LOCUS       BBa_K142047             2261 bp    DNA     linear       22-Dec-2024
DEFINITION  tet-controlled LacI generator with constitutive TetR expression 
            cassette
ACCESSION   BBa_K142047
VERSION     BBa_K142047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S0100
     misc_feature    1..1233
                     /label=BBa_I763025
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1..1370
                     /label=BBa_I763026
     misc_feature    1..1370
                     /label=BBa_K142046
     promoter        1379..1413
                     /label=BBa_J23105
     misc_feature    1426..1429
                     /label=conserved
     RBS             1422..1433
                     /label=BBa_B0034
     CDS             1443..2059
                     /label=tetR
     misc_feature    2060..2093
                     /label=SsrA
     misc_feature    2100..2124
                     /label=Help:Barcodes
     CDS             1440..2124
                     /label=BBa_C0040
     stem_loop       2144..2187
                     /label=stem_loop
     misc_feature    2133..2212
                     /label=BBa_B0010
     terminator      2133..2212
                     /label=BBa_B0010
     stem_loop       2228..2247
                     /label=T7 TE
     misc_feature    2254..2254
                     /label=stop
     polyA_site      2248..2261
                     /label=polya
     misc_feature    2221..2261
                     /label=BBa_B0012
     terminator      2221..2261
                     /label=BBa_B0012
     terminator      2133..2261
                     /label=BBa_B0015
     misc_feature    1379..2261
                     /label=BBa_I739001
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
     1381 tacggctagc tcagtcctag gtactatgct agctactaga gaaagaggag aaatactaga
     1441 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
     1501 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
     1561 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
     1621 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
     1681 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
     1741 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
     1801 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
     1861 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
     1921 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     1981 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     2041 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     2101 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
     2161 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2221 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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