LOCUS       BBa_K142074             2481 bp    DNA     linear       17-May-2024
DEFINITION  lac-controlled GFP-LVA generator with constitutive lacI expression 
            cassette
ACCESSION   BBa_K142074
VERSION     BBa_K142074.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..946
                     /label=BBa_E0040
     misc_feature    941..979
                     /label=LVA
     misc_feature    980..985
                     /label=Double Stop
     misc_feature    227..229
                     /label=Start
     misc_feature    227..985
                     /label=BBa_J04031
     stem_loop       1005..1048
                     /label=stem_loop
     misc_feature    994..1073
                     /label=BBa_B0010
     terminator      994..1073
                     /label=BBa_B0010
     stem_loop       1089..1108
                     /label=T7 TE
     misc_feature    1115..1115
                     /label=stop
     polyA_site      1109..1122
                     /label=polya
     misc_feature    1082..1122
                     /label=BBa_B0012
     terminator      1082..1122
                     /label=BBa_B0012
     misc_feature    1..1122
                     /label=BBa_I763004
     promoter        1131..1165
                     /label=BBa_J23105
     misc_feature    1178..1181
                     /label=conserved
     RBS             1174..1185
                     /label=BBa_B0034
     misc_feature    2281..2319
                     /label=LVA
     CDS             1192..2319
                     /label=lacI-LVA
     misc_feature    1192..2319
                     /label=BBa_C0012
     misc_feature    2320..2344
                     /label=Help:Barcodes
     CDS             1192..2319
                     /label=BBa_C0012
     stem_loop       2364..2407
                     /label=stem_loop
     misc_feature    2353..2432
                     /label=BBa_B0010
     terminator      2353..2432
                     /label=BBa_B0010
     stem_loop       2448..2467
                     /label=T7 TE
     misc_feature    2474..2474
                     /label=stop
     polyA_site      2468..2481
                     /label=polya
     misc_feature    2441..2481
                     /label=BBa_B0012
     terminator      2441..2481
                     /label=BBa_B0012
     terminator      2353..2481
                     /label=BBa_B0015
     misc_feature    1131..2481
                     /label=BBa_I739002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa aggcctgctg caaacgacga
      961 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1021 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1081 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tttacggcta
     1141 gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta gatggtgaat
     1201 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     1261 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     1321 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     1381 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     1441 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     1501 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     1561 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     1621 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     1681 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     1741 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     1801 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     1861 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     1921 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     1981 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     2041 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     2101 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     2161 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     2221 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2281 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
     2341 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2401 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2461 gtgggccttt ctgcgtttat a
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