LOCUS       BBa_K142204             1833 bp    DNA     linear       25-Nov-2024
DEFINITION  lac Promoter + RBS + T4 ligase + double terminator
ACCESSION   BBa_K142204
VERSION     BBa_K142204.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1696
                     /label=T4 ligase
     CDS             230..1696
                     /label=BBa_K142201
     stem_loop       1716..1759
                     /label=stem_loop
     misc_feature    1705..1784
                     /label=BBa_B0010
     terminator      1705..1784
                     /label=BBa_B0010
     stem_loop       1800..1819
                     /label=T7 TE
     misc_feature    1826..1826
                     /label=stop
     polyA_site      1820..1833
                     /label=polya
     misc_feature    1793..1833
                     /label=BBa_B0012
     terminator      1793..1833
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgattcttaa
      241 aattctgaac gaaatagcat ctattggttc aactaaacag aagcaagcaa ttcttgaaaa
      301 gaataaagat aatgaattgc ttaaacgagt atatcgtctg acttattctc gtgggttaca
      361 gtattatatc aagaaatggc ctaaacctgg tattgctacc cagagttttg gaatgttgac
      421 tcttaccgat atgcttgact tcattgagtt cacattagct actcggaaat tgactggaaa
      481 tgcagcaatt gaggaattaa ctggatatat caccgatggt aaaaaagatg atgttgaagt
      541 tttgcgtcga gtgatgatgc gagaccttga atgtggtgct tcagtatcta ttgcaaacaa
      601 agtttggcca ggtttaattc ctgaacaacc tcaaatgctc gcaagttctt atgatgaaaa
      661 aggcattaat aagaatatca aatttccagc ctttgctcag ttaaaagctg atggagctcg
      721 gtgttttgct gaagttagag gtgatgaatt agatgatgtt cgtcttttat cacgagctgg
      781 taatgaatat ctaggattag accttcttaa ggaagagtta attaaaatga ccgctgaagc
      841 ccgccagatt catccagaag gtgtgttgat tgatggcgaa ttggtatacc atgagcaagt
      901 taaaaaggag ccagaaggcc tagattttct ttttgatgct tatcctgaaa acagtaaagc
      961 taaagagttc gccgaagtag ctgaatcacg tactgcttct aatggaatcg ccaataaatc
     1021 tttaaaggga accatttctg aaaaagaagc acaatgcatg aagtttcagg tctgggatta
     1081 tgtcccgttg gtagaaatat acagtcttcc tgcatttcgt ttgaaatatg atgtacgttt
     1141 ttctaaacta gaacaaatga catctggata tgataaagta attttaattg aaaaccaggt
     1201 agtaaataac ctagatgaag ctaaggtaat ttataaaaag tatattgacc aaggtcttga
     1261 aggtattatt ctcaaaaata tcgatggatt atgggaaaat gctcgttcaa aaaatcttta
     1321 taaatttaaa gaagtaattg atgttgattt aaaaattgta ggaatttatc ctcaccgtaa
     1381 agaccctact aaagcgggtg gatttattct tgagtcagag tgtggaaaaa ttaaggtaaa
     1441 tgctggttca ggcttaaaag ataaagccgg tgtaaaatcg catgaacttg accgtactcg
     1501 cattatggaa aaccaaaatt attatattgg aaaaattctc gagtgcgaat gcaacggttg
     1561 gttaaaatct gatggccgca ctgattacgt taaattattt cttccgattg cgattcgttt
     1621 acgtgaagat aaaactaaag ctaatacatt cgaagatgta tttggtgatt ttcatgaggt
     1681 aactggtcta tgataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1741 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1801 gctcaccttc gggtgggcct ttctgcgttt ata
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