LOCUS       BBa_K1421008            1298 bp    DNA     linear       06-Nov-2024
DEFINITION  pCl+RBS+LacI+2TE
ACCESSION   BBa_K1421008
VERSION     BBa_K1421008.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..1161
                     /label=BBa_I732100
     stem_loop       1181..1224
                     /label=stem_loop
     misc_feature    1170..1249
                     /label=BBa_B0010
     terminator      1170..1249
                     /label=BBa_B0010
     stem_loop       1265..1284
                     /label=T7 TE
     misc_feature    1291..1291
                     /label=stop
     polyA_site      1285..1298
                     /label=polya
     misc_feature    1258..1298
                     /label=BBa_B0012
     terminator      1258..1298
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      121 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      181 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      241 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      301 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
      361 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
      421 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
      481 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
      541 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
      601 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
      661 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
      721 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
      781 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
      841 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
      901 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
      961 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     1021 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     1081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1141 ctggaaagcg ggcagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1201 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1261 cactggctca ccttcgggtg ggcctttctg cgtttata
//