BBa_K1423002
1
BBa_K1423002
BCLA N-terminal domain and CAEV p-28
2014-09-12T11:00:00Z
2015-05-08T01:10:23Z
Bacillus anthracis and caprine arthritis encephalitis virus
This part is a cell surface anchoring motif with a caprine arthritis encephalitis virus capsid protein domain. The capsid protein domain acts as an antigen in sheeps and goats.
false
false
_1801_
0
22751
9
It's complicated
false
This part was an intermediate, but we needed to ensure that the CAEV capsid protein domain was in frame once it was attached to the BCLA NTD.
false
Corbyn Lamy
component2383223
1
BBa_K1423000
component2383224
1
BBa_K1423001
annotation2383223
1
BBa_K1423000
range2383223
1
1
63
annotation2383224
1
BBa_K1423001
range2383224
1
72
674
BBa_K1423000
1
BBa_K1423000
BCLA N-terminal Domain
2014-09-12T11:00:00Z
2015-05-08T01:10:23Z
Bacillus anthracis
This part is the N-terminal domain of BCLA, and it can be used as an anchoring motif for cell surface display. BCLA is a hair-like glycoprotein found on the outermost layer of the Bacillus anthracis spore. It consists of three parts: an N terminal domain, a central domain (also called collagen-like region ???CLR???), and a C terminal domain. A picture of the structure of BCLA can be seen below.
false
false
_1801_
0
22136
9
In stock
false
When designing the part we ensured that BCLA and any following parts would remain in frame. This is essential because as an anchoring motif we expect additional domains to be attached after.
false
Shawna Henry
BBa_K1423001
1
BBa_K1423001
CAEV Capsid protein p-28 domain
2014-09-12T11:00:00Z
2015-05-08T01:10:23Z
This part is from the caprine arthritis encephalitis virus.
Caprine arthritis encephalitis virus infects goats and sheep. The p-28 domain is from the capsid protein of the virus. Within CAEV the p-28 domain is an antigen recognized by goat and sheep antibodies. In our work we used this domain for antigen-antibody pairing for agglutination assays.
false
false
_1801_
0
22751
9
In stock
false
In order to make sure we did not have a full viral protein we isolated a single domain from the capsid protein of the caprine arthritis encephalitis virus.
false
Corbyn Lamy
BBa_K1423001_sequence
1
ttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataa
BBa_K1423000_sequence
1
gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaaca
BBa_K1423002_sequence
1
gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaacatactagagttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z