LOCUS       BBa_K142202              906 bp    DNA     linear       25-Nov-2024
DEFINITION  SceI with RBS (BBa_B0030) and double terminator (BBa_B0015)
ACCESSION   BBa_K142202
VERSION     BBa_K142202.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..769
                     /label=SceI
     CDS             22..769
                     /label=BBa_K142200
     stem_loop       789..832
                     /label=stem_loop
     misc_feature    778..857
                     /label=BBa_B0010
     terminator      778..857
                     /label=BBa_B0010
     stem_loop       873..892
                     /label=T7 TE
     misc_feature    899..899
                     /label=stop
     polyA_site      893..906
                     /label=polya
     misc_feature    866..906
                     /label=BBa_B0012
     terminator      866..906
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgcatcaa aaaaaccagg taatgaacct gggtccgaac
       61 tctaaactgc tgaaagaata caaatcccag ctgatcgaac tgaacatcga acagttcgaa
      121 gcaggtatcg gtctgatcct gggtgatgct tacatccgtt ctcgtgatga aggtaaaacc
      181 tactgtatgc agttcgagtg gaaaaacaaa gcatacatgg accacgtatg tctgctgtac
      241 gatcagtggg tactgtcccc gccgcacaaa aaagaacgtg ttaaccacct gggtaacctg
      301 gtaatcacct ggggcgccca gactttcaaa caccaagcct tcaacaaact ggctaacctg
      361 ttcatcgtta acaacaaaaa aaccatcccg aacaacctgg ttgaaaacta cctgaccccg
      421 atgtctctgg catactggtt catggatgat ggtggtaaat gggattacaa caaaaactct
      481 accaacaaat cgatcgtact gaacacccag tctttcactt tcgaagaagt agaatacctg
      541 gttaagggtc tgcgtaacaa attccaactg aactgttacg taaaaatcaa caaaaacaaa
      601 ccgatcatct acatcgattc tatgtcttac ctgatcttct acaacctgat caaaccgtac
      661 ctgatcccgc agatgatgta caaactgccg aacactatct cctccgaaac tttccttaag
      721 aggcccgctg caaacgacga aaactacgct ttagtagctt aagtaataat actagagcca
      781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      901 tttata
//