LOCUS BBa_K142003 1128 bp DNA linear 22-Dec-2024 DEFINITION lacI IS mutant (IPTG unresponsive) T276F ACCESSION BBa_K142003 VERSION BBa_K142003.1 FEATURES Location/Qualifiers CDS 1..1128 /label=lacI IS misc_feature 835..837 /label=T276F ORIGIN 1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgattttgaa 841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataa //