BBa_K1423004 1 BBa_K1423004 CAEV capsid protein anchored in cell membrane 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z bacillus anthracis and caprine arthritis encephalitis virus This part is a constitutive promoter, a ribosome binding site, a cell surface anchoring motif, a caprine arthritis encephalitis virus capsid protein domain, and a double terminator. We designed this part to use in our direct agglutination assay. The BCLA NTD will allow the capsid protein domain to be expressed on the outer surface of the cell. The capsid protein domain is an antigen which we then used with antibodies for agglutination. false false _1801_ 0 22751 9 It's complicated false The CAEV capsid protein needed to be in frame next to the BCLA domain. false Corbyn Lamy component2383228 1 BBa_K1423000 component2383229 1 BBa_K1423001 component2383225 1 BBa_J23100 component2383227 1 BBa_B0034 component2383236 1 BBa_B0015 annotation2383227 1 BBa_B0034 range2383227 1 44 55 annotation2383229 1 BBa_K1423001 range2383229 1 135 737 annotation2383225 1 BBa_J23100 range2383225 1 1 35 annotation2383236 1 BBa_B0015 range2383236 1 746 874 annotation2383228 1 BBa_K1423000 range2383228 1 64 126 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1423000 1 BBa_K1423000 BCLA N-terminal Domain 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z Bacillus anthracis This part is the N-terminal domain of BCLA, and it can be used as an anchoring motif for cell surface display. BCLA is a hair-like glycoprotein found on the outermost layer of the Bacillus anthracis spore. It consists of three parts: an N terminal domain, a central domain (also called collagen-like region ???CLR???), and a C terminal domain. A picture of the structure of BCLA can be seen below. false false _1801_ 0 22136 9 In stock false When designing the part we ensured that BCLA and any following parts would remain in frame. This is essential because as an anchoring motif we expect additional domains to be attached after. false Shawna Henry BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1423001 1 BBa_K1423001 CAEV Capsid protein p-28 domain 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z This part is from the caprine arthritis encephalitis virus. Caprine arthritis encephalitis virus infects goats and sheep. The p-28 domain is from the capsid protein of the virus. Within CAEV the p-28 domain is an antigen recognized by goat and sheep antibodies. In our work we used this domain for antigen-antibody pairing for agglutination assays. false false _1801_ 0 22751 9 In stock false In order to make sure we did not have a full viral protein we isolated a single domain from the capsid protein of the caprine arthritis encephalitis virus. false Corbyn Lamy BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1423001_sequence 1 ttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataa BBa_K1423004_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagaggcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaacatactagagttccagcaactgcaaacagtagcaatgcagcatggcctcgtgtctgaggactttgaaaggcagttggcatattatgctactacctggacaagtaaagacatactagaagtattggccatgatgcctggaaatagagctcaaaaggagttaattcaagggaaattaaatgaagaagcagaaaggtggagaaggaataatccaccacctccagcaggaggaggattaacagtggatcaaattatgggggtaggacaaacaaatcaagcagcagcacaagctaacatggatcaggcaaggcaaatatgcctgcaatgggtaataaatgcattaagagcagtaagacatatggcgcacaggccagggaatccaatgctagtaaagcaaaaaacgaatgagccatatgaagattttgcagcaagactgctagaagcaatagatgcagagccagttacacagcctataaaagattatctaaagctaacactatcttatacaaatgcatcagcagattgtcagaagcaaatggatagaacactaggacaaagagtacaacaagctagtgtagaagaaaaaatgcaagcatgtagagatgtgggataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1423000_sequence 1 gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaaca BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z