BBa_K1418061
type: ComponentDefinition
persistent identity: BBa K1418061
display id: BBa_K1418061
version: 1
was generated by: iGEM to SBOL conversion
description: phaCAB-p-GFPPhaC-B0015
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:21Z
mutable provenance: Composite
owned by: james
owned by: myers
mutable description: Composite part of R0010, B0034, K1418062, K1418060, and B0015
discontinued: false
dominant: false
experience: experience/Works
group u list: _1796_
m user id: 0
owner id: 13965
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: GFPmut had stop codon removed to generate fusion protein
star: false
creator: Ryan Putman
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K934001
component: BBa_B0034
component: BBa_R0010
component: BBa_K1418060
component: BBa_K1418062
component: BBa_B0015
sequence annotation: BBa_K934001
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1418062
sequence annotation: BBa_K1418060
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1418061_sequence/1

BBa_K934001
type: Component
persistent identity: component2422318
display id: component2422318
version: 1
top level: BBa_K1418061
definition: phaC1-A-B1
access: public

BBa_B0034
type: Component
persistent identity: component2422327
display id: component2422327
version: 1
top level: BBa_K1418061
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2422319
display id: component2422319
version: 1
top level: BBa_K1418061
definition: LacI
access: public

BBa_K1418060
type: Component
persistent identity: component2422334
display id: component2422334
version: 1
top level: BBa_K1418061
definition: BBa_K1418060
access: public

BBa_K1418062
type: Component
persistent identity: component2422330
display id: component2422330
version: 1
top level: BBa_K1418061
definition: BBa_K1418062
access: public

BBa_B0015
type: Component
persistent identity: component2422341
display id: component2422341
version: 1
top level: BBa_K1418061
definition: BBa_B0015
access: public

BBa_K934001
type: SequenceAnnotation
persistent identity: annotation2422318
display id: annotation2422318
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422318/range2422318/1
component: BBa_K934001

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422318/range2422318/1
type: Range
persistent identity: range2422318
display id: range2422318
version: 1
top level: BBa_K1418061
direction: direction/reverse
start: 1
end: 4208
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2422319
display id: annotation2422319
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422319/range2422319/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422319/range2422319/1
type: Range
persistent identity: range2422319
display id: range2422319
version: 1
top level: BBa_K1418061
direction: direction/forward
start: 4217
end: 4416
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2422327
display id: annotation2422327
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422327/range2422327/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422327/range2422327/1
type: Range
persistent identity: range2422327
display id: range2422327
version: 1
top level: BBa_K1418061
direction: direction/reverse
start: 4425
end: 4436
orientation: inline

BBa_K1418062
type: SequenceAnnotation
persistent identity: annotation2422330
display id: annotation2422330
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422330/range2422330/1
component: BBa_K1418062

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422330/range2422330/1
type: Range
persistent identity: range2422330
display id: range2422330
version: 1
top level: BBa_K1418061
direction: direction/reverse
start: 4443
end: 5159
orientation: inline

BBa_K1418060
type: SequenceAnnotation
persistent identity: annotation2422334
display id: annotation2422334
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422334/range2422334/1
component: BBa_K1418060

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422334/range2422334/1
type: Range
persistent identity: range2422334
display id: range2422334
version: 1
top level: BBa_K1418061
direction: direction/reverse
start: 5168
end: 6956
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2422341
display id: annotation2422341
version: 1
top level: BBa_K1418061
location: https://synbiohub.org/public/igem/BBa_K1418061/annotation2422341/range2422341/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1418061/annotation2422341/range2422341/1
type: Range
persistent identity: range2422341
display id: range2422341
version: 1
top level: BBa_K1418061
direction: direction/reverse
start: 6965
end: 7093
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1418062
type: ComponentDefinition
persistent identity: BBa K1418062
display id: BBa_K1418062
version: 1
was generated by: iGEM to SBOL conversion
description: GFPmut
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:21Z
mutable provenance: GFPuv
owned by: james
owned by: myers
mutable description: GFPuv with XhoI site removed
discontinued: false
dominant: false
group u list: _1796_
m user id: 0
owner id: 13965
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Amino acid sequence was maintained after mutagenesis
star: false
creator: Ryan Putman
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Coding
sequence annotation: XhoI removed
sequence: https://synbiohub.org/public/igem/BBa_K1418062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2422025
display id: annotation2422025
version: 1
top level: BBa_K1418062
location: https://synbiohub.org/public/igem/BBa_K1418062/annotation2422025/range2422025/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1418062/annotation2422025/range2422025/1
type: Range
persistent identity: range2422025
display id: range2422025
version: 1
top level: BBa_K1418062
direction: direction/forward
start: 1
end: 3
orientation: inline

Coding
type: SequenceAnnotation
persistent identity: annotation2425830
display id: annotation2425830
version: 1
top level: BBa_K1418062
location: https://synbiohub.org/public/igem/BBa_K1418062/annotation2425830/range2425830/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1418062/annotation2425830/range2425830/1
type: Range
persistent identity: range2425830
display id: range2425830
version: 1
top level: BBa_K1418062
direction: direction/forward
start: 1
end: 717
orientation: inline

XhoI removed
type: SequenceAnnotation
persistent identity: annotation2422056
display id: annotation2422056
version: 1
top level: BBa_K1418062
location: https://synbiohub.org/public/igem/BBa_K1418062/annotation2422056/range2422056/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1418062/annotation2422056/range2422056/1
type: Range
persistent identity: range2422056
display id: range2422056
version: 1
top level: BBa_K1418062
direction: direction/forward
start: 423
end: 428
orientation: inline

BBa_K1418060
type: ComponentDefinition
persistent identity: BBa K1418060
display id: BBa_K1418060
version: 1
was generated by: iGEM to SBOL conversion
description: phaC
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:21Z
mutable provenance: phaC
owned by: james
owned by: myers
mutable description: phaC
discontinued: false
dominant: false
group u list: _1796_
m user id: 0
owner id: 13965
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFC 23
star: false
creator: Ryan Putman
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: atg
sequence annotation: phaC
sequence annotation: taataa
sequence: https://synbiohub.org/public/igem/BBa_K1418060_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421445
display id: annotation2421445
version: 1
top level: BBa_K1418060
location: https://synbiohub.org/public/igem/BBa_K1418060/annotation2421445/range2421445/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1418060/annotation2421445/range2421445/1
type: Range
persistent identity: range2421445
display id: range2421445
version: 1
top level: BBa_K1418060
direction: direction/forward
start: 14
end: 16
orientation: inline

phaC
type: SequenceAnnotation
persistent identity: annotation2421446
display id: annotation2421446
version: 1
top level: BBa_K1418060
location: https://synbiohub.org/public/igem/BBa_K1418060/annotation2421446/range2421446/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1418060/annotation2421446/range2421446/1
type: Range
persistent identity: range2421446
display id: range2421446
version: 1
top level: BBa_K1418060
direction: direction/forward
start: 17
end: 1780
orientation: inline

taataa
type: SequenceAnnotation
persistent identity: annotation2421447
display id: annotation2421447
version: 1
top level: BBa_K1418060
location: https://synbiohub.org/public/igem/BBa_K1418060/annotation2421447/range2421447/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1418060/annotation2421447/range2421447/1
type: Range
persistent identity: range2421447
display id: range2421447
version: 1
top level: BBa_K1418060
direction: direction/forward
start: 1784
end: 1789
orientation: inline

phaC1-A-B1
type: ComponentDefinition
persistent identity: BBa K934001
display id: BBa_K934001
version: 1
was generated by: iGEM to SBOL conversion
description: phaC1-A-B1 [P(3HB) synthesis]
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:47Z
mutable provenance: test
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1199_
m user id: 0
owner id: 14016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: test
star: false
creator: Taku Nakayama
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Pwt
sequence annotation: RBS
sequence annotation: phaC1
sequence annotation: phaA
sequence annotation: phaB1
sequence: https://synbiohub.org/public/igem/BBa_K934001_sequence/1

Pwt
type: SequenceAnnotation
persistent identity: annotation2192328
display id: annotation2192328
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K934001/annotation2192328/range2192328/1
type: Range
persistent identity: range2192328
display id: range2192328
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 1
end: 50
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192329
display id: annotation2192329
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K934001/annotation2192329/range2192329/1
type: Range
persistent identity: range2192329
display id: range2192329
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 336
end: 342
orientation: inline

phaC1
type: SequenceAnnotation
persistent identity: annotation2192325
display id: annotation2192325
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192325/range2192325/1
type: Range
persistent identity: range2192325
display id: range2192325
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 353
end: 2122
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192330
display id: annotation2192330
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K934001/annotation2192330/range2192330/1
type: Range
persistent identity: range2192330
display id: range2192330
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 2196
end: 2199
orientation: inline

phaA
type: SequenceAnnotation
persistent identity: annotation2192326
display id: annotation2192326
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192326/range2192326/1
type: Range
persistent identity: range2192326
display id: range2192326
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 2207
end: 3388
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2192331
display id: annotation2192331
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K934001/annotation2192331/range2192331/1
type: Range
persistent identity: range2192331
display id: range2192331
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 3453
end: 3456
orientation: inline

phaB1
type: SequenceAnnotation
persistent identity: annotation2192327
display id: annotation2192327
version: 1
top level: phaC1-A-B1
location: https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K934001/annotation2192327/range2192327/1
type: Range
persistent identity: range2192327
display id: range2192327
version: 1
top level: phaC1-A-B1
direction: direction/forward
start: 3463
end: 4203
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1418061_sequence/1
type: Sequence
persistent identity: BBa K1418061 sequence
display id: BBa_K1418061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1418062_sequence/1
type: Sequence
persistent identity: BBa K1418062 sequence
display id: BBa_K1418062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaacttgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1418060_sequence/1
type: Sequence
persistent identity: BBa K1418060 sequence
display id: BBa_K1418060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaggtatataatggcgaccggcaaaggcgcggcagcttccacgcaggaaggcaagtcccaaccattcaaggtcacgccggggccattcgatccagccacatggctggaatggtcccgccagtggcagggcactgaaggcaacggccacgcgaccgcgtccggcattccgggcctggatgcgctggcaggcgtcaagatcgcgccggcgcagctgggtgatatccagcagcgctacatgaaggacttctcagcgctgtggcaggccatggccgagggcaaggccgaggccaccggtccgctgcacgaccggcgcttcgccggcgacgcatggcgcaccaacctcccatatcgcttcgctgccgcgttctacctgctcaatgcgcgcgccttgaccgagctggccgatgccgtcgaggccgatgccaagacccgccagcgcatccgcttcgcgatctcgcaatgggtcgatgcgatgtcgcccgccaacttccttgccaccaatcccgaggcgcagcgcctgctgatcgagtcgggcggcgaatcgctgcgtgccggcgtgcgcaacatgatggaagacctgacacgcggcaagatctcgcagaccgacgagagcgcgtttgaggtcggccgcaatgtcgcggtgaccgaaggcgccgtggtcttcgagaacgagtacttccagctgttgcagtacaagccgctgaccgacaaggtgcacgcgcgcccgctgctgatggtgccgccgtgcatcaacaagtactacatcctggacatgcagccggagagctcgctggtgcgccatgtggtggagcagggacatacggtgtttctggtgtcgtggcgcaatccggacgccagcatggccggcagcacctgggacgactacatcgagcacgcggccatccgcgccatcgaagtcgcgcgcgacatcagcggccaggacaagatcaacgtgctcggcttctgcgtgggcggcaccattgtctcgaccgcgctggcggtgctggccgcgcgcggcgagcacccggccgccagcgtcacgctgctgaccacgctgctggactttgccgacacgggcatcctcgacgtctttgtcgacgagggccatgtgcagttgcgcgaggccacgctgggcggcggcgccggcgcgccgtgcgcgctgctgcgcggccttgagctggccaataccttctcgttcttgcgcccgaacgacctggtgtggaactacgtggtcgacaactacctgaagggcaacacgccggtgccgttcgacctgctgttctggaacggcgacgccaccaacctgccggggccgtggtactgctggtacctgcgccacacctacatgcagaacgagctcaaggtaccgggcaagctgaccgtgtgcggcgtgccggtggacctggccagcatcgacgtgccgacctatatctacggctcgcgcgaagaccatatcgtgccgtggaccgcggcctatgcctcgaccgcgctgctggcgaacaagctgcgcttcgtgctgggtgcgtcgggccatatcgccggtgtgatcaacccgccggccaagaacaagcgcagccactggactaacgatgcgctgccggagtcgccgcagcaatggctggccggcgccatcgagcatcacggcagctggtggccggactggaccgcatggctggccgggcaggccggcgcgaaacgcgccgcgcccgccaactatggcaatgcgcgctatcgcgcaatcgaacccgcgcctgggcgatacgtcaaagccaaggcatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K934001_sequence/1
type: Sequence
persistent identity: BBa K934001 sequence
display id: BBa_K934001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z