LOCUS BBa_K1422505 3865 bp DNA linear 22-May-2024 DEFINITION Lactose-inducible killswitch, produces SAMS and NAS1 ACCESSION BBa_K1422505 VERSION BBa_K1422505.1 FEATURES Location/Qualifiers promoter 2..107 /label=PlaC RBS 3..12 /label=BBa_K143021 CDS 4..455 /label=hyb5 misc_feature 5..39 /label=BBa_B1006 misc_feature 1..613 /label=BBa_K1422504 promoter 684..689 /label=Sigma A-35 promoter 707..712 /label=Sigma A -10 promoter 622..718 /label=BBa_K143012 RBS 727..738 /label=rbs RBS 727..738 /label=BBa_K143021 misc_feature 747..2076 /label=BBa_K1422500 RBS 2085..2096 /label=rbs RBS 2085..2096 /label=BBa_K143021 misc_feature 2103..3086 /label=BBa_K1422501 RBS 3095..3106 /label=rbs RBS 3095..3106 /label=BBa_K143021 CDS 3113..3787 /label=mrfp1 misc_feature 3794..3818 /label=Help:Barcodes CDS 3113..3818 /label=BBa_E1010 misc_feature 3827..3865 /label=B1006 stem_loop 3836..3857 /label=modified thr terminator polyA_site 3858..3865 /label=PolyA polyA_site 3827..3835 /label=PolyA terminator 3827..3865 /label=BBa_B1006 ORIGIN 1 agaacaacct ctgctaaaat tcctgaaaaa ttttgcaaaa agttgttgac tttatctaca 61 aggtgtggca taatgtgtgg aattgtgagc ggataacaat taagcttaaa ggtggtgaaa 121 tggaagtaaa ctcaattgcg gacgtaatca cagcggtggc agtggcttca ctaccaatca 181 tcattactta cttgtctaag tggttaaagg gcaaccgaac ggctgaaact atcgtttcaa 241 tcttgccaac tttagctaag gacgcggtcg tggccatgca acaactcggg gtggaaaagg 301 ctatcgaagg tgaagctaaa aagtcccatg ccgtccagat cgtcaagcaa gccttggctt 361 taatgacacg aacgaagcga ctttacagaa tgcgatcgaa gccgcctacg ctcagcttaa 421 ggcagagggt accttagacg cctacccaca agctaagccg gcagacgaca cgcaggccga 481 aattgctaag gctgaggcgg ctttagcgag cttgaaggag caagaggcca ccgctcaaaa 541 gcagcttgac gctttgaagg gggcacagaa ttaaaaaaaa aaaccccgcc cctgacaggg 601 cggggttttt ttttactaga gaattttgtc aaaataattt tattgacaac gtcttattaa 661 cgttgatata atttaaattt tatttgacaa aaatgggctc gtgttgtaca ataaatgtta 721 ctagagaaag gtggtgaata ctagagcgtg ctacacaccc ttctccgttc gcttcttcac 781 cgccttcttg ccgtgtgttg aacaaagaac aacagcgtaa aagacttgta gaacgtgcac 841 gccgccgtca atggcgtcgt cgtgcttcat gcagcccaag aagtccgtga actcgtggaa 901 cacagacaag ctgtgcaaca cgctgtcgca ctcgctgttg gacgccggca tggcgtcgca 961 caccgacagc tagatggcct gcacgtccag caccgcgtcg tacatggtga tggtgttctg 1021 cacgtagccc gccaagacgc gcttggacga cacgcagatc ttgcgcgaca ccggcagcac 1081 gttctggctc accgccgaca acatggggat ctacacctac tgcagcgaga tgttggtgaa 1141 cctcctcatc ttcacctcct acgtctcgcc gcgcctgcac gggaacaagc cgctccggac 1201 cacgtcgtag cgcccctgca acacgtgcca cttgcagcgg aacgcctcct acgcgtcctc 1261 catcatgatg ccgctgagct acatgttggc ctcctagctc agcgcgtgca tgacgccgta 1321 gcgcgcgcac agcaccagct ccgggctctg gtcctacggc gcgccctcgt tgacgctggt 1381 ctacttgaac tcgcgcagca ccttggtgtc cttctgcttg cacaccgtgc agtagtcctc 1441 cgagcactac acgcccgtct cctacaacga gaagccctcc gacttcgcgt tcgacttcaa 1501 gctctccatc ttctcgtaga ggaacgtgga cgcggcgccc ttcatctacg agcacacgtc 1561 gcggcgctag cagctcagca ggccctacag gaactccggc ttctcctgca gcgcgttcct 1621 catctactcc tactgcggca ggtgaaccga cagcaggtgc gccttctcct gcgcgcacac 1681 cacctcgctg gacagcagca ggtccaacat cacctggacg cccacctcgc gcgtcttggc 1741 cggcggcgtc tccagctgct gcgtcttgcc gctgccctac tccgtcagca tgccgctctc 1801 cttgtccatg tagctctaca ccaactgcac cggggcccag ccctactaga cgttcttaac 1861 gttcatcacg tcgcacaagt acaagcggcc agggttggtc cccttcaaca tcgacttcac 1921 gtagagcagc aacaggcagc tcgagaccgc cgcatacagc tactttagca gtgacaacac 1981 ctacttcgcc gggccgttgg tctagtcctt aacgtcgtca tccgttaaag aacgtactgg 2041 gctcaagcct ggacatgcgt ttcattttcg tttggctact agagaaaggt ggtgaatact 2101 agatggaagc gcaaaacgtt gaagtagccg ctctcgtcca aaaaattgct gcattgcatg 2161 ccaatattac caaacttccg tctcttaatc catcaccaga cgcaaatgcc ctcttcactt 2221 ctcttgtgat ggcttgtgtg cctccgaatc ctgttgatgt aacaaaattg tctccggatg 2281 tccaaggaat gcgtgaagaa cttatccgcc tctgttctga cgcagaaggt cacctggaag 2341 cacattatgc tgacatgctt gctgccttcg ataacccgtt agaccactta ggacgcttcc 2401 catatttcag caattacatc gatttatcca aactggaatt cgaccttctt gtaagatata 2461 ttcctggtct ggctccgagc cgtgtagcat ttgttggatc tggtcctctg ccgttttctt 2521 ctcttgttct cgcagcgcgt catcttccta acacactttt cgataactac gatcgttgtg 2581 cggcagcaaa cgaccgcgcg agaaaattag ttcgcgcgga taaagactta aacgctcgca 2641 tgtcgtttca cacagtggat gtggctaact taactgatga actggctaaa tacgatgtag 2701 tatttttagc ggctcttgtt ggcatggcag ctgaagataa agcaaaagtc gtggcccacc 2761 ttggcagaca catggcggac ggcgcagcgt tagttgttcg ttctgcccac ggcgcgcgcg 2821 gattccttta tccaattgta gaccctgaag acatccgccg cggcggattc gatgttctgg 2881 ctgtttatca tccagataac gaagtgatta acagcgttat catcgcaaga aaaatggatg 2941 cacatacaaa aggattacag aatggtcacg ttcatgctcg tggcacagta ccgatcgtat 3001 ctccgccatg taaatgttgt aaaatggaag caaacgcatt acaaaaacgt gaagaaatgg 3061 caacaacgac agaattgtca atctgatact agagaaaggt ggtgaatact agatggcttc 3121 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa 3181 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac 3241 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc 3301 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct 3361 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg 3421 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa 3481 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg 3541 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat 3601 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc 3661 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc 3721 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac 3781 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagaaaa aaaaaccccg 3841 cccctgacag ggcggggttt ttttt //