LOCUS       BBa_K142207             1668 bp    DNA     linear       23-Dec-2024
DEFINITION  medium constitutive T4 ligase generator
ACCESSION   BBa_K142207
VERSION     BBa_K142207.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..1531
                     /label=T4 ligase
     CDS             65..1531
                     /label=BBa_K142201
     stem_loop       1551..1594
                     /label=stem_loop
     misc_feature    1540..1619
                     /label=BBa_B0010
     terminator      1540..1619
                     /label=BBa_B0010
     stem_loop       1635..1654
                     /label=T7 TE
     misc_feature    1661..1661
                     /label=stop
     polyA_site      1655..1668
                     /label=polya
     misc_feature    1628..1668
                     /label=BBa_B0012
     terminator      1628..1668
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgatt cttaaaattc tgaacgaaat agcatctatt ggttcaacta aacagaagca
      121 agcaattctt gaaaagaata aagataatga attgcttaaa cgagtatatc gtctgactta
      181 ttctcgtggg ttacagtatt atatcaagaa atggcctaaa cctggtattg ctacccagag
      241 ttttggaatg ttgactctta ccgatatgct tgacttcatt gagttcacat tagctactcg
      301 gaaattgact ggaaatgcag caattgagga attaactgga tatatcaccg atggtaaaaa
      361 agatgatgtt gaagttttgc gtcgagtgat gatgcgagac cttgaatgtg gtgcttcagt
      421 atctattgca aacaaagttt ggccaggttt aattcctgaa caacctcaaa tgctcgcaag
      481 ttcttatgat gaaaaaggca ttaataagaa tatcaaattt ccagcctttg ctcagttaaa
      541 agctgatgga gctcggtgtt ttgctgaagt tagaggtgat gaattagatg atgttcgtct
      601 tttatcacga gctggtaatg aatatctagg attagacctt cttaaggaag agttaattaa
      661 aatgaccgct gaagcccgcc agattcatcc agaaggtgtg ttgattgatg gcgaattggt
      721 ataccatgag caagttaaaa aggagccaga aggcctagat tttctttttg atgcttatcc
      781 tgaaaacagt aaagctaaag agttcgccga agtagctgaa tcacgtactg cttctaatgg
      841 aatcgccaat aaatctttaa agggaaccat ttctgaaaaa gaagcacaat gcatgaagtt
      901 tcaggtctgg gattatgtcc cgttggtaga aatatacagt cttcctgcat ttcgtttgaa
      961 atatgatgta cgtttttcta aactagaaca aatgacatct ggatatgata aagtaatttt
     1021 aattgaaaac caggtagtaa ataacctaga tgaagctaag gtaatttata aaaagtatat
     1081 tgaccaaggt cttgaaggta ttattctcaa aaatatcgat ggattatggg aaaatgctcg
     1141 ttcaaaaaat ctttataaat ttaaagaagt aattgatgtt gatttaaaaa ttgtaggaat
     1201 ttatcctcac cgtaaagacc ctactaaagc gggtggattt attcttgagt cagagtgtgg
     1261 aaaaattaag gtaaatgctg gttcaggctt aaaagataaa gccggtgtaa aatcgcatga
     1321 acttgaccgt actcgcatta tggaaaacca aaattattat attggaaaaa ttctcgagtg
     1381 cgaatgcaac ggttggttaa aatctgatgg ccgcactgat tacgttaaat tatttcttcc
     1441 gattgcgatt cgtttacgtg aagataaaac taaagctaat acattcgaag atgtatttgg
     1501 tgattttcat gaggtaactg gtctatgata atactagagc caggcatcaa ataaaacgaa
     1561 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1621 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//