcggcgccggcgcg
ccgtgcgcgctgctgcgcggccttgagctggccaataccttctcgttcttgcgcccgaacgacctggtgtggaactacg
tggtcgacaactacctgaagggcaacacgccggtgccgttcgacctgctgttctggaacggcgacgccaccaacctgcc
ggggccgtggtactgctggtacctgcgccacacctacatgcagaacgagctcaaggtaccgggcaagctgaccgtgtgc
ggcgtgccggtggacctggccagcatcgacgtgccgacctatatctacggctcgcgcgaagaccatatcgtgccgtgga
ccgcggcctatgcctcgaccgcgctgctggcgaacaagctgcgcttcgtgctgggtgcgtcgggccatatcgccggtgt
gatcaacccgccggccaagaacaagcgcagccactggactaacgatgcgctgccggagtcgccgcagcaatggctggcc
ggcgccatcgagcatcacggcagctggtggccggactggaccgcatggctggccgggcaggccggcgcgaaacgcgccg
cgcccgccaactatggcaatgcgcgctatcgcgcaatcgaacccgcgcctgggcgatacgtcaaagccaaggcatgata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata