##gff-version 3
##sequence-region BBa_K142025 1 2236
BBa_K142025	.	engineered_region	1354	2236	.	+	0	ID=BBa_I739001;Name=BBa_I739001
BBa_K142025	.	ribosome_entry_site	1397	1408	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739001
BBa_K142025	.	conserved_region	1401	1404	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142025	.	promoter	1354	1388	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739001
BBa_K142025	.	terminator	2108	2236	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739001
BBa_K142025	.	terminator	2108	2187	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142025	.	engineered_region	2108	2187	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142025	.	stem_loop	2119	2162	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142025	.	terminator	2196	2236	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142025	.	stop_codon	2229	2229	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142025	.	engineered_region	2196	2236	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142025	.	polyA_site	2223	2236	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142025	.	stem_loop	2203	2222	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142025	.	CDS	1415	2099	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I739001
BBa_K142025	.	tag	2035	2068	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K142025	.	engineered_tag	2075	2099	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K142025	.	CDS	1418	2034	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K142025	.	engineered_region	1	1345	.	+	0	ID=BBa_K142017;Name=BBa_K142017
BBa_K142025	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K142017
BBa_K142025	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K142025	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K142025	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K142025	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K142025	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K142025	.	terminator	1305	1345	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K142017
BBa_K142025	.	stop_codon	1338	1338	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142025	.	engineered_region	1305	1345	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142025	.	polyA_site	1332	1345	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142025	.	stem_loop	1312	1331	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142025	.	terminator	1217	1296	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K142017
BBa_K142025	.	engineered_region	1217	1296	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142025	.	stem_loop	1228	1271	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142025	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K142017
BBa_K142025	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142025	.	CDS	81	1208	.	+	0	ID=BBa_K142001;Name=BBa_K142001;Parent=BBa_K142017
BBa_K142025	.	CDS	81	1208	.	+	0	ID=annotation1981799;Name=lacI IS;Parent=BBa_K142001
BBa_K142025	.	sequence_alteration	678	680	.	+	0	ID=annotation1981800;Name=R197F;Parent=BBa_K142001
>BBa_K142025
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgtttctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccag
attagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaa
ctcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttag
ccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataa
cgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaa
cagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactca
gcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacc
tactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttctta
ttcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata