LOCUS BBa_K1422506 4912 bp DNA linear 25-Nov-2024 DEFINITION Plasmid producing corn NAAT1, DMAS, and TOM1 ACCESSION BBa_K1422506 VERSION BBa_K1422506.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 126..1691 /label=BBa_K1422502 RBS 1700..1711 /label=rbs RBS 1700..1711 /label=BBa_K143021 misc_feature 1718..2662 /label=BBa_K1422503 RBS 2671..2682 /label=rbs RBS 2671..2682 /label=BBa_K143021 CDS 2689..4119 /label=BBa_K1422507 RBS 4128..4139 /label=rbs RBS 4128..4139 /label=BBa_K143021 misc_feature 4146..4148 /label=start CDS 4146..4865 /label=superfolder GFP coding region misc_feature 4860..4865 /label=stop CDS 4146..4865 /label=BBa_I746916 misc_feature 4874..4912 /label=B1006 stem_loop 4883..4904 /label=modified thr terminator polyA_site 4905..4912 /label=PolyA polyA_site 4874..4882 /label=PolyA terminator 4874..4912 /label=BBa_B1006 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagagtttga tccgacgcgc ccgaaccaat taaaaagctc aagtcctcaa cttgcccgtg 181 ctgcaaacgc acttcctcct gctagcacag cgcaaaatca cttgggaccg acaagctctt 241 taagacgccc gcttttcgtt cagcctatgg ctacacatgc ttctgacatt tggcagcgtg 301 ctcgcggcgt tgcagtgcgt tcaggaggtg aaggaggttg tgcaggacgc ggccgcggac 361 aagacgaaca tcctggcggt gcagtacaag accaacgcga acgtggccgt gcaagagctg 421 cggcacgcgc ggcggcttgc gcacgaggtc ctcttggtgt cccgggtctg ccgcaccgca 481 gacgcggacg cggtcgtcgt ggcagacgtg caccggatgg ccaagttcag ttactcccac 541 gtcgtcgtcg tccacctcgc tcccctcctt gttctggagg agcgccgtct tctgatttag 601 ctattcaggc agtaaattga tgacatcttc ctcacagatg gcgtcatagc tcacactgag 661 gatgtcgtct tagcccttgc cctacagggc gtcaccatat cgcaccgaaa acacgtcttt 721 ccaaactttg aggcgcatgc cgcactgcac aactgacatc ttcaccgttc cgtagcgatt 781 cttgagaacg tgttggtaac tgataccgtt tatctggtgt cgacctctga caagaacacc 841 atagaaacgg acaccataaa ccccaacaat cccttcgcca atgccttcat ccatgaacat 901 tcagccaagg acgtcgtggc tctaaagagg cttggaacac tagtcaccgt tgatgaagcg 961 tgtggaaacc aggagtttgg gaacaccctc ttcgtcctaa tggttgcttg tggccgcact 1021 gcagctgctt agagcatcgc attacgatcg aagagatgga ctcagcgctg gtagctacag 1081 gaatgggtag ctgcatgctt tctcagcaag actctagccg caaccaggat cattgtatta 1141 ataacaaact tccttaacgt cttaattgat ttagcaattt tcagggagct ttaccgccgt 1201 attactgaga acataaaccg aaaatcttta gcggcggaag ctttgtttgc tagcaaaaac 1261 cgcacccaat ctgctataaa tcgaataaag gccatcaact tcatcactta ccttcccagg 1321 cacgtcgctt tcatgtctgc cacggagaaa ttaaattcgt atcatttgga ggtgactctt 1381 gacgctactg atttttactt caagcaggta aacgtcgtgt atgtgacttt gtgtctagag 1441 aatgcttcgg aaatggtaag ttagatctcg ccagcttgtc tccgatcgat tcaatcttct 1501 catcttgatg gtcatgagaa gaccaaatct tcttacctaa agcgtaagaa gaagaacttc 1561 ttatggctat caattgtatt catgtacatg gatatcctgt ccctgtgttc ttcatcgtat 1621 cttccacaaa cgcagaatta tggaatccgc ctgaactcat caaattctca cttaacactc 1681 agaaaaaaaa atactagaga aaggtggtga atactagatg tcagcaacag gccgcgcacc 1741 ttgcggtctt cctcgcgtag gtcttggcac agctgtacaa ggtccgcgcc ctgaccctgt 1801 tcgcgcggcc gttttgcgtg cgatccagct tggataccgt cacttcgata ctgcggcaca 1861 ctatgcgaca gaagcgccta ttggtgaagc agctgcagaa gcagtacgca cgggattggt 1921 agcttctcgt gaagacttat tcgtgacttc caaagtttgg tgtgcggatg cccaccgtga 1981 tcgcgtcttg ccggctttac gtcgtacact ttcgaacctg caaatggaat atgttgattt 2041 gtacatggta cactggcctg taacaatgaa ggctggacgt ttcacagcac catttacacc 2101 tgaggatttt gaaccattcg atatgcgcgc agtttgggaa gcaatggaag agtgccaccg 2161 tctgggttta gctaaagcga ttggtgtttg caacttctct tgcaaaaaac ttgagacatt 2221 actttcattc gctactatcc ctcctgtcgt taaccaagtt gaaatcaacc cagtatggca 2281 acaacgtaaa cttcgcgaat tttgtagagc gaaaggcatt cagctttgcg catacagtcc 2341 gctgggtgct aagggaactc actggggatc tgattctgtt atggactctg gagttttgca 2401 cgagatcgcg aaatcaaaag gaaaaacagt agcgcaagtg tgtcttagat gggtgtatga 2461 acaaggcgat tgtttgattg tcaagtcttt cgatgaaggc cgtatgaaag agaacttaga 2521 tattgttgac tgggaactgt ctgaagagga aagacagcgt atttctaaaa ttccgcaacg 2581 caaaatcaat caaggtcgcc gttatgtctc tgaacacggt ccgtataaaa gctttgaaga 2641 gctgtgggca ggtgaaattt gatactagag aaaggtggtg aatactagat ggctgaagag 2701 gtcccgacta cccctccgcc tgcaccactc tacgtagacg gatgtcctgg ttgcgccatg 2761 gacagaaaga aagctgcaaa taaaggaatt ccttataaag aattcttttt tgtagctgta 2821 accacaattg cgtcagcatt gccaatctca agccttttcc cgttccttta tttcatgatt 2881 gaagatttac atgttgcaaa aaaagaacaa gatatcggcc tttacgcggg attccttgga 2941 gcatcttatt ttgtgggaag atttcttgtg tctttctttt ggggagtcgt tgctgaccgt 3001 gtgggtagaa aaccaattat tattttttcc gttgcatcag tcgtagtctt taacacactg 3061 ttcggtcttt cagtacaata ttggatggcg attactacta gacttctttt gggcgcactt 3121 aacggaatgc ttgctccgat taaagcatac tcaattgaga tctgccgccc tgaacatcat 3181 gcattgggac tctctgttgt ttccacagcg tggggcctcg gattaattgt aggtccatat 3241 attggtggtt acttagcgca acctgcacgc caatatccga atgttttttc cgaagaatct 3301 atttttggac gttttccgta tctgctgccg tgcctgagca ttagcatctt ttccgcggta 3361 gttcttgtta gctgcatttg gttaccagaa accctgcaca agcacaagaa cattgagaac 3421 gaggtaggca tgagcggcca ttctagaaca ccgcaaacgg aggacgtaga tgcgggaaaa 3481 tccttgtata aaaattggcc tctgatgagc tcaattatcg cttactgcgt gtttacactt 3541 cacgatacgg cgtatacgga aattctttcc ctttggacag tttccgacaa aaaatacggt 3601 ggccttggct tttcgtctaa aggcgtaggc caacttctcg ctattgctgg tctcggtctg 3661 attgtttatc agatgttcat ctatagacca gttcatagat accttggcag cgttaattcg 3721 tgccgcgctg cttcagcatt gagtattcca cttattgcag catttccgtt tatgacgcat 3781 ctttctggct ctaaattagg acttgctctg tatttcgcca caatcttaaa agctgcgctt 3841 ggaattacga ttttaactgg tacatcttta cttcaaaatt atgcagtgcc tcaacaccaa 3901 cgtggtgctg caaacggtgt agcggctaca gctatgagct ttttcaaagc tatcggccct 3961 gcgggtgcag gtgccttatt ttcatggacg caaaaaagac aagatgcagc ttttttccca 4021 ggagaccaca tgttattttt cattctgaac cttgtgcaat ttattggact tgctcttacg 4081 ttaaaaccgt ttctcgctgt acctaaacat tacaaatgat actagagaaa ggtggtgaat 4141 actagatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg 4201 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta 4261 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga 4321 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga 4381 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt 4441 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc 4501 tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc 4561 ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa 4621 atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg 4681 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc 4741 actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg 4801 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat 4861 gatgatacta gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tt //