LOCUS       BBa_K142048             2181 bp    DNA     linear       22-Nov-2024
DEFINITION  promotorless lacI expression cassette followed by constitutively 
            active TetR generator
ACCESSION   BBa_K142048
VERSION     BBa_K142048.1  
FEATURES             Location/Qualifiers
     misc_feature    1090..1128
                     /label=LVA
     CDS             1..1128
                     /label=lacI-LVA
     misc_feature    1..1128
                     /label=BBa_C0012
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             1..1128
                     /label=BBa_C0012
     stem_loop       1173..1216
                     /label=stem_loop
     misc_feature    1162..1241
                     /label=BBa_B0010
     terminator      1162..1241
                     /label=BBa_B0010
     stem_loop       1257..1276
                     /label=T7 TE
     misc_feature    1283..1283
                     /label=stop
     polyA_site      1277..1290
                     /label=polya
     misc_feature    1250..1290
                     /label=BBa_B0012
     terminator      1250..1290
                     /label=BBa_B0012
     promoter        1299..1333
                     /label=BBa_J23105
     misc_feature    1346..1349
                     /label=conserved
     RBS             1342..1353
                     /label=BBa_B0034
     CDS             1363..1979
                     /label=tetR
     misc_feature    1980..2013
                     /label=SsrA
     misc_feature    2020..2044
                     /label=Help:Barcodes
     CDS             1360..2044
                     /label=BBa_C0040
     stem_loop       2064..2107
                     /label=stem_loop
     misc_feature    2053..2132
                     /label=BBa_B0010
     terminator      2053..2132
                     /label=BBa_B0010
     stem_loop       2148..2167
                     /label=T7 TE
     misc_feature    2174..2174
                     /label=stop
     polyA_site      2168..2181
                     /label=polya
     misc_feature    2141..2181
                     /label=BBa_B0012
     terminator      2141..2181
                     /label=BBa_B0012
     terminator      2053..2181
                     /label=BBa_B0015
     misc_feature    1299..2181
                     /label=BBa_I739001
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1261 caccttcggg tgggcctttc tgcgtttata tactagagtt tacggctagc tcagtcctag
     1321 gtactatgct agctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
     1381 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     1441 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     1501 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     1561 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     1621 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     1681 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     1741 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     1801 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     1861 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     1921 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
     1981 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
     2041 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2101 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2161 gtgggccttt ctgcgtttat a
//