##gff-version 3
##sequence-region BBa_K142204 1 1833
BBa_K142204	.	terminator	1705	1784	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K142204	.	stem_loop	1716	1759	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K142204	.	engineered_region	1705	1784	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K142204	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K142204	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K142204	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K142204	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K142204	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K142204	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K142204	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K142204	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K142204	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K142204	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K142204	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K142204	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K142204	.	terminator	1793	1833	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K142204	.	polyA_site	1820	1833	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K142204	.	stem_loop	1800	1819	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K142204	.	stop_codon	1826	1826	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K142204	.	engineered_region	1793	1833	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K142204	.	CDS	230	1696	.	+	0	ID=BBa_K142201;Name=BBa_K142201
BBa_K142204	.	CDS	230	1696	.	+	0	ID=annotation1993901;Name=T4 ligase;Parent=BBa_K142201
>BBa_K142204
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgattct
taaaattctgaacgaaatagcatctattggttcaactaaacagaagcaagcaattcttgaaaagaataaagataatgaa
ttgcttaaacgagtatatcgtctgacttattctcgtgggttacagtattatatcaagaaatggcctaaacctggtattg
ctacccagagttttggaatgttgactcttaccgatatgcttgacttcattgagttcacattagctactcggaaattgac
tggaaatgcagcaattgaggaattaactggatatatcaccgatggtaaaaaagatgatgttgaagttttgcgtcgagtg
atgatgcgagaccttgaatgtggtgcttcagtatctattgcaaacaaagtttggccaggtttaattcctgaacaacctc
aaatgctcgcaagttcttatgatgaaaaaggcattaataagaatatcaaatttccagcctttgctcagttaaaagctga
tggagctcggtgttttgctgaagttagaggtgatgaattagatgatgttcgtcttttatcacgagctggtaatgaatat
ctaggattagaccttcttaaggaagagttaattaaaatgaccgctgaagcccgccagattcatccagaaggtgtgttga
ttgatggcgaattggtataccatgagcaagttaaaaaggagccagaaggcctagattttctttttgatgcttatcctga
aaacagtaaagctaaagagttcgccgaagtagctgaatcacgtactgcttctaatggaatcgccaataaatctttaaag
ggaaccatttctgaaaaagaagcacaatgcatgaagtttcaggtctgggattatgtcccgttggtagaaatatacagtc
ttcctgcatttcgtttgaaatatgatgtacgtttttctaaactagaacaaatgacatctggatatgataaagtaatttt
aattgaaaaccaggtagtaaataacctagatgaagctaaggtaatttataaaaagtatattgaccaaggtcttgaaggt
attattctcaaaaatatcgatggattatgggaaaatgctcgttcaaaaaatctttataaatttaaagaagtaattgatg
ttgatttaaaaattgtaggaatttatcctcaccgtaaagaccctactaaagcgggtggatttattcttgagtcagagtg
tggaaaaattaaggtaaatgctggttcaggcttaaaagataaagccggtgtaaaatcgcatgaacttgaccgtactcgc
attatggaaaaccaaaattattatattggaaaaattctcgagtgcgaatgcaacggttggttaaaatctgatggccgca
ctgattacgttaaattatttcttccgattgcgattcgtttacgtgaagataaaactaaagctaatacattcgaagatgt
atttggtgattttcatgaggtaactggtctatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata