LOCUS       BBa_K142027             2236 bp    DNA     linear       20-May-2024
DEFINITION  IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) 
            and TetR expression cassette
ACCESSION   BBa_K142027
VERSION     BBa_K142027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1208
                     /label=lacI IS
     misc_feature    915..917
                     /label=T276F
     CDS             81..1208
                     /label=BBa_K142003
     stem_loop       1228..1271
                     /label=stem_loop
     misc_feature    1217..1296
                     /label=BBa_B0010
     terminator      1217..1296
                     /label=BBa_B0010
     stem_loop       1312..1331
                     /label=T7 TE
     misc_feature    1338..1338
                     /label=stop
     polyA_site      1332..1345
                     /label=polya
     misc_feature    1305..1345
                     /label=BBa_B0012
     terminator      1305..1345
                     /label=BBa_B0012
     CDS             1..1345
                     /label=BBa_K142019
     promoter        1354..1388
                     /label=BBa_J23105
     misc_feature    1401..1404
                     /label=conserved
     RBS             1397..1408
                     /label=BBa_B0034
     CDS             1418..2034
                     /label=tetR
     misc_feature    2035..2068
                     /label=SsrA
     misc_feature    2075..2099
                     /label=Help:Barcodes
     CDS             1415..2099
                     /label=BBa_C0040
     stem_loop       2119..2162
                     /label=stem_loop
     misc_feature    2108..2187
                     /label=BBa_B0010
     terminator      2108..2187
                     /label=BBa_B0010
     stem_loop       2203..2222
                     /label=T7 TE
     misc_feature    2229..2229
                     /label=stop
     polyA_site      2223..2236
                     /label=polya
     misc_feature    2196..2236
                     /label=BBa_B0012
     terminator      2196..2236
                     /label=BBa_B0012
     terminator      2108..2236
                     /label=BBa_B0015
     misc_feature    1354..2236
                     /label=BBa_I739001
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgattttgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1261 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1321 tcgggtgggc ctttctgcgt ttatatacta gagtttacgg ctagctcagt cctaggtact
     1381 atgctagcta ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt
     1441 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     1501 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     1561 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     1621 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
     1681 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
     1741 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
     1801 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
     1861 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
     1921 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
     1981 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc
     2041 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact
     2101 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2161 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2221 cctttctgcg tttata
//