BBa_K143012 1 Pveg Promoter veg a constitutive promoter for B. subtilis 2008-09-10T11:00:00Z 2015-05-08T01:10:23Z The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart. Released HQ 2013 Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor<cite>#1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes<cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how<cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator. false true _199_ 0 2090 9 In stock false The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence. false James Chappell annotation1975704 1 Sigma A-35 range1975704 1 63 68 annotation1975705 1 Sigma A -10 range1975705 1 86 91 BBa_K1422502 1 BBa_K1422502 Zea mays Nicotianamine aminotransferase (corn NAAT) 2014-10-12T11:00:00Z 2015-05-08T01:10:23Z The original sequence was obtained from the NCBI website. NCBI. (19 October, 2011). Zea mays ZmNAAT1 mRNA for putative nicotianamine aminotransferase, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/166788521 Nicotianamine aminotransferase (NAAT) catalases the conversion of nictotianamine to 3???deamino-3???-oxonicotianamine (a 3???-keto intermediate) and L-glutamate using 2-oxoglutarate. This is the third of four enzymes used to produce corn phytosiderophores and BBa_K1422500, BBa_K1422501, and BBa_K1422503 are also required to produce a phytosiderophore. Specifically, this is ZmNAAT1, although there are different forms among various species and types NAAT-A, NAAT-B, and NAAT1. Note: this sequence has been codon optimized for Bacillus subtilis. false false _1800_ 0 21316 9 Not in stock false Codon optimized for Bacillus subtilis by altering the 23rd, 109th, and 168th amino acid sequences from CTA to CTT. false Casey Martin BBa_K1422506 1 BBa_K1422506 Plasmid producing corn NAAT1, DMAS, and TOM1 2014-10-16T11:00:00Z 2015-05-08T01:10:23Z NAAT1: NCBI. (19 October, 2011). Zea mays ZmNAAT1 mRNA for putative nicotianamine aminotransferase, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/166788521 DMAS: NCBI. (2014). deoxymugineic acid synthase1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/162460852 TOM1: NCBI. (10 December, 2008). Zea mays clone 222622 protein transporter mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/195621011 This plasmid codes for NAAT1 (BBa_K1422502) and DMAS (BBa_K1422503), two of the four enzymes used to produce corn phytosiderophores; TOM1 (BBa_K1422507), which transports phytosiderophores out of cells; and sfGFP, as a reporter. BBa_K1422505 goes along with this plasmid, producing the other two enzymes necessary for the production of corn phytosiderophores, and includes a killswitch. false false _1800_ 0 21316 9 Not in stock false Using a conditional promoter so that the modified Bacillus subtilis would only produce phytosiderophores in the presence of plants roots was considered, but it was decided that conditional promoters would not be reliable enough. A constitutive promoter of medium strength was chosen for the corn genes because a weak promoter might not have produced enough phytosiderophores to make a difference, and a strong promoter might have overtaxed the cell in the production of phytosiderophores as to be counterproductive. The reporter is placed after the corn genes because gene expression decreases linearly as the distance from the promoter increases, and expressing the corn genes is much more important than expressing the reporter. sfGFP was used as a reporter because it is easily observed, easily measured, and expressed in Bacillus subtilis. The RBS SpoVG was chosen for its relative strength in Bacillus subtilis. A relatively strong terminator with few base pairs was selected for its relative effectiveness and low number of base pairs. NAAT can be coded by naat-A, naat-B, or naat-1; naat1 from corn was selected because a previous experiment revealed that the rice naat1 gene encodes functional NAAT and is expressed in cells of Fe-deficient leaves, strongly suggesting that it is involved in phytosiderophores production and sufficient to produce NAAT on its own, without other naat genes. false Casey Martin component2426827 1 BBa_I746916 component2426815 1 BBa_K1422502 component2426832 1 BBa_B1006 component2426814 1 BBa_K143021 component2426820 1 BBa_K143021 component2426823 1 BBa_K143021 component2426821 1 BBa_K1422507 component2426818 1 BBa_K1422503 component2426812 1 BBa_K143012 component2426817 1 BBa_K143021 annotation2426820 1 BBa_K143021 range2426820 1 2671 2682 annotation2426821 1 BBa_K1422507 range2426821 1 2689 4119 annotation2426823 1 BBa_K143021 range2426823 1 4128 4139 annotation2426815 1 BBa_K1422502 range2426815 1 126 1691 annotation2426817 1 BBa_K143021 range2426817 1 1700 1711 annotation2426814 1 BBa_K143021 range2426814 1 106 117 annotation2426812 1 BBa_K143012 range2426812 1 1 97 annotation2426827 1 BBa_I746916 range2426827 1 4146 4865 annotation2426818 1 BBa_K1422503 range2426818 1 1718 2662 annotation2426832 1 BBa_B1006 range2426832 1 4874 4912 BBa_K1422503 1 BBa_K1422503 Zea mays deoxymugineic acid synthase (corn DMAS) 2014-10-12T11:00:00Z 2015-05-08T01:10:23Z The sequence for this was obtained from the NCBI website. NCBI. (2014). deoxymugineic acid synthase1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/162460852 Deoxymugineic acid synthase (DMAS) reduces the 3"-carbon of 3???deamino-3???-oxonicotianamine (a 3???-keto intermediate) to produce 2???-Deoxymugineic Acid (DMA). This is the fourth of four enzymes used to produce corn phytosiderophores, and to produce a phytosiderophore would be used with BBa_K1422500, BBa_K1422501, and BBa_K1422502. false false _1800_ 0 21316 9 Not in stock false This sequence did not need to be altered to allow for Bacillus subtilis codon optimization. false Casey Martin BBa_I746916 1 BBa_I746916 superfolder GFP coding sequence 2008-09-29T11:00:00Z 2015-08-31T04:08:05Z Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart). This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven). false false _116_ 0 2122 9 It's complicated false Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis. false Stefan Milde annotation1977534 1 superfolder GFP coding region range1977534 1 1 720 annotation1977535 1 stop range1977535 1 715 720 annotation1977533 1 start range1977533 1 1 3 BBa_K143021 1 RBS-spoVG SpoVG ribosome binding site (RBS) for B. subtilis 2008-09-16T11:00:00Z 2015-05-08T01:10:23Z The sequence was taken from a previous research paper [1] and was constructed by Geneart. Released HQ 2013 Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite> false true _199_ 0 2090 9 In stock false In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained. false James Chappell annotation1975997 1 rbs range1975997 1 1 12 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 annotation1898431 1 PolyA range1898431 1 1 9 BBa_K1422507 1 BBa_K1422507 Zea mays TOM1 (corn phytosiderophore transporter) 2014-10-16T11:00:00Z 2015-05-08T01:10:23Z The original, unaltered gene sequence came from NCBI. NCBI. (10 December, 2008). Zea mays clone 222622 protein transporter mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/195621011 Zea mays TOM1 is a transporter protein for corn phytosiderophores, allowing them to exit corn cells. false false _1800_ 0 21316 9 Not in stock false Codon optimized for Bacillus subtilis. The 60th, 283rd, 293rd, and 295th codons were altered from CTA to CTT. false Casey Martin BBa_K1422506_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagagtttgatccgacgcgcccgaaccaattaaaaagctcaagtcctcaacttgcccgtgctgcaaacgcacttcctcctgctagcacagcgcaaaatcacttgggaccgacaagctctttaagacgcccgcttttcgttcagcctatggctacacatgcttctgacatttggcagcgtgctcgcggcgttgcagtgcgttcaggaggtgaaggaggttgtgcaggacgcggccgcggacaagacgaacatcctggcggtgcagtacaagaccaacgcgaacgtggccgtgcaagagctgcggcacgcgcggcggcttgcgcacgaggtcctcttggtgtcccgggtctgccgcaccgcagacgcggacgcggtcgtcgtggcagacgtgcaccggatggccaagttcagttactcccacgtcgtcgtcgtccacctcgctcccctccttgttctggaggagcgccgtcttctgatttagctattcaggcagtaaattgatgacatcttcctcacagatggcgtcatagctcacactgaggatgtcgtcttagcccttgccctacagggcgtcaccatatcgcaccgaaaacacgtctttccaaactttgaggcgcatgccgcactgcacaactgacatcttcaccgttccgtagcgattcttgagaacgtgttggtaactgataccgtttatctggtgtcgacctctgacaagaacaccatagaaacggacaccataaaccccaacaatcccttcgccaatgccttcatccatgaacattcagccaaggacgtcgtggctctaaagaggcttggaacactagtcaccgttgatgaagcgtgtggaaaccaggagtttgggaacaccctcttcgtcctaatggttgcttgtggccgcactgcagctgcttagagcatcgcattacgatcgaagagatggactcagcgctggtagctacaggaatgggtagctgcatgctttctcagcaagactctagccgcaaccaggatcattgtattaataacaaacttccttaacgtcttaattgatttagcaattttcagggagctttaccgccgtattactgagaacataaaccgaaaatctttagcggcggaagctttgtttgctagcaaaaaccgcacccaatctgctataaatcgaataaaggccatcaacttcatcacttaccttcccaggcacgtcgctttcatgtctgccacggagaaattaaattcgtatcatttggaggtgactcttgacgctactgatttttacttcaagcaggtaaacgtcgtgtatgtgactttgtgtctagagaatgcttcggaaatggtaagttagatctcgccagcttgtctccgatcgattcaatcttctcatcttgatggtcatgagaagaccaaatcttcttacctaaagcgtaagaagaagaacttcttatggctatcaattgtattcatgtacatggatatcctgtccctgtgttcttcatcgtatcttccacaaacgcagaattatggaatccgcctgaactcatcaaattctcacttaacactcagaaaaaaaaatactagagaaaggtggtgaatactagatgtcagcaacaggccgcgcaccttgcggtcttcctcgcgtaggtcttggcacagctgtacaaggtccgcgccctgaccctgttcgcgcggccgttttgcgtgcgatccagcttggataccgtcacttcgatactgcggcacactatgcgacagaagcgcctattggtgaagcagctgcagaagcagtacgcacgggattggtagcttctcgtgaagacttattcgtgacttccaaagtttggtgtgcggatgcccaccgtgatcgcgtcttgccggctttacgtcgtacactttcgaacctgcaaatggaatatgttgatttgtacatggtacactggcctgtaacaatgaaggctggacgtttcacagcaccatttacacctgaggattttgaaccattcgatatgcgcgcagtttgggaagcaatggaagagtgccaccgtctgggtttagctaaagcgattggtgtttgcaacttctcttgcaaaaaacttgagacattactttcattcgctactatccctcctgtcgttaaccaagttgaaatcaacccagtatggcaacaacgtaaacttcgcgaattttgtagagcgaaaggcattcagctttgcgcatacagtccgctgggtgctaagggaactcactggggatctgattctgttatggactctggagttttgcacgagatcgcgaaatcaaaaggaaaaacagtagcgcaagtgtgtcttagatgggtgtatgaacaaggcgattgtttgattgtcaagtctttcgatgaaggccgtatgaaagagaacttagatattgttgactgggaactgtctgaagaggaaagacagcgtatttctaaaattccgcaacgcaaaatcaatcaaggtcgccgttatgtctctgaacacggtccgtataaaagctttgaagagctgtgggcaggtgaaatttgatactagagaaaggtggtgaatactagatggctgaagaggtcccgactacccctccgcctgcaccactctacgtagacggatgtcctggttgcgccatggacagaaagaaagctgcaaataaaggaattccttataaagaattcttttttgtagctgtaaccacaattgcgtcagcattgccaatctcaagccttttcccgttcctttatttcatgattgaagatttacatgttgcaaaaaaagaacaagatatcggcctttacgcgggattccttggagcatcttattttgtgggaagatttcttgtgtctttcttttggggagtcgttgctgaccgtgtgggtagaaaaccaattattattttttccgttgcatcagtcgtagtctttaacacactgttcggtctttcagtacaatattggatggcgattactactagacttcttttgggcgcacttaacggaatgcttgctccgattaaagcatactcaattgagatctgccgccctgaacatcatgcattgggactctctgttgtttccacagcgtggggcctcggattaattgtaggtccatatattggtggttacttagcgcaacctgcacgccaatatccgaatgttttttccgaagaatctatttttggacgttttccgtatctgctgccgtgcctgagcattagcatcttttccgcggtagttcttgttagctgcatttggttaccagaaaccctgcacaagcacaagaacattgagaacgaggtaggcatgagcggccattctagaacaccgcaaacggaggacgtagatgcgggaaaatccttgtataaaaattggcctctgatgagctcaattatcgcttactgcgtgtttacacttcacgatacggcgtatacggaaattctttccctttggacagtttccgacaaaaaatacggtggccttggcttttcgtctaaaggcgtaggccaacttctcgctattgctggtctcggtctgattgtttatcagatgttcatctatagaccagttcatagataccttggcagcgttaattcgtgccgcgctgcttcagcattgagtattccacttattgcagcatttccgtttatgacgcatctttctggctctaaattaggacttgctctgtatttcgccacaatcttaaaagctgcgcttggaattacgattttaactggtacatctttacttcaaaattatgcagtgcctcaacaccaacgtggtgctgcaaacggtgtagcggctacagctatgagctttttcaaagctatcggccctgcgggtgcaggtgccttattttcatggacgcaaaaaagacaagatgcagcttttttcccaggagaccacatgttatttttcattctgaaccttgtgcaatttattggacttgctcttacgttaaaaccgtttctcgctgtacctaaacattacaaatgatactagagaaaggtggtgaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K1422503_sequence 1 atgtcagcaacaggccgcgcaccttgcggtcttcctcgcgtaggtcttggcacagctgtacaaggtccgcgccctgaccctgttcgcgcggccgttttgcgtgcgatccagcttggataccgtcacttcgatactgcggcacactatgcgacagaagcgcctattggtgaagcagctgcagaagcagtacgcacgggattggtagcttctcgtgaagacttattcgtgacttccaaagtttggtgtgcggatgcccaccgtgatcgcgtcttgccggctttacgtcgtacactttcgaacctgcaaatggaatatgttgatttgtacatggtacactggcctgtaacaatgaaggctggacgtttcacagcaccatttacacctgaggattttgaaccattcgatatgcgcgcagtttgggaagcaatggaagagtgccaccgtctgggtttagctaaagcgattggtgtttgcaacttctcttgcaaaaaacttgagacattactttcattcgctactatccctcctgtcgttaaccaagttgaaatcaacccagtatggcaacaacgtaaacttcgcgaattttgtagagcgaaaggcattcagctttgcgcatacagtccgctgggtgctaagggaactcactggggatctgattctgttatggactctggagttttgcacgagatcgcgaaatcaaaaggaaaaacagtagcgcaagtgtgtcttagatgggtgtatgaacaaggcgattgtttgattgtcaagtctttcgatgaaggccgtatgaaagagaacttagatattgttgactgggaactgtctgaagaggaaagacagcgtatttctaaaattccgcaacgcaaaatcaatcaaggtcgccgttatgtctctgaacacggtccgtataaaagctttgaagagctgtgggcaggtgaaatttga BBa_K143021_sequence 1 aaaggtggtgaa BBa_K1422507_sequence 1 atggctgaagaggtcccgactacccctccgcctgcaccactctacgtagacggatgtcctggttgcgccatggacagaaagaaagctgcaaataaaggaattccttataaagaattcttttttgtagctgtaaccacaattgcgtcagcattgccaatctcaagccttttcccgttcctttatttcatgattgaagatttacatgttgcaaaaaaagaacaagatatcggcctttacgcgggattccttggagcatcttattttgtgggaagatttcttgtgtctttcttttggggagtcgttgctgaccgtgtgggtagaaaaccaattattattttttccgttgcatcagtcgtagtctttaacacactgttcggtctttcagtacaatattggatggcgattactactagacttcttttgggcgcacttaacggaatgcttgctccgattaaagcatactcaattgagatctgccgccctgaacatcatgcattgggactctctgttgtttccacagcgtggggcctcggattaattgtaggtccatatattggtggttacttagcgcaacctgcacgccaatatccgaatgttttttccgaagaatctatttttggacgttttccgtatctgctgccgtgcctgagcattagcatcttttccgcggtagttcttgttagctgcatttggttaccagaaaccctgcacaagcacaagaacattgagaacgaggtaggcatgagcggccattctagaacaccgcaaacggaggacgtagatgcgggaaaatccttgtataaaaattggcctctgatgagctcaattatcgcttactgcgtgtttacacttcacgatacggcgtatacggaaattctttccctttggacagtttccgacaaaaaatacggtggccttggcttttcgtctaaaggcgtaggccaacttctcgctattgctggtctcggtctgattgtttatcagatgttcatctatagaccagttcatagataccttggcagcgttaattcgtgccgcgctgcttcagcattgagtattccacttattgcagcatttccgtttatgacgcatctttctggctctaaattaggacttgctctgtatttcgccacaatcttaaaagctgcgcttggaattacgattttaactggtacatctttacttcaaaattatgcagtgcctcaacaccaacgtggtgctgcaaacggtgtagcggctacagctatgagctttttcaaagctatcggccctgcgggtgcaggtgccttattttcatggacgcaaaaaagacaagatgcagcttttttcccaggagaccacatgttatttttcattctgaaccttgtgcaatttattggacttgctcttacgttaaaaccgtttctcgctgtacctaaacattacaaatga BBa_I746916_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga BBa_K143012_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K1422502_sequence 1 tttgatccgacgcgcccgaaccaattaaaaagctcaagtcctcaacttgcccgtgctgcaaacgcacttcctcctgctagcacagcgcaaaatcacttgggaccgacaagctctttaagacgcccgcttttcgttcagcctatggctacacatgcttctgacatttggcagcgtgctcgcggcgttgcagtgcgttcaggaggtgaaggaggttgtgcaggacgcggccgcggacaagacgaacatcctggcggtgcagtacaagaccaacgcgaacgtggccgtgcaagagctgcggcacgcgcggcggcttgcgcacgaggtcctcttggtgtcccgggtctgccgcaccgcagacgcggacgcggtcgtcgtggcagacgtgcaccggatggccaagttcagttactcccacgtcgtcgtcgtccacctcgctcccctccttgttctggaggagcgccgtcttctgatttagctattcaggcagtaaattgatgacatcttcctcacagatggcgtcatagctcacactgaggatgtcgtcttagcccttgccctacagggcgtcaccatatcgcaccgaaaacacgtctttccaaactttgaggcgcatgccgcactgcacaactgacatcttcaccgttccgtagcgattcttgagaacgtgttggtaactgataccgtttatctggtgtcgacctctgacaagaacaccatagaaacggacaccataaaccccaacaatcccttcgccaatgccttcatccatgaacattcagccaaggacgtcgtggctctaaagaggcttggaacactagtcaccgttgatgaagcgtgtggaaaccaggagtttgggaacaccctcttcgtcctaatggttgcttgtggccgcactgcagctgcttagagcatcgcattacgatcgaagagatggactcagcgctggtagctacaggaatgggtagctgcatgctttctcagcaagactctagccgcaaccaggatcattgtattaataacaaacttccttaacgtcttaattgatttagcaattttcagggagctttaccgccgtattactgagaacataaaccgaaaatctttagcggcggaagctttgtttgctagcaaaaaccgcacccaatctgctataaatcgaataaaggccatcaacttcatcacttaccttcccaggcacgtcgctttcatgtctgccacggagaaattaaattcgtatcatttggaggtgactcttgacgctactgatttttacttcaagcaggtaaacgtcgtgtatgtgactttgtgtctagagaatgcttcggaaatggtaagttagatctcgccagcttgtctccgatcgattcaatcttctcatcttgatggtcatgagaagaccaaatcttcttacctaaagcgtaagaagaagaacttcttatggctatcaattgtattcatgtacatggatatcctgtccctgtgttcttcatcgtatcttccacaaacgcagaattatggaatccgcctgaactcatcaaattctcacttaacactcagaaaaaaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z