LOCUS       BBa_K142073             2442 bp    DNA     linear       25-Nov-2024
DEFINITION  lac-controlled GFP generator with constitutive lacI expression 
            cassette
ACCESSION   BBa_K142073
VERSION     BBa_K142073.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
     misc_feature    1..1083
                     /label=BBa_J04430
     promoter        1092..1126
                     /label=BBa_J23105
     misc_feature    1139..1142
                     /label=conserved
     RBS             1135..1146
                     /label=BBa_B0034
     misc_feature    2242..2280
                     /label=LVA
     CDS             1153..2280
                     /label=lacI-LVA
     misc_feature    1153..2280
                     /label=BBa_C0012
     misc_feature    2281..2305
                     /label=Help:Barcodes
     CDS             1153..2280
                     /label=BBa_C0012
     stem_loop       2325..2368
                     /label=stem_loop
     misc_feature    2314..2393
                     /label=BBa_B0010
     terminator      2314..2393
                     /label=BBa_B0010
     stem_loop       2409..2428
                     /label=T7 TE
     misc_feature    2435..2435
                     /label=stop
     polyA_site      2429..2442
                     /label=polya
     misc_feature    2402..2442
                     /label=BBa_B0012
     terminator      2402..2442
                     /label=BBa_B0012
     terminator      2314..2442
                     /label=BBa_B0015
     misc_feature    1092..2442
                     /label=BBa_I739002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 atatactaga gtttacggct agctcagtcc taggtactat gctagctact agagaaagag
     1141 gagaaatact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
     1201 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
     1261 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
     1321 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
     1381 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
     1441 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
     1501 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
     1561 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
     1621 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
     1681 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
     1741 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
     1801 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
     1861 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
     1921 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
     1981 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     2041 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     2101 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     2161 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     2221 tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa
     2281 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc
     2341 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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