LOCUS       BBa_K1421009            1718 bp    DNA     linear       22-Nov-2024
DEFINITION  plac+RBS+RFP+luxI+2TE
ACCESSION   BBa_K1421009
VERSION     BBa_K1421009.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..932
                     /label=BBa_E1010
     misc_feature    1518..1549
                     /label=LVA
     CDS             939..1517
                     /label=luxI
     misc_feature    939..1556
                     /label=BBa_C0061
     misc_feature    1557..1581
                     /label=Help:Barcodes
     CDS             939..1556
                     /label=BBa_C0061
     stem_loop       1601..1644
                     /label=stem_loop
     misc_feature    1590..1669
                     /label=BBa_B0010
     terminator      1590..1669
                     /label=BBa_B0010
     stem_loop       1685..1704
                     /label=T7 TE
     misc_feature    1711..1711
                     /label=stop
     polyA_site      1705..1718
                     /label=polya
     misc_feature    1678..1718
                     /label=BBa_B0012
     terminator      1678..1718
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagat gactataatg ataaaaaaat
      961 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag
     1021 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt
     1081 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat
     1141 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc
     1201 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag
     1261 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag
     1321 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag
     1381 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa
     1441 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta
     1501 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct
     1561 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1621 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1681 cactggctca ccttcgggtg ggcctttctg cgtttata
//