BBa_K1422506
type: ComponentDefinition
persistent identity: BBa K1422506
display id: BBa_K1422506
version: 1
was generated by: iGEM to SBOL conversion
description: Plasmid producing corn NAAT1, DMAS, and TOM1
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: NAAT1: NCBI. (19 October, 2011). Zea mays ZmNAAT1 mRNA for putative nicotianamine aminotransferase, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/166788521 DMAS: NCBI. (2014). deoxymugineic acid synthase1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/162460852 TOM1: NCBI. (10 December, 2008). Zea mays clone 222622 protein transporter mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/195621011
owned by: james
owned by: myers
mutable description: This plasmid codes for NAAT1 (BBa_K1422502) and DMAS (BBa_K1422503), two of the four enzymes used to produce corn phytosiderophores; TOM1 (BBa_K1422507), which transports phytosiderophores out of cells; and sfGFP, as a reporter. BBa_K1422505 goes along with this plasmid, producing the other two enzymes necessary for the production of corn phytosiderophores, and includes a killswitch.
discontinued: false
dominant: false
group u list: _1800_
m user id: 0
owner id: 21316
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Using a conditional promoter so that the modified Bacillus subtilis would only produce phytosiderophores in the presence of plants roots was considered, but it was decided that conditional promoters would not be reliable enough. A constitutive promoter of medium strength was chosen for the corn genes because a weak promoter might not have produced enough phytosiderophores to make a difference, and a strong promoter might have overtaxed the cell in the production of phytosiderophores as to be counterproductive. The reporter is placed after the corn genes because gene expression decreases linearly as the distance from the promoter increases, and expressing the corn genes is much more important than expressing the reporter. sfGFP was used as a reporter because it is easily observed, easily measured, and expressed in Bacillus subtilis. The RBS SpoVG was chosen for its relative strength in Bacillus subtilis. A relatively strong terminator with few base pairs was selected for its relative effectiveness and low number of base pairs. NAAT can be coded by naat-A, naat-B, or naat-1; naat1 from corn was selected because a previous experiment revealed that the rice naat1 gene encodes functional NAAT and is expressed in cells of Fe-deficient leaves, strongly suggesting that it is involved in phytosiderophores production and sufficient to produce NAAT on its own, without other naat genes.
star: false
creator: Casey Martin
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B1006
component: BBa_I746916
component: BBa_K143021
component: BBa_K1422507
component: BBa_K1422503
component: BBa_K1422502
component: BBa_K143012
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence annotation: BBa_K1422502
sequence annotation: BBa_K1422503
sequence annotation: BBa_K1422507
sequence annotation: BBa_I746916
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1422506_sequence/1

BBa_B1006
type: Component
persistent identity: component2426832
display id: component2426832
version: 1
top level: BBa_K1422506
definition: BBa_B1006
access: public

BBa_I746916
type: Component
persistent identity: component2426827
display id: component2426827
version: 1
top level: BBa_K1422506
definition: BBa_I746916
access: public

BBa_K143021
type: Component
persistent identity: component2426823
display id: component2426823
version: 1
top level: BBa_K1422506
definition: RBS-spoVG
access: public

BBa_K1422507
type: Component
persistent identity: component2426821
display id: component2426821
version: 1
top level: BBa_K1422506
definition: BBa_K1422507
access: public

BBa_K143021
type: Component
persistent identity: component2426820
display id: component2426820
version: 1
top level: BBa_K1422506
definition: RBS-spoVG
access: public

BBa_K1422503
type: Component
persistent identity: component2426818
display id: component2426818
version: 1
top level: BBa_K1422506
definition: BBa_K1422503
access: public

BBa_K143021
type: Component
persistent identity: component2426817
display id: component2426817
version: 1
top level: BBa_K1422506
definition: RBS-spoVG
access: public

BBa_K1422502
type: Component
persistent identity: component2426815
display id: component2426815
version: 1
top level: BBa_K1422506
definition: BBa_K1422502
access: public

BBa_K143021
type: Component
persistent identity: component2426814
display id: component2426814
version: 1
top level: BBa_K1422506
definition: RBS-spoVG
access: public

BBa_K143012
type: Component
persistent identity: component2426812
display id: component2426812
version: 1
top level: BBa_K1422506
definition: Pveg
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2426812
display id: annotation2426812
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426812/range2426812/1
component: BBa_K143012

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persistent identity: range2426812
display id: range2426812
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 1
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orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2426814
display id: annotation2426814
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426814/range2426814/1
component: BBa_K143021

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type: Range
persistent identity: range2426814
display id: range2426814
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 106
end: 117
orientation: inline

BBa_K1422502
type: SequenceAnnotation
persistent identity: annotation2426815
display id: annotation2426815
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426815/range2426815/1
component: BBa_K1422502

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type: Range
persistent identity: range2426815
display id: range2426815
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 126
end: 1691
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2426817
display id: annotation2426817
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426817/range2426817/1
component: BBa_K143021

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type: Range
persistent identity: range2426817
display id: range2426817
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 1700
end: 1711
orientation: inline

BBa_K1422503
type: SequenceAnnotation
persistent identity: annotation2426818
display id: annotation2426818
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426818/range2426818/1
component: BBa_K1422503

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type: Range
persistent identity: range2426818
display id: range2426818
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 1718
end: 2662
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2426820
display id: annotation2426820
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426820/range2426820/1
component: BBa_K143021

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type: Range
persistent identity: range2426820
display id: range2426820
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 2671
end: 2682
orientation: inline

BBa_K1422507
type: SequenceAnnotation
persistent identity: annotation2426821
display id: annotation2426821
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426821/range2426821/1
component: BBa_K1422507

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persistent identity: range2426821
display id: range2426821
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 2689
end: 4119
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BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2426823
display id: annotation2426823
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426823/range2426823/1
component: BBa_K143021

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persistent identity: range2426823
display id: range2426823
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 4128
end: 4139
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BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2426827
display id: annotation2426827
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426827/range2426827/1
component: BBa_I746916

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persistent identity: range2426827
display id: range2426827
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 4146
end: 4865
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2426832
display id: annotation2426832
version: 1
top level: BBa_K1422506
location: https://synbiohub.org/public/igem/BBa_K1422506/annotation2426832/range2426832/1
component: BBa_B1006

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type: Range
persistent identity: range2426832
display id: range2426832
version: 1
top level: BBa_K1422506
direction: direction/reverse
start: 4874
end: 4912
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP coding sequence
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
star: false
creator: Stefan Milde
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: superfolder GFP coding region
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
role: feature/protein
role: SO:0000316

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type: Range
persistent identity: range1977534
display id: range1977534
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range1977533
display id: range1977533
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range1977535
display id: range1977535
version: 1
top level: BBa_I746916
direction: direction/forward
start: 715
end: 720
orientation: inline

BBa_K1422507
type: ComponentDefinition
persistent identity: BBa K1422507
display id: BBa_K1422507
version: 1
was generated by: iGEM to SBOL conversion
description: Zea mays TOM1 (corn phytosiderophore transporter)
created: 2014-10-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The original, unaltered gene sequence came from NCBI. NCBI. (10 December, 2008). Zea mays clone 222622 protein transporter mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/195621011
owned by: james
owned by: myers
mutable description: Zea mays TOM1 is a transporter protein for corn phytosiderophores, allowing them to exit corn cells.
discontinued: false
dominant: false
group u list: _1800_
m user id: 0
owner id: 21316
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for Bacillus subtilis. The 60th, 283rd, 293rd, and 295th codons were altered from CTA to CTT.
star: false
creator: Casey Martin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1422507_sequence/1

BBa_K1422502
type: ComponentDefinition
persistent identity: BBa K1422502
display id: BBa_K1422502
version: 1
was generated by: iGEM to SBOL conversion
description: Zea mays Nicotianamine aminotransferase (corn NAAT)
created: 2014-10-12T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The original sequence was obtained from the NCBI website. NCBI. (19 October, 2011). Zea mays ZmNAAT1 mRNA for putative nicotianamine aminotransferase, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/166788521
owned by: james
owned by: myers
mutable description: Nicotianamine aminotransferase (NAAT) catalases the conversion of nictotianamine to 3???deamino-3???-oxonicotianamine (a 3???-keto intermediate) and L-glutamate using 2-oxoglutarate. This is the third of four enzymes used to produce corn phytosiderophores and BBa_K1422500, BBa_K1422501, and BBa_K1422503 are also required to produce a phytosiderophore. Specifically, this is ZmNAAT1, although there are different forms among various species and types NAAT-A, NAAT-B, and NAAT1. Note: this sequence has been codon optimized for Bacillus subtilis.
discontinued: false
dominant: false
group u list: _1800_
m user id: 0
owner id: 21316
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for Bacillus subtilis by altering the 23rd, 109th, and 168th amino acid sequences from CTA to CTT.
star: false
creator: Casey Martin
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1422502_sequence/1

BBa_K1422503
type: ComponentDefinition
persistent identity: BBa K1422503
display id: BBa_K1422503
version: 1
was generated by: iGEM to SBOL conversion
description: Zea mays deoxymugineic acid synthase (corn DMAS)
created: 2014-10-12T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence for this was obtained from the NCBI website. NCBI. (2014). deoxymugineic acid synthase1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/162460852
owned by: james
owned by: myers
mutable description: Deoxymugineic acid synthase (DMAS) reduces the 3"-carbon of 3???deamino-3???-oxonicotianamine (a 3???-keto intermediate) to produce 2???-Deoxymugineic Acid (DMA). This is the fourth of four enzymes used to produce corn phytosiderophores, and to produce a phytosiderophore would be used with BBa_K1422500, BBa_K1422501, and BBa_K1422502.
discontinued: false
dominant: false
group u list: _1800_
m user id: 0
owner id: 21316
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence did not need to be altered to allow for Bacillus subtilis codon optimization.
star: false
creator: Casey Martin
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1422503_sequence/1

https://synbiohub.org/public/igem/BBa_K1422506_sequence/1
type: Sequence
persistent identity: BBa K1422506 sequence
display id: BBa_K1422506_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagagtttgatccgacgcgcccgaaccaattaaaaagctcaagtcctcaacttgcccgtgctgcaaacgcacttcctcctgctagcacagcgcaaaatcacttgggaccgacaagctctttaagacgcccgcttttcgttcagcctatggctacacatgcttctgacatttggcagcgtgctcgcggcgttgcagtgcgttcaggaggtgaaggaggttgtgcaggacgcggccgcggacaagacgaacatcctggcggtgcagtacaagaccaacgcgaacgtggccgtgcaagagctgcggcacgcgcggcggcttgcgcacgaggtcctcttggtgtcccgggtctgccgcaccgcagacgcggacgcggtcgtcgtggcagacgtgcaccggatggccaagttcagttactcccacgtcgtcgtcgtccacctcgctcccctccttgttctggaggagcgccgtcttctgatttagctattcaggcagtaaattgatgacatcttcctcacagatggcgtcatagctcacactgaggatgtcgtcttagcccttgccctacagggcgtcaccatatcgcaccgaaaacacgtctttccaaactttgaggcgcatgccgcactgcacaactgacatcttcaccgttccgtagcgattcttgagaacgtgttggtaactgataccgtttatctggtgtcgacctctgacaagaacaccatagaaacggacaccataaaccccaacaatcccttcgccaatgccttcatccatgaacattcagccaaggacgtcgtggctctaaagaggcttggaacactagtcaccgttgatgaagcgtgtggaaaccaggagtttgggaacaccctcttcgtcctaatggttgcttgtggccgcactgcagctgcttagagcatcgcattacgatcgaagagatggactcagcgctggtagctacaggaatgggtagctgcatgctttctcagcaagactctagccgcaaccaggatcattgtattaataacaaacttccttaacgtcttaattgatttagcaattttcagggagctttaccgccgtattactgagaacataaaccgaaaatctttagcggcggaagctttgtttgctagcaaaaaccgcacccaatctgctataaatcgaataaaggccatcaacttcatcacttaccttcccaggcacgtcgctttcatgtctgccacggagaaattaaattcgtatcatttggaggtgactcttgacgctactgatttttacttcaagcaggtaaacgtcgtgtatgtgactttgtgtctagagaatgcttcggaaatggtaagttagatctcgccagcttgtctccgatcgattcaatcttctcatcttgatggtcatgagaagaccaaatcttcttacctaaagcgtaagaagaagaacttcttatggctatcaattgtattcatgtacatggatatcctgtccctgtgttcttcatcgtatcttccacaaacgcagaattatggaatccgcctgaactcatcaaattctcacttaacactcagaaaaaaaaatactagagaaaggtggtgaatactagatgtcagcaacaggccgcgcaccttgcggtcttcctcgcgtaggtcttggcacagctgtacaaggtccgcgccctgaccctgttcgcgcggccgttttgcgtgcgatccagcttggataccgtcacttcgatactgcggcacactatgcgacagaagcgcctattggtgaagcagctgcagaagcagtacgcacgggattggtagcttctcgtgaagacttattcgtgacttccaaagtttggtgtgcggatgcccaccgtgatcgcgtcttgccggctttac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746916_sequence/1
type: Sequence
persistent identity: BBa I746916 sequence
display id: BBa_I746916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1422507_sequence/1
type: Sequence
persistent identity: BBa K1422507 sequence
display id: BBa_K1422507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagaggtcccgactacccctccgcctgcaccactctacgtagacggatgtcctggttgcgccatggacagaaagaaagctgcaaataaaggaattccttataaagaattcttttttgtagctgtaaccacaattgcgtcagcattgccaatctcaagccttttcccgttcctttatttcatgattgaagatttacatgttgcaaaaaaagaacaagatatcggcctttacgcgggattccttggagcatcttattttgtgggaagatttcttgtgtctttcttttggggagtcgttgctgaccgtgtgggtagaaaaccaattattattttttccgttgcatcagtcgtagtctttaacacactgttcggtctttcagtacaatattggatggcgattactactagacttcttttgggcgcacttaacggaatgcttgctccgattaaagcatactcaattgagatctgccgccctgaacatcatgcattgggactctctgttgtttccacagcgtggggcctcggattaattgtaggtccatatattggtggttacttagcgcaacctgcacgccaatatccgaatgttttttccgaagaatctatttttggacgttttccgtatctgctgccgtgcctgagcattagcatcttttccgcggtagttcttgttagctgcatttggttaccagaaaccctgcacaagcacaagaacattgagaacgaggtaggcatgagcggccattctagaacaccgcaaacggaggacgtagatgcgggaaaatccttgtataaaaattggcctctgatgagctcaattatcgcttactgcgtgtttacacttcacgatacggcgtatacggaaattctttccctttggacagtttccgacaaaaaatacggtggccttggcttttcgtctaaaggcgtaggccaacttctcgctattgctggtctcggtctgattgtttatcagatgttcatctatagaccagttcatagataccttggcagcgttaattcgtgccgcgctgcttcagcattgagtattccacttattgcagcatttccgtttatgacgcatctttctggctctaaattaggacttgctctgtatttcgccacaatcttaaaagctgcgcttggaattacgattttaactggtacatctttacttcaaaattatgcagtgcctcaacaccaacgtggtgctgcaaacggtgtagcggctacagctatgagctttttcaaagctatcggccctgcgggtgcaggtgccttattttcatggacgcaaaaaagacaagatgcagcttttttcccaggagaccacatgttatttttcattctgaaccttgtgcaatttattggacttgctcttacgttaaaaccgtttctcgctgtacctaaacattacaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1422502_sequence/1
type: Sequence
persistent identity: BBa K1422502 sequence
display id: BBa_K1422502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttgatccgacgcgcccgaaccaattaaaaagctcaagtcctcaacttgcccgtgctgcaaacgcacttcctcctgctagcacagcgcaaaatcacttgggaccgacaagctctttaagacgcccgcttttcgttcagcctatggctacacatgcttctgacatttggcagcgtgctcgcggcgttgcagtgcgttcaggaggtgaaggaggttgtgcaggacgcggccgcggacaagacgaacatcctggcggtgcagtacaagaccaacgcgaacgtggccgtgcaagagctgcggcacgcgcggcggcttgcgcacgaggtcctcttggtgtcccgggtctgccgcaccgcagacgcggacgcggtcgtcgtggcagacgtgcaccggatggccaagttcagttactcccacgtcgtcgtcgtccacctcgctcccctccttgttctggaggagcgccgtcttctgatttagctattcaggcagtaaattgatgacatcttcctcacagatggcgtcatagctcacactgaggatgtcgtcttagcccttgccctacagggcgtcaccatatcgcaccgaaaacacgtctttccaaactttgaggcgcatgccgcactgcacaactgacatcttcaccgttccgtagcgattcttgagaacgtgttggtaactgataccgtttatctggtgtcgacctctgacaagaacaccatagaaacggacaccataaaccccaacaatcccttcgccaatgccttcatccatgaacattcagccaaggacgtcgtggctctaaagaggcttggaacactagtcaccgttgatgaagcgtgtggaaaccaggagtttgggaacaccctcttcgtcctaatggttgcttgtggccgcactgcagctgcttagagcatcgcattacgatcgaagagatggactcagcgctggtagctacaggaatgggtagctgcatgctttctcagcaagactctagccgcaaccaggatcattgtattaataacaaacttccttaacgtcttaattgatttagcaattttcagggagctttaccgccgtattactgagaacataaaccgaaaatctttagcggcggaagctttgtttgctagcaaaaaccgcacccaatctgctataaatcgaataaaggccatcaacttcatcacttaccttcccaggcacgtcgctttcatgtctgccacggagaaattaaattcgtatcatttggaggtgactcttgacgctactgatttttacttcaagcaggtaaacgtcgtgtatgtgactttgtgtctagagaatgcttcggaaatggtaagttagatctcgccagcttgtctccgatcgattcaatcttctcatcttgatggtcatgagaagaccaaatcttcttacctaaagcgtaagaagaagaacttcttatggctatcaattgtattcatgtacatggatatcctgtccctgtgttcttcatcgtatcttccacaaacgcagaattatggaatccgcctgaactcatcaaattctcacttaacactcagaaaaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1422503_sequence/1
type: Sequence
persistent identity: BBa K1422503 sequence
display id: BBa_K1422503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcagcaacaggccgcgcaccttgcggtcttcctcgcgtaggtcttggcacagctgtacaaggtccgcgccctgaccctgttcgcgcggccgttttgcgtgcgatccagcttggataccgtcacttcgatactgcggcacactatgcgacagaagcgcctattggtgaagcagctgcagaagcagtacgcacgggattggtagcttctcgtgaagacttattcgtgacttccaaagtttggtgtgcggatgcccaccgtgatcgcgtcttgccggctttacgtcgtacactttcgaacctgcaaatggaatatgttgatttgtacatggtacactggcctgtaacaatgaaggctggacgtttcacagcaccatttacacctgaggattttgaaccattcgatatgcgcgcagtttgggaagcaatggaagagtgccaccgtctgggtttagctaaagcgattggtgtttgcaacttctcttgcaaaaaacttgagacattactttcattcgctactatccctcctgtcgttaaccaagttgaaatcaacccagtatggcaacaacgtaaacttcgcgaattttgtagagcgaaaggcattcagctttgcgcatacagtccgctgggtgctaagggaactcactggggatctgattctgttatggactctggagttttgcacgagatcgcgaaatcaaaaggaaaaacagtagcgcaagtgtgtcttagatgggtgtatgaacaaggcgattgtttgattgtcaagtctttcgatgaaggccgtatgaaagagaacttagatattgttgactgggaactgtctgaagaggaaagacagcgtatttctaaaattccgcaacgcaaaatcaatcaaggtcgccgttatgtctctgaacacggtccgtataaaagctttgaagagctgtgggcaggtgaaatttga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z