##gff-version 3
##sequence-region BBa_K142073 1 2442
BBa_K142073	.	engineered_region	1092	2442	.	+	0	ID=BBa_I739002;Name=BBa_I739002
BBa_K142073	.	ribosome_entry_site	1135	1146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739002
BBa_K142073	.	conserved_region	1139	1142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142073	.	terminator	2314	2442	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739002
BBa_K142073	.	terminator	2402	2442	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142073	.	engineered_region	2402	2442	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142073	.	stop_codon	2435	2435	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142073	.	polyA_site	2429	2442	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142073	.	stem_loop	2409	2428	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142073	.	terminator	2314	2393	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142073	.	engineered_region	2314	2393	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142073	.	stem_loop	2325	2368	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142073	.	promoter	1092	1126	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739002
BBa_K142073	.	CDS	1153	2280	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I739002
BBa_K142073	.	engineered_tag	2281	2305	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K142073	.	engineered_region	1153	2280	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K142073	.	CDS	1153	2280	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K142073	.	tag	2242	2280	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K142073	.	engineered_region	1	1083	.	+	0	ID=BBa_J04430;Name=BBa_J04430
BBa_K142073	.	CDS	227	946	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J04430
BBa_K142073	.	CDS	227	946	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K142073	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04430
BBa_K142073	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K142073	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K142073	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K142073	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K142073	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K142073	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K142073	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K142073	.	terminator	1043	1083	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04430
BBa_K142073	.	engineered_region	1043	1083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142073	.	stop_codon	1076	1076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142073	.	polyA_site	1070	1083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142073	.	stem_loop	1050	1069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142073	.	terminator	955	1034	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04430
BBa_K142073	.	engineered_region	955	1034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142073	.	stem_loop	966	1009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142073	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04430
BBa_K142073	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K142073
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctca
gtcctaggtactatgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgat
gtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgc
gggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtc
gttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgcc
gatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatc
ttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctg
cactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggt
acgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtct
cggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcga
ctggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgcc
aacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgg
gatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggca
aaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtg
aaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcac
gacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata