LOCUS BBa_K142011 1283 bp DNA linear 15-Nov-2024 DEFINITION lacI IS mutant T276Fwith RBS and terminator ACCESSION BBa_K142011 VERSION BBa_K142011.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..1146 /label=lacI IS misc_feature 853..855 /label=T276F CDS 19..1146 /label=BBa_K142003 stem_loop 1166..1209 /label=stem_loop misc_feature 1155..1234 /label=BBa_B0010 terminator 1155..1234 /label=BBa_B0010 stem_loop 1250..1269 /label=T7 TE misc_feature 1276..1276 /label=stop polyA_site 1270..1283 /label=polya misc_feature 1243..1283 /label=BBa_B0012 terminator 1243..1283 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg attttgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 1201 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 1261 gggtgggcct ttctgcgttt ata //