##gff-version 3
##sequence-region BBa_K142048 1 2181
BBa_K142048	.	terminator	1250	1290	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K142048	.	engineered_region	1250	1290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142048	.	stop_codon	1283	1283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142048	.	stem_loop	1257	1276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142048	.	polyA_site	1277	1290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142048	.	terminator	1162	1241	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K142048	.	engineered_region	1162	1241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142048	.	stem_loop	1173	1216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142048	.	engineered_region	1299	2181	.	+	0	ID=BBa_I739001;Name=BBa_I739001
BBa_K142048	.	ribosome_entry_site	1342	1353	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739001
BBa_K142048	.	conserved_region	1346	1349	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142048	.	CDS	1360	2044	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I739001
BBa_K142048	.	engineered_tag	2020	2044	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K142048	.	tag	1980	2013	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K142048	.	CDS	1363	1979	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K142048	.	terminator	2053	2181	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739001
BBa_K142048	.	terminator	2053	2132	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142048	.	engineered_region	2053	2132	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142048	.	stem_loop	2064	2107	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142048	.	terminator	2141	2181	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142048	.	engineered_region	2141	2181	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142048	.	stop_codon	2174	2174	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142048	.	stem_loop	2148	2167	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142048	.	polyA_site	2168	2181	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142048	.	promoter	1299	1333	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739001
BBa_K142048	.	CDS	1	1128	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K142048	.	engineered_tag	1129	1153	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K142048	.	engineered_region	1	1128	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K142048	.	CDS	1	1128	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K142048	.	tag	1090	1128	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_K142048
atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtgg
tgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaa
ccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg
tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcg
gcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgga
tgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacaccc
atcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaa
tcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaa
tcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaat
gagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccg
ggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaac
caccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtg
aagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcccc
gcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaa
ctacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactagagaa
agaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgga
atcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaata
agcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaag
ctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcac
tagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagt
cgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcac
caaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtg
ggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata