BBa_K1423006
type: ComponentDefinition
persistent identity: BBa K1423006
display id: BBa_K1423006
version: 1
was generated by: iGEM to SBOL conversion
description: Arsenic Inducible Banana Odor Generator
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The arsenic promoter with the arsR repressor gene is naturally found in E. coli. The ribosome binding site, the ATF1 gene, and the double terminator combined sequence was obtained from the Biobrick BBa_J45199.
owned by: james
owned by: myers
mutable description: The aresnic inducible banana odor generator converts isoamyl alcohol to isoamyl acetate in the presence of arsenic.
discontinued: false
dominant: false
group u list: _1801_
m user id: 0
owner id: 22282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We digested the Biobricks with CIP to assure that the plasmids did not re-ligate to themselves.
star: false
creator: Kayla DeSanty
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J45199
component: BBa_J33201
sequence annotation: BBa_J33201
sequence annotation: BBa_J45199
sequence: https://synbiohub.org/public/igem/BBa_K1423006_sequence/1

BBa_J45199
type: Component
persistent identity: component2385476
display id: component2385476
version: 1
top level: BBa_K1423006
definition: ATF1 gener
access: public

BBa_J33201
type: Component
persistent identity: component2385461
display id: component2385461
version: 1
top level: BBa_K1423006
definition: BBa_J33201
access: public

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2385461
display id: annotation2385461
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
type: Range
persistent identity: range2385461
display id: range2385461
version: 1
top level: BBa_K1423006
direction: direction/reverse
start: 1
end: 518
orientation: inline

BBa_J45199
type: SequenceAnnotation
persistent identity: annotation2385476
display id: annotation2385476
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
component: BBa_J45199

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
type: Range
persistent identity: range2385476
display id: range2385476
version: 1
top level: BBa_K1423006
direction: direction/reverse
start: 527
end: 2265
orientation: inline

BBa_J33201
type: ComponentDefinition
persistent identity: BBa J33201
display id: BBa_J33201
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli chromosomal ars promoter with arsR repressor gene
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was derived by PCR using genomic DNA from E. coli JM109. Primers were designed based on the sequence reported by Diorio et al (1995), J. Bacteriol 177 (8), 2050-2056, Genbank accession X80057, GI:510824. The sequence given here is derived by sequencing the biobrick construct.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part consists of the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the arsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.
discontinued: false
dominant: true
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Note that this sequence includes the arsR gene encoding the ArsR repressor protein, which is thus negatively autoregulated. No additional parts are required for arsenate-induced expression from this part. In principle, this part should also function in hosts other than E. coli.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ArsR binding site
sequence annotation: -35 sequence
sequence annotation: -10 sequence
sequence annotation: rbs
sequence annotation: arsR
sequence: https://synbiohub.org/public/igem/BBa_J33201_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation1902812
display id: annotation1902812
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1
type: Range
persistent identity: range1902812
display id: range1902812
version: 1
top level: BBa_J33201
direction: direction/forward
start: 36
end: 54
orientation: inline

-35 sequence
type: SequenceAnnotation
persistent identity: annotation1902813
display id: annotation1902813
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1
type: Range
persistent identity: range1902813
display id: range1902813
version: 1
top level: BBa_J33201
direction: direction/forward
start: 57
end: 62
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1902814
display id: annotation1902814
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1
type: Range
persistent identity: range1902814
display id: range1902814
version: 1
top level: BBa_J33201
direction: direction/forward
start: 80
end: 85
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1902815
display id: annotation1902815
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1
type: Range
persistent identity: range1902815
display id: range1902815
version: 1
top level: BBa_J33201
direction: direction/forward
start: 108
end: 112
orientation: inline

arsR
type: SequenceAnnotation
persistent identity: annotation1902816
display id: annotation1902816
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1
type: Range
persistent identity: range1902816
display id: range1902816
version: 1
top level: BBa_J33201
direction: direction/forward
start: 119
end: 472
orientation: inline

ATF1 gener
type: ComponentDefinition
persistent identity: BBa J45199
display id: BBa_J45199
version: 1
was generated by: iGEM to SBOL conversion
description: Banana odor enzyme (ATF1) generator
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: coming
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is an intermediate to the protein generator BBa_45200.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: In stock
status: status/Available
bookmark: true
mutable notes: coming
star: true
creator: Andr?? Green II
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_B0010
component: BBa_J45014
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45199_sequence/1

BBa_B0030
type: Component
persistent identity: component1908009
display id: component1908009
version: 1
top level: ATF1 gener
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1908016
display id: component1908016
version: 1
top level: ATF1 gener
definition: BBa_B0010
access: public

BBa_J45014
type: Component
persistent identity: component1908015
display id: component1908015
version: 1
top level: ATF1 gener
definition: ATF1
access: public

BBa_B0012
type: Component
persistent identity: component1908018
display id: component1908018
version: 1
top level: ATF1 gener
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908009
display id: annotation1908009
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
type: Range
persistent identity: range1908009
display id: range1908009
version: 1
top level: ATF1 gener
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908015
display id: annotation1908015
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
type: Range
persistent identity: range1908015
display id: range1908015
version: 1
top level: ATF1 gener
direction: direction/reverse
start: 22
end: 1602
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908016
display id: annotation1908016
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
type: Range
persistent identity: range1908016
display id: range1908016
version: 1
top level: ATF1 gener
direction: direction/forward
start: 1611
end: 1690
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908018
display id: annotation1908018
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
type: Range
persistent identity: range1908018
display id: range1908018
version: 1
top level: ATF1 gener
direction: direction/forward
start: 1699
end: 1739
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: iGEM to SBOL conversion
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: In stock
status: status/Available
bookmark: true
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
star: true
creator: Andr?? Green II
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATF1
sequence annotation: a->g to eliminate EcoRI site
sequence annotation: t -> c
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
top level: ATF1
direction: direction/forward
start: 1
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
top level: ATF1
direction: direction/forward
start: 414
end: 414
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
top level: ATF1
direction: direction/forward
start: 1148
end: 1148
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
top level: ATF1
direction: direction/forward
start: 1576
end: 1581
orientation: inline

https://synbiohub.org/public/igem/BBa_K1423006_sequence/1
type: Sequence
persistent identity: BBa K1423006 sequence
display id: BBa_K1423006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccgggtactagagattaaagaggagaaatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33201_sequence/1
type: Sequence
persistent identity: BBa J33201 sequence
display id: BBa_J33201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45199_sequence/1
type: Sequence
persistent identity: BBa J45199 sequence
display id: BBa_J45199_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45014_sequence/1
type: Sequence
persistent identity: BBa J45014 sequence
display id: BBa_J45014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z