LOCUS       BBa_K142075             2300 bp    DNA     linear       26-Dec-2024
DEFINITION  lac-controlled CFP generator with constitutive lacI expression 
            cassette
ACCESSION   BBa_K142075
VERSION     BBa_K142075.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=BBa_E0020
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    64..941
                     /label=BBa_E0420
     misc_feature    1..941
                     /label=BBa_I13601
     promoter        950..984
                     /label=BBa_J23105
     misc_feature    997..1000
                     /label=conserved
     RBS             993..1004
                     /label=BBa_B0034
     misc_feature    2100..2138
                     /label=LVA
     CDS             1011..2138
                     /label=lacI-LVA
     misc_feature    1011..2138
                     /label=BBa_C0012
     misc_feature    2139..2163
                     /label=Help:Barcodes
     CDS             1011..2138
                     /label=BBa_C0012
     stem_loop       2183..2226
                     /label=stem_loop
     misc_feature    2172..2251
                     /label=BBa_B0010
     terminator      2172..2251
                     /label=BBa_B0010
     stem_loop       2267..2286
                     /label=T7 TE
     misc_feature    2293..2293
                     /label=stop
     polyA_site      2287..2300
                     /label=polya
     misc_feature    2260..2300
                     /label=BBa_B0012
     terminator      2260..2300
                     /label=BBa_B0012
     terminator      2172..2300
                     /label=BBa_B0015
     misc_feature    950..2300
                     /label=BBa_I739002
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
      541 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ttacggctag
      961 ctcagtccta ggtactatgc tagctactag agaaagagga gaaatactag atggtgaatg
     1021 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     1081 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     1141 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     1201 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     1261 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     1321 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     1381 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     1441 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     1501 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     1561 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     1621 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     1681 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     1741 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     1801 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     1861 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     1921 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     1981 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     2041 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg
     2101 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
     2161 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2221 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2281 tgggcctttc tgcgtttata
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