LOCUS       BBa_K1423006            2265 bp    DNA     linear       22-Nov-2024
DEFINITION  Arsenic Inducible Banana Odor Generator
ACCESSION   BBa_K1423006
VERSION     BBa_K1423006.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     RBS             527..541
                     /label=RBS-1\Strong
     RBS             534..538
                     /label=RBS
     misc_feature    527..541
                     /label=BBa_B0030
     RBS             527..541
                     /label=BBa_B0030
     CDS             548..2128
                     /label=ATF1
     misc_feature    1695..1695
                     /label=t -> c
     misc_feature    2123..2128
                     /label=double TAA stop codon
     misc_feature    961..961
                     /label=a->g to eliminate EcoRI site
     CDS             548..2128
                     /label=BBa_J45014
     stem_loop       2148..2191
                     /label=stem_loop
     misc_feature    2137..2216
                     /label=BBa_B0010
     terminator      2137..2216
                     /label=BBa_B0010
     stem_loop       2232..2251
                     /label=T7 TE
     misc_feature    2258..2258
                     /label=stop
     polyA_site      2252..2265
                     /label=polya
     misc_feature    2225..2265
                     /label=BBa_B0012
     terminator      2225..2265
                     /label=BBa_B0012
     misc_feature    527..2265
                     /label=BBa_J45199
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagatta aagaggagaa
      541 atactagatg aatgaaatcg atgagaaaaa tcaggccccc gtgcaacaag aatgcctgaa
      601 agagatgatt cagaatgggc atgctcggcg tatgggatct gttgaagatc tgtatgttgc
      661 tctcaacaga caaaacttat atcgaaactt ctgcacatat ggagaattga gtgattactg
      721 tactagggat cagctcacat tagctttgag ggaaatctgc ctgaaaaatc caactctttt
      781 acatattgtt ctaccaacaa gatggccaaa tcatgaaaat tattatcgca gttccgaata
      841 ctattcacgg ccacatccag tgcatgatta tatttcagta ttacaagaat tgaaactgag
      901 tggtgtggtt ctcaatgaac aacctgagta cagtgcagta atgaagcaaa tattagaaga
      961 gttcaaaaat agtaagggtt cctatactgc aaaaattttt aaacttacta ccactttgac
     1021 tattccttac tttggaccaa caggaccgag ttggcggcta atttgtcttc cagaagagca
     1081 cacagaaaag tggaaaaaat ttatctttgt atctaatcat tgcatgtctg atggtcggtc
     1141 ttcgatccac ttttttcatg atttaagaga cgaattaaat aatattaaaa ctccaccaaa
     1201 aaaattagat tacattttca agtacgagga ggattaccaa ttattgagga aacttccaga
     1261 accgatcgaa aaggtgatag actttagacc accgtacttg tttattccga agtcacttct
     1321 ttcgggtttc atctacaatc atttgagatt ttcttcaaaa ggtgtctgta tgagaatgga
     1381 tgatgtggaa aaaaccgatg atgttgtcac cgagatcatc aatatttcac caacagaatt
     1441 tcaagcgatt aaagcaaata ttaaatcaaa tatccaaggt aagtgtacta tcactccgtt
     1501 tttacatgtt tgttggtttg tatctcttca taaatggggt aaatttttca aaccattgaa
     1561 cttcgaatgg cttacggata tttttatccc cgcagattgc cgctcacaac taccagatga
     1621 tgatgaaatg agacagatgt acagatatgg cgctaacgtt ggatttattg acttcacccc
     1681 ctggataagc gaatctgaca tgaatgataa caaagaaaat ttttggccac ttattgagca
     1741 ctaccatgaa gtaatttcgg aagctttaag aaataaaaag catctccatg gcttagggtt
     1801 caatatacaa ggcttcgttc aaaaatatgt gaacattgac aaggtaatgt gcgatcgtgc
     1861 catcgggaaa agacgcggag gtacattgtt aagcaatgta ggtctgttta atcagttaga
     1921 ggagcccgat gccaaatatt ctatatgcga tttggcattt ggccaatttc aaggatcctg
     1981 gcaccaagca ttttccttgg gtgtttgttc gactaatgta aaggggatga atattgttgt
     2041 tgcttcaaca aagaatgttg ttggtagtca agaatctctc gaagagcttt gctccattta
     2101 caaagctctc cttttaggcc cttaataata ctagagccag gcatcaaata aaacgaaagg
     2161 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2221 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//