LOCUS       BBa_K142203             1625 bp    DNA     linear       22-Dec-2024
DEFINITION  T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)
ACCESSION   BBa_K142203
VERSION     BBa_K142203.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1488
                     /label=T4 ligase
     CDS             22..1488
                     /label=BBa_K142201
     stem_loop       1508..1551
                     /label=stem_loop
     misc_feature    1497..1576
                     /label=BBa_B0010
     terminator      1497..1576
                     /label=BBa_B0010
     stem_loop       1592..1611
                     /label=T7 TE
     misc_feature    1618..1618
                     /label=stop
     polyA_site      1612..1625
                     /label=polya
     misc_feature    1585..1625
                     /label=BBa_B0012
     terminator      1585..1625
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgattctt aaaattctga acgaaatagc atctattggt
       61 tcaactaaac agaagcaagc aattcttgaa aagaataaag ataatgaatt gcttaaacga
      121 gtatatcgtc tgacttattc tcgtgggtta cagtattata tcaagaaatg gcctaaacct
      181 ggtattgcta cccagagttt tggaatgttg actcttaccg atatgcttga cttcattgag
      241 ttcacattag ctactcggaa attgactgga aatgcagcaa ttgaggaatt aactggatat
      301 atcaccgatg gtaaaaaaga tgatgttgaa gttttgcgtc gagtgatgat gcgagacctt
      361 gaatgtggtg cttcagtatc tattgcaaac aaagtttggc caggtttaat tcctgaacaa
      421 cctcaaatgc tcgcaagttc ttatgatgaa aaaggcatta ataagaatat caaatttcca
      481 gcctttgctc agttaaaagc tgatggagct cggtgttttg ctgaagttag aggtgatgaa
      541 ttagatgatg ttcgtctttt atcacgagct ggtaatgaat atctaggatt agaccttctt
      601 aaggaagagt taattaaaat gaccgctgaa gcccgccaga ttcatccaga aggtgtgttg
      661 attgatggcg aattggtata ccatgagcaa gttaaaaagg agccagaagg cctagatttt
      721 ctttttgatg cttatcctga aaacagtaaa gctaaagagt tcgccgaagt agctgaatca
      781 cgtactgctt ctaatggaat cgccaataaa tctttaaagg gaaccatttc tgaaaaagaa
      841 gcacaatgca tgaagtttca ggtctgggat tatgtcccgt tggtagaaat atacagtctt
      901 cctgcatttc gtttgaaata tgatgtacgt ttttctaaac tagaacaaat gacatctgga
      961 tatgataaag taattttaat tgaaaaccag gtagtaaata acctagatga agctaaggta
     1021 atttataaaa agtatattga ccaaggtctt gaaggtatta ttctcaaaaa tatcgatgga
     1081 ttatgggaaa atgctcgttc aaaaaatctt tataaattta aagaagtaat tgatgttgat
     1141 ttaaaaattg taggaattta tcctcaccgt aaagacccta ctaaagcggg tggatttatt
     1201 cttgagtcag agtgtggaaa aattaaggta aatgctggtt caggcttaaa agataaagcc
     1261 ggtgtaaaat cgcatgaact tgaccgtact cgcattatgg aaaaccaaaa ttattatatt
     1321 ggaaaaattc tcgagtgcga atgcaacggt tggttaaaat ctgatggccg cactgattac
     1381 gttaaattat ttcttccgat tgcgattcgt ttacgtgaag ataaaactaa agctaataca
     1441 ttcgaagatg tatttggtga ttttcatgag gtaactggtc tatgataata ctagagccag
     1501 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1561 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1621 ttata
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