BBa_K1424000 1 BBa_K1424000 Prefix homologous recombination for Synechocystis ("Left flank") 2014-09-24T11:00:00Z 2015-05-08T01:10:23Z This part comes from genomic sequence of Synechocystis sp. PCC 6803 This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. Kunert, A., Hagemann, M. & Erdmann, N. (2000) Construction of promoter probe vectors of Synechocystis sp. PCC 6803 using the light-emitting reporter systems Gfp and LuxAB. J. Microbiol. Methods. 41, 185-194 Angermayr, S. A., Paszota, M. & Hellingwerf, K. J. (2012) Engineering a cyanobacterial cell factory for production of lactic acid. Appl. Environ. Microbiol. 78, 7098-7106 false false _1802_ 0 20667 9 In stock false n/a false Jacob Lamb annotation2400751 1 Synechocystis prefix homologous integration region range2400751 1 1 556 BBa_K1424001 1 BBa_K1424001 Suffix homologous recombination for Synechocystis ("Right flank") 2014-09-24T11:00:00Z 2015-05-08T01:10:23Z genomic This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. To be used with BBa_K1424000 false false _1802_ 0 20667 9 It's complicated false n/a false Jacob Lamb annotation2400764 1 Synechocystis suffix homologous integrating region range2400764 1 1 544 BBa_K1424003 1 BBa_K1424003 Kanamycin resistance for Synechocystis 2014-09-24T11:00:00Z 2015-05-08T01:10:23Z genomic DNA of kanamycin containing strain of Synechocystis sp. PCC 6803 The kanamycin resistance gene tn903 aph from e.coli for use with Synechocystis sp. PCC 6803. The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006). false false _1802_ 0 20667 9 In stock false N/A false Jacob Lamb annotation2400729 1 kanamucin resistance gene range2400729 1 120 920 annotation2400731 1 Synechocystis RBS + promotor region range2400731 1 1 110 BBa_K1424005 1 BBa_K1424005 Kan insert for Synechocystis 2014-09-25T11:00:00Z 2015-05-08T01:10:23Z composition of BBa_K1424000 + BBa_K1424002 + BBa_K1424001 Kanamycin resistance cassette for integration into the Synechocystis sp. PCC 6803 genome at the neutral site proposed by Kunert et al. (2000). false false _1802_ 0 20667 9 It's complicated false n/a false Jacob Lamb component2386911 1 BBa_K1424000 component2386913 1 BBa_K1424001 component2386912 1 BBa_K1424003 annotation2386912 1 BBa_K1424003 range2386912 1 565 1508 annotation2386911 1 BBa_K1424000 range2386911 1 1 556 annotation2386913 1 BBa_K1424001 range2386913 1 1517 2060 BBa_K1424000_sequence 1 cctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgacctgggggaaggggctcccctctggttagccaccaatcaaaataacagtggcggggatttatatgg BBa_K1424005_sequence 1 cctttgacaacaatgtggcctggaataacctgggggatttgtccaccaccacccaacgggcctacacttcggctattagcacagacacagtgcagagtgtttatggcgttaatctggaaaaaaacgataacattcccattgtttttgcgtggcccatttttcccaccacccttaatcccacagattttcaggtaatgcttaacacgggggaaattgtcaccccggtgatcgcctctttgattcccaacagtgaatacaacgaacggcaaacggtagtaattacgggcaattttggtaatcgtttaaccccaggcacggagggagcgatttatcccgtttccgtaggcacagtgttggacagtactcctttggaaatggtgggacccaacggcccggtcagtgcggtgggtattaccattgatagtctcaacccctacgtggccggcaatggtcccaaaattgtcgccgctaagttagaccgcttcagtgacctgggggaaggggctcccctctggttagccaccaatcaaaataacagtggcggggatttatatggtactagagcacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtatactagaggaccaagcccaatttcgtttgcgaatttacaccagcgccggtttttcccccgatggcattgccagtttactacccacagaatttgaacggtattttcaactccaagcggaagatattacgggacggacagttatcctaacccaaactggtgttgattatgaaattcccggctttggtctggtgcaggtgttggggctggcggatttggccggggttcaggacagctatgacctgacttacatcgaagatcatgacaactattacgacattatcctcaaaggggacgaagccgcagttcgccaaattaagagggttgctttgccctccgaaggggattattcggcggtttataatcccggtggccccggcaatgatccagagaatggtcccccagggccctttactgtgtccagtagtccccaggtaattaaggtaacggataccatcggccagcccaccaaagtctcctatgtggaagtggatggccccgtattgcgtaatcccttcagtggtactcccattgggcaagaggtgggtttagcgg BBa_K1424003_sequence 1 cacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgta BBa_K1424001_sequence 1 gaccaagcccaatttcgtttgcgaatttacaccagcgccggtttttcccccgatggcattgccagtttactacccacagaatttgaacggtattttcaactccaagcggaagatattacgggacggacagttatcctaacccaaactggtgttgattatgaaattcccggctttggtctggtgcaggtgttggggctggcggatttggccggggttcaggacagctatgacctgacttacatcgaagatcatgacaactattacgacattatcctcaaaggggacgaagccgcagttcgccaaattaagagggttgctttgccctccgaaggggattattcggcggtttataatcccggtggccccggcaatgatccagagaatggtcccccagggccctttactgtgtccagtagtccccaggtaattaaggtaacggataccatcggccagcccaccaaagtctcctatgtggaagtggatggccccgtattgcgtaatcccttcagtggtactcccattgggcaagaggtgggtttagcgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z