BBa_K1422504 1 BBa_K1422504 Lactose-induced Killswitch using antiholin Hyb5 2014-10-16T11:00:00Z 2015-05-08T01:10:23Z Hyb5 comes from NCBI NCBI. (2 December, 2008). Lactobacillus phage YB5 holin gene, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/EF623894?report=GenBank This is a killswitch designed for Bacillus subtilis. It is lactose and IPTG inducible, has a RBS that has high efficiency with Bacillus subtilis, the antiholin Hyb5 as a lysing agent, and a forward terminator. Hyb5 was chosen because it is effective on Bacillus subtilis at neutral pH and 37 Celsius. It is also effective on certain strains of E. coli and other bacteria. Source: Wang, S.; Kong, J.; & Zhang, X. (19 November, 2008). Identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage ϕPYB5 of Lactobacillus fermentum. Journal of Applied Microbiology, Volume 105, Issue 6, pages 1939-1944. DOI: 10.1111/j.1365-2672.2008.03953.x http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03953.x/full false false _1800_ 0 21316 9 Not in stock false This killswitch is intended for Bacillus subtilis, and as such uses a RBS that works well in Bacillus. The choice of lactose as a promoter was chosen because it penetrates soil, is economically feasible to spread across a field, is not a food source for Bacillus, and does not harm plants or the natural soil microbiome. Hyb5 was chosen as a lysing agent because it is effective on Bacillus subtilis at neutral pH around field temperature. false Casey Martin annotation2429858 1 BBa_K143021 range2429858 1 3 12 annotation2429859 1 hyb5 range2429859 1 4 455 annotation2429857 1 PlaC range2429857 1 2 107 annotation2429860 1 BBa_B1006 range2429860 1 5 39 BBa_K1422504_sequence 1 agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaattaagcttaaaggtggtgaaatggaagtaaactcaattgcggacgtaatcacagcggtggcagtggcttcactaccaatcatcattacttacttgtctaagtggttaaagggcaaccgaacggctgaaactatcgtttcaatcttgccaactttagctaaggacgcggtcgtggccatgcaacaactcggggtggaaaaggctatcgaaggtgaagctaaaaagtcccatgccgtccagatcgtcaagcaagccttggctttaatgacacgaacgaagcgactttacagaatgcgatcgaagccgcctacgctcagcttaaggcagagggtaccttagacgcctacccacaagctaagccggcagacgacacgcaggccgaaattgctaaggctgaggcggctttagcgagcttgaaggagcaagaggccaccgctcaaaagcagcttgacgctttgaagggggcacagaattaaaaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z