BBa_K1423002 1 BBa_K1423002 BCLA N-terminal domain and CAEV p-28 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z Bacillus anthracis and caprine arthritis encephalitis virus This part is a cell surface anchoring motif with a caprine arthritis encephalitis virus capsid protein domain. The capsid protein domain acts as an antigen in sheeps and goats. false false _1801_ 0 22751 9 It's complicated false This part was an intermediate, but we needed to ensure that the CAEV capsid protein domain was in frame once it was attached to the BCLA NTD. false Corbyn Lamy component2383223 1 BBa_K1423000 component2383224 1 BBa_K1423001 annotation2383223 1 BBa_K1423000 range2383223 1 1 63 annotation2383224 1 BBa_K1423001 range2383224 1 72 674 BBa_K1423000 1 BBa_K1423000 BCLA N-terminal Domain 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z Bacillus anthracis This part is the N-terminal domain of BCLA, and it can be used as an anchoring motif for cell surface display. BCLA is a hair-like glycoprotein found on the outermost layer of the Bacillus anthracis spore. It consists of three parts: an N terminal domain, a central domain (also called collagen-like region ???CLR???), and a C terminal domain. A picture of the structure of BCLA can be seen below. false false _1801_ 0 22136 9 In stock false When designing the part we ensured that BCLA and any following parts would remain in frame. This is essential because as an anchoring motif we expect additional domains to be attached after. false Shawna Henry BBa_K1423001 1 BBa_K1423001 CAEV Capsid protein p-28 domain 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z This part is from the caprine arthritis encephalitis virus. Caprine arthritis encephalitis virus infects goats and sheep. The p-28 domain is from the capsid protein of the virus. Within CAEV the p-28 domain is an antigen recognized by goat and sheep antibodies. In our work we used this domain for antigen-antibody pairing for agglutination assays. false false _1801_ 0 22751 9 In stock false In order to make sure we did not have a full viral protein we isolated a single domain from the capsid protein of the caprine arthritis encephalitis virus. false Corbyn Lamy BBa_K1423001_sequence 1 ttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataa BBa_K1423000_sequence 1 gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaaca BBa_K1423002_sequence 1 gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaacatactagagttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z