##gff-version 3
##sequence-region BBa_K1423006 1 2265
BBa_K1423006	.	engineered_region	527	2265	.	+	0	ID=BBa_J45199;Name=BBa_J45199
BBa_K1423006	.	ribosome_entry_site	527	541	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J45199
BBa_K1423006	.	engineered_region	527	541	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1423006	.	ribosome_entry_site	527	541	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1423006	.	ribosome_entry_site	534	538	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1423006	.	terminator	2225	2265	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45199
BBa_K1423006	.	engineered_region	2225	2265	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1423006	.	stop_codon	2258	2258	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1423006	.	polyA_site	2252	2265	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1423006	.	stem_loop	2232	2251	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1423006	.	terminator	2137	2216	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45199
BBa_K1423006	.	stem_loop	2148	2191	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1423006	.	engineered_region	2137	2216	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1423006	.	CDS	548	2128	.	+	0	ID=BBa_J45014;Name=BBa_J45014;Parent=BBa_J45199
BBa_K1423006	.	point_mutation	961	961	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_K1423006	.	stop_codon	2123	2128	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_K1423006	.	sequence_alteration	1695	1695	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_K1423006	.	CDS	548	2128	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_K1423006	.	engineered_region	1	518	.	+	0	ID=BBa_J33201;Name=BBa_J33201
BBa_K1423006	.	ribosome_entry_site	108	112	.	+	0	ID=annotation1902815;Name=rbs;Parent=BBa_J33201
BBa_K1423006	.	operator	36	54	.	+	0	ID=annotation1902812;Name=ArsR binding site;Parent=BBa_J33201
BBa_K1423006	.	promoter	57	62	.	+	0	ID=annotation1902813;Name=-35 sequence;Parent=BBa_J33201
BBa_K1423006	.	promoter	80	85	.	+	0	ID=annotation1902814;Name=-10 sequence;Parent=BBa_J33201
BBa_K1423006	.	CDS	119	472	.	+	0	ID=annotation1902816;Name=arsR;Parent=BBa_J33201
>BBa_K1423006
ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgct
tcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttg
ctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctct
cgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggt
aagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaac
aggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaa
atttagctaaacacatatgaattttcagatgtgttttatccgggtactagagattaaagaggagaaatactagatgaat
gaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgta
tgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgag
tgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgtt
ctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgatt
atatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagca
aatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattcct
tactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatct
ttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatat
taaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccg
atcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatt
tgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaa
tatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgttt
ttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggata
tttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgt
tggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgag
cactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcg
ttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaa
tgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaagga
tcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaaga
atgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata