##gff-version 3
##sequence-region BBa_K142074 1 2481
BBa_K142074	.	engineered_region	1131	2481	.	+	0	ID=BBa_I739002;Name=BBa_I739002
BBa_K142074	.	CDS	1192	2319	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I739002
BBa_K142074	.	engineered_tag	2320	2344	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K142074	.	CDS	1192	2319	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K142074	.	engineered_region	1192	2319	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K142074	.	tag	2281	2319	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K142074	.	promoter	1131	1165	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739002
BBa_K142074	.	ribosome_entry_site	1174	1185	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739002
BBa_K142074	.	conserved_region	1178	1181	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142074	.	terminator	2353	2481	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739002
BBa_K142074	.	terminator	2441	2481	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K142074	.	stem_loop	2448	2467	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142074	.	polyA_site	2468	2481	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142074	.	stop_codon	2474	2474	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142074	.	engineered_region	2441	2481	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142074	.	terminator	2353	2432	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K142074	.	stem_loop	2364	2407	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142074	.	engineered_region	2353	2432	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142074	.	engineered_region	1	1122	.	+	0	ID=BBa_I763004;Name=BBa_I763004
BBa_K142074	.	terminator	994	1073	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763004
BBa_K142074	.	stem_loop	1005	1048	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142074	.	engineered_region	994	1073	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142074	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I763004
BBa_K142074	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K142074	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K142074	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K142074	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K142074	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K142074	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K142074	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K142074	.	engineered_region	227	985	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763004
BBa_K142074	.	stop_codon	980	985	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K142074	.	tag	941	979	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K142074	.	engineered_region	227	946	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K142074	.	start_codon	227	229	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K142074	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763004
BBa_K142074	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K142074	.	terminator	1082	1122	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763004
BBa_K142074	.	stem_loop	1089	1108	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142074	.	polyA_site	1109	1122	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142074	.	stop_codon	1115	1115	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142074	.	engineered_region	1082	1122	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K142074
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgc
tgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaa
tactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttccc
gcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacat
tcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcac
gcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaac
gaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatcc
gctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccag
acacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacc
agcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcac
tcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatg
ctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg
agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgcc
gttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag
gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcct
ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata