LOCUS       BBa_K142076             2428 bp    DNA     linear       22-Dec-2024
DEFINITION  lac-controlled RFP generator with constitutive lacI expression 
            cassette
ACCESSION   BBa_K142076
VERSION     BBa_K142076.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     promoter        1078..1112
                     /label=BBa_J23105
     misc_feature    1125..1128
                     /label=conserved
     RBS             1121..1132
                     /label=BBa_B0034
     misc_feature    2228..2266
                     /label=LVA
     CDS             1139..2266
                     /label=lacI-LVA
     misc_feature    1139..2266
                     /label=BBa_C0012
     misc_feature    2267..2291
                     /label=Help:Barcodes
     CDS             1139..2266
                     /label=BBa_C0012
     stem_loop       2311..2354
                     /label=stem_loop
     misc_feature    2300..2379
                     /label=BBa_B0010
     terminator      2300..2379
                     /label=BBa_B0010
     stem_loop       2395..2414
                     /label=T7 TE
     misc_feature    2421..2421
                     /label=stop
     polyA_site      2415..2428
                     /label=polya
     misc_feature    2388..2428
                     /label=BBa_B0012
     terminator      2388..2428
                     /label=BBa_B0012
     terminator      2300..2428
                     /label=BBa_B0015
     misc_feature    1078..2428
                     /label=BBa_I739002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttt
     1081 acggctagct cagtcctagg tactatgcta gctactagag aaagaggaga aatactagat
     1141 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     1201 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     1261 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     1321 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     1381 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     1441 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     1501 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     1561 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     1621 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     1681 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     1741 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     1801 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     1861 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     1921 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     1981 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     2041 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     2101 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     2161 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2221 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta
     2281 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2341 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2401 ccttcgggtg ggcctttctg cgtttata
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