##gff-version 3
##sequence-region BBa_K142047 1 2261
BBa_K142047	.	engineered_region	1	1370	.	+	0	ID=BBa_K142046;Name=BBa_K142046
BBa_K142047	.	engineered_region	1	1370	.	+	0	ID=BBa_I763026;Name=BBa_I763026;Parent=BBa_K142046
BBa_K142047	.	terminator	1242	1370	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I763026
BBa_K142047	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142047	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142047	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142047	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142047	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142047	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142047	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142047	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142047	.	engineered_region	1	1233	.	+	0	ID=BBa_I763025;Name=BBa_I763025;Parent=BBa_I763026
BBa_K142047	.	engineered_region	63	1233	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_K142047	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_K142047	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K142047	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K142047	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K142047	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K142047	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_K142047	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142047	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_K142047	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K142047	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K142047	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K142047	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K142047	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K142047	.	engineered_region	1379	2261	.	+	0	ID=BBa_I739001;Name=BBa_I739001
BBa_K142047	.	CDS	1440	2124	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I739001
BBa_K142047	.	tag	2060	2093	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K142047	.	CDS	1443	2059	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K142047	.	engineered_tag	2100	2124	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K142047	.	terminator	2133	2261	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739001
BBa_K142047	.	terminator	2221	2261	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142047	.	polyA_site	2248	2261	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142047	.	stem_loop	2228	2247	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142047	.	stop_codon	2254	2254	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142047	.	engineered_region	2221	2261	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142047	.	terminator	2133	2212	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142047	.	stem_loop	2144	2187	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142047	.	engineered_region	2133	2212	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142047	.	ribosome_entry_site	1422	1433	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739001
BBa_K142047	.	conserved_region	1426	1429	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142047	.	promoter	1379	1413	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739001
>BBa_K142047
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactagaga
aagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgg
aatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaat
aagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaa
gctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtaca
tttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttca
ctagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaag
tcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatca
ccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagt
gggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata