LOCUS BBa_K1421001 1301 bp DNA linear 05-Nov-2024 DEFINITION plux R+RBS+LacIM1+2TE ACCESSION BBa_K1421001 VERSION BBa_K1421001.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..1164 /label=BBa_K082005 CDS 82..1164 /label=BBa_K082005 stem_loop 1184..1227 /label=stem_loop misc_feature 1173..1252 /label=BBa_B0010 terminator 1173..1252 /label=BBa_B0010 stem_loop 1268..1287 /label=T7 TE misc_feature 1294..1294 /label=stop polyA_site 1288..1301 /label=polya misc_feature 1261..1301 /label=BBa_B0012 terminator 1261..1301 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgaagccg gtgaccctgt atgacgtggc ggaatacgcg 121 ggcgtgagct accaaacggt gagccgtgtt gttaatcaag cgtctcatgt gagcgccaag 181 acccgcgaga aggttgaggc cgccatggcc gaattaaact atatcccgaa tcgtgttgcg 241 cagcagttag ccggtaagca aagcctgtta atcggtgtgg cgacgagcag cttagcgtca 301 catgccccaa gccagatcgt ggccgccatc aagagccgtg cggaccagtt aggcgcgtct 361 gttgttgtta gaatggtgga gcgctctggt gtggaggcgt gcaaggccgc cgttcataac 421 ctgctggccc agcgtgtgag cggcttaatt atcaattacc cattagacga tcaagacgcg 481 atcgcggttg aggcggcgtg taccaacgtg ccagccctgt tcctggacgt gagcgatcaa 541 accccgatta atagcatcat ctttagccac gagaatggca cccgcttagg tgttgaacac 601 ttagtggccc tgggccatca acagattgcc ctactggccg gtccgctgag cagcgtgagc 661 gcccgcttac gcttagcggg ttggcacaag tacctgaccc gtaaccagat ccaaccaatt 721 gccgagcgcg agggtgattg gagcgcgatg agcggcttcc agcagacgat gcagatgtta 781 aacgaaggta ttgtgccgac cgccatgtta gtggcgaatg accaaatggc cttaggtgcg 841 atgcgtgcga tcacggaaag cggcttacgt gtgggcgccg acattagcgt ggttggctat 901 gatgacacgg aggattctag ctgctacatt ccaccaagca cgacgattaa gcaagacttc 961 cgtttattag gccagacgtc tgttgatcgt ctgttacagc tgagccaagg tcaagccgtt 1021 aaaggtaacc aattactgcc ggtgagctta gttaagcgca agacgacgtt agccccgaac 1081 acccagacgg cgagcccgcg tgccctggcg gacagcctga tgcaattagc gcgccaagtg 1141 agccgcttag agtctggcca atgatactag agccaggcat caaataaaac gaaaggctca 1201 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1261 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //