##gff-version 3
##sequence-region BBa_K142009 1 1283
BBa_K142009	.	terminator	1155	1234	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K142009	.	engineered_region	1155	1234	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142009	.	stem_loop	1166	1209	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142009	.	terminator	1243	1283	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K142009	.	engineered_region	1243	1283	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142009	.	polyA_site	1270	1283	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142009	.	stop_codon	1276	1276	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142009	.	stem_loop	1250	1269	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142009	.	CDS	19	1146	.	+	0	ID=BBa_K142001;Name=BBa_K142001
BBa_K142009	.	CDS	19	1146	.	+	0	ID=annotation1981799;Name=lacI IS;Parent=BBa_K142001
BBa_K142009	.	sequence_alteration	616	618	.	+	0	ID=annotation1981800;Name=R197F;Parent=BBa_K142001
BBa_K142009	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K142009	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K142009
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgtttctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata